Patents by Inventor Jang B. Rampal
Jang B. Rampal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7271781Abstract: A method for species-specific identification of a Mycobacterium species is provided. Also disclosed is a method for identifying the species of Mycobacterium present in a sample. A device is disclosed for the detection of Mycobacterium. Mycobacterium-specific oligonucleotides are also disclosed, as are kits.Type: GrantFiled: March 1, 2001Date of Patent: September 18, 2007Assignees: The United States of America as Represented by the Secretary of the Department of Health and Human Services, Beckman Coulter, Inc.Inventors: Steven H. Fischer, Jang B. Rampal, Gary A. Fahle, Patricia S. Conville
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Patent number: 6861214Abstract: An assay article for detection biopolymers contained in a sample is described. The assay article includes a substrate and a biopolymer directly adsorbed on the surface of the substrate. A plurality of biopolymers may be adsorbed on the surface of the substrate to form an array. Also disclosed is a method of making the assay article. In the preferred method, an aminated polypropylene substrate is used. A biopolymer is contacted with the aminated substrate under a condition sufficient for direct adsorption of the biopolymer on the surface of the substrate. A method of detecting a target biopolymer contained in a sample is also disclosed. In this method, a substrate is contacted with either a probe or target biopolymer under a condition sufficient for a direct adsorption of either the probe or target biopolymer on the substrate to form a probe assay article or a target assay article.Type: GrantFiled: October 23, 2000Date of Patent: March 1, 2005Assignee: Beckman Coulter, Inc.Inventors: Jang B. Rampal, Robert S. Matson
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Publication number: 20040110129Abstract: A method for species-specific identification of a Mycobacterium species is provided. Also disclosed is a method for identifying the species of Mycobacterium present in a sample. A device is disclosed for the detection of Mycobacterium.Mycobacterium-specific oligonucleotides are also disclosed, as are kits.Type: ApplicationFiled: August 27, 2002Publication date: June 10, 2004Inventors: Steven H Fischer, Jang B Rampal, Gary A Fable, Patricia S Conville
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Publication number: 20040049351Abstract: A multiplexed immunosorbent assay can be performed in a microarray format on a plate. Capture molecules corresponding to the specific analytes are printed onto the bottom of the wells of chemically activated plates. The conditions are optimized for printing in terms of capture molecule spot density (mass and uniformity), coupling conditions, and blocking conditions. Samples containing analytes to be detected are delivered to the wells, allowed to incubate for a specific time after which unbound sample is removed by rinsing. Detection secondary capture molecules are pre-mixed and delivered to each well. Following incubation and rinse, signal generation reagents are added and the signals are detected.Type: ApplicationFiled: August 28, 2002Publication date: March 11, 2004Inventors: Robert S. Matson, Jang B. Rampal
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Publication number: 20040029156Abstract: An assay article for detection first biomolecules contained in a sample is described. The assay article includes a substrate having a modified surface and a first biomolecule directly adsorbed and immobilized on the modified surface of the substrate without linking moieties. A second biomolecule is bound to or adsorbed on the first biomolecule. Also disclosed is a method of making the assay article. A first biomolecule (other than an adhesive protein) is contacted with a modified surface of a substrate. The substrate is dried to directly adsorb the first biomolecule and immobilize it on the modified surface of the substrate without additional fixing steps to form an activated substrate. Then, a second biomolecule is contacted with the activated substrate under conditions sufficient for the first biomolecule to bind the second biomolecule.Type: ApplicationFiled: May 1, 2003Publication date: February 12, 2004Applicant: Beckman Coulter, Inc.Inventors: Robert S. Matson, Jang B. Rampal
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Patent number: 6013789Abstract: Disclosed herein is a method for attaching pre-synthesized oligonucleotides to a polypropylene support medium. Most preferably, a polypropylene film is aminated by a plasma discharge in the presence of ammonia gas. An oligonucleotide having a terminal phosphate is activated in the presence of an imidazole and a carbodiimide to form a phosphorimidazolide. The activated oligonucleotide becomes immobilized by forming a phosphoramidate bond with the aminated polypropylene. The invention can be used to construct oligonucleotide arrays for hybridization assays.Type: GrantFiled: February 20, 1998Date of Patent: January 11, 2000Assignee: Beckman Coulter, Inc.Inventor: Jang B. Rampal
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Patent number: 5985567Abstract: An improved method for detecting hybridized biomolecules is provided which comprises the steps of, providing a plurality of probe biomolecules on a solid support surface; pre-treating the plurality of probe biomolecules with an effective amount of a pre-treatment solution to produce a signal having an increased intensity of at least two (2) times, as compared to an identical detection method that does not include the pre-treating step; applying a buffer solution to the probe biomolecules; hybridizing the probe biomolecules with a plurality of target biomolecules to form a hybridized complex; and, developing and detecting the signal intensity. The developing step of the method may further include the steps of, treating the hybridized complex with a conjugating solution, and treating the conjugated, hybridized complex with a detection reagent.Type: GrantFiled: August 15, 1997Date of Patent: November 16, 1999Assignee: Beckman Coulter, Inc.Inventor: Jang B. Rampal
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Patent number: 5981185Abstract: A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization to a reverse dot blot array comprising strings of such repeats complementary to those found in particular nucleic acid targets (e.g., analyte PCR product). An addressable library (i.e., an indexed set) of complementary repeats is synthesized on a suitable support. Preferably, the support comprises a low fluorescent background support, thereby facilitating the use of non-radioisotopic modes of detection (such as fluorescence or chemiluminescence); particularly suitable in this regard is an aminated polypropylene support or similar material. Preferred arrays permit screening of DNA and RNA samples for complete sets of particular types of nucleotide repeat sequences (e.g., all nucleotide doublet or triplet repeats).Type: GrantFiled: May 28, 1997Date of Patent: November 9, 1999Assignee: Beckman Coulter, Inc.Inventors: Robert S. Matson, Peter J. Coassin, Jang B. Rampal, Charles Thomas Caskey
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Patent number: 5554501Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated biaxially oriented polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.Type: GrantFiled: October 29, 1993Date of Patent: September 10, 1996Assignee: Beckman Instruments, Inc.Inventors: Peter J. Coassin, Robert S. Matson, Jang B. Rampal
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Patent number: 5466351Abstract: A sample having both positively and negatively charged components may be separated during the same run by injecting a plug of sample at both ends of the capillary and applying an electric field in the capillary, where there is insufficient electroosmotic flow in the capillary to reverse sample migration direction, such as in the case of a gel-filled column.Type: GrantFiled: March 31, 1995Date of Patent: November 14, 1995Assignee: Beckman Instruments, Inc.Inventors: Sushma Rampal, Jang B. Rampal, James C. Osborne
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Patent number: 5462854Abstract: Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.Type: GrantFiled: April 19, 1993Date of Patent: October 31, 1995Assignee: Beckman Instruments, Inc.Inventors: Peter J. Coassin, Kenneth D. Konrad, Jang B. Rampal, Ronald M. Cook
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Patent number: 5437979Abstract: Procedures such as oligonucleotide, peptide or protein syntheses or sequencing, or immunological or other biospecific assays involving long sequential series of chemical reactions are performed on a solid phase support retained in a pipette tip. The manipulations involved in bringing the solid support into contact with the various reagents or reactants involved in the procedure are performed and controlled by automated instrumentation.Type: GrantFiled: August 24, 1992Date of Patent: August 1, 1995Assignee: Beckman Instruments, Inc.Inventors: Jang B. Rampal, Jon F. Harbaugh
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Patent number: 5429807Abstract: An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator in the form of a thick plate having in a surface at least one cavity in the form of an open chamber or channel is used for applying reagents to the surface of a solid phase support material. The applicator is positioned over the solid support material with the surface having the cavity sealed against the material surface. For each cavity, reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material, where each element of the array contains a population of biopolymers having identical sequence.Type: GrantFiled: October 28, 1993Date of Patent: July 4, 1995Assignee: Beckman Instruments, Inc.Inventors: Robert S. Matson, Peter J. Coassin, Jang B. Rampal, Edwin M. Southern
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Patent number: 5419874Abstract: The present invention is directed to a synthesis reaction column which can be conveniently implemented in post synthesis procedures. The reaction column is configured to allow easy connection to a syringe and post synthesis reagent container without requiring connection adapters.In one embodiment, the reaction column configured in the form of a cylindrical column containing the solid-phase support and having a narrow bore tube extending at one end. The tube functions as a piercing tip for accessing a septum sealed vial containing post synthesis reagent. In another embodiment, the reaction column is configured in the form of a cylindrical barrel enclosing the solid-phase support and having a threaded mount at one end. To complement the reaction column for post synthesis procedures, a vial having a complementary threaded mount at the opening is used for containing a reagent. This vial is conveniently threaded onto the end of the barrel without adapters.Type: GrantFiled: July 6, 1992Date of Patent: May 30, 1995Assignee: Beckman Instruments, Inc.Inventors: Peter J. Coassin, Edward G. Hanna, Carlton B. Mc Kinney, Jang B. Rampal, Glenn C. Sasaki, Stephen W. Wunderly
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Patent number: 4845101Abstract: Chemical compounds are provided including pyrazine diazohydroxides (I), as well as methods for their production, pharmaceutical compositions comprising the compounds, and methods of treatment using the compounds in dosage form. Compounds of the invention have pharmacological properties and are useful cytostatic agents and cell growth inhibiting agents.Type: GrantFiled: July 2, 1987Date of Patent: July 4, 1989Assignee: University of Alabama-Bd. of TrusteesInventors: David C. Baker, Elli S. Hand, Rudiger D. Haugwitz, Venkatachala L. Narayanan, Jang B. Rampal
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Patent number: 4709033Abstract: Chemical compounds are provided including pyrazine diazohydroxides (I), as well as methods for their production, pharmaceutical compositions comprising the compounds, and methods of treatment using the compounds in dosage form. Compounds of the invention have pharmacological properties and are useful cytostatic agents and cell growth inhibiting agents.Type: GrantFiled: November 13, 1984Date of Patent: November 24, 1987Assignee: Board of Trustees of the University of AlabamaInventors: David C. Baker, Elli S. Hand, Rudiger D. Haugwitz, Venkatachala L. Narayanan, Jang B. Rampal