Abstract: Provided herein are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas/guide RNA (gRNA) systems, for the transcriptional repression of Hepatitis B viral (HBV) genes to promote a cellular phenotype that leads to the reduction of HBV infection. In some embodiments, the epigenetic-modifying DNA-targeting systems bind to or target a target site of at least one gene or regulatory element thereof in a Hepatitis B viral DNA sequence in cell. In some aspects, the provided systems relate to the transcriptional repression of one or more Hepatitis B viral gene and/or regulatory element thereof. In some aspects, also provided herein are methods and uses related to the provided compositions, for example in repressing Hepatitis B viral replication and expression in connection with Hepatitis B infections.

Abstract: Provided herein are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas/guide RNA (gRNA) systems, for the transcriptional repression of Hepatitis B viral (HBV) genes to promote a cellular phenotype that leads to the reduction of HBV infection. In some embodiments, the epigenetic-modifying DNA-targeting systems bind to or target a target site of at least one gene or regulatory element thereof in a Hepatitis B viral DNA sequence in cell. In some aspects, the provided systems relate to the transcriptional repression of one or more Hepatitis B viral gene and/or regulatory element thereof. In some aspects, also provided herein are methods and uses related to the provided compositions, for example in repressing Hepatitis B viral replication and expression in connection with Hepatitis B infections.

Abstract: A system for dynamically mitigating resonance in a transport refrigeration unit (TRU) during a mission, having: a TRU controller configured for operating a TRU engine during the mission according to an operational baseline, and while operating the TRU engine, contemporaneously performing steps including: obtaining a first set of data that comprises real time measurements from one or more accelerometers installed in the TRU; converting the real measurements to a second set of data that comprises real time shock and vibration data; processing the second set of data in a control loop to determine an updated operational baseline that avoids resonance detected in the first set of data; and operating the TRU engine according to the updated operational baseline.

Abstract: A transport refrigeration unit system (26) for cooling a trailer compartment (24) is provided. The transport refrigeration unit system (26) includes an engine for controlling a cooling rate capacity, the engine operable at a nominal high speed and a nominal low speed. Also included is a controller (50) in operative communication with the engine to control an engine speed of the engine. Further included is a user interface (52) in operative communication with the controller (50), the user interface (52) providing a high capacity cooling mode to a user, wherein initiation of the high capacity cooling mode includes the engine operating at a speed greater than the nominal high speed to result in a high capacity cooling rate.

Abstract: Disclosed is a system for dynamically mitigating resonance in a transport refrigeration unit (TRU) during a mission, having: a TRU controller configured for operating a TRU engine during the mission according to an operational baseline, and while operating the TRU engine, and contemporaneously performing steps including: obtaining a first set of data that comprises real time measurements from one or more accelerometers installed in the TRU; converting the real measurements to a second set of data that comprises real time shock and vibration data; processing the second set of data in a control loop to determine an updated operational baseline that avoids resonance detected in the first set of data; and operating the TRU engine according to the updated operational baseline.

Abstract: An illustrative example refrigeration system includes a cooling apparatus, a generator that provides electrical power to the cooling apparatus, and an engine that provides power to the generator. A processor is configured to determine an ambient temperature, determine a load of the generator, determine a temperature of the generator, and determine whether the temperature of the generator exceeds a threshold, which is dependent on the ambient temperature and the load of the generator.

Abstract: A transport refrigeration unit system (26) for cooling a trailer compartment (24) is provided. The transport refrigeration unit system (26) includes an engine for controlling a cooling rate capacity, the engine operable at a nominal high speed and a nominal low speed. Also included is a controller (50) in operative communication with the engine to control an engine speed of the engine. Further included is a user interface (52) in operative communication with the controller (50), the user interface (52) providing a high capacity cooling mode to a user, wherein initiation of the high capacity cooling mode includes the engine operating at a speed greater than the nominal high speed to result in a high capacity cooling rate.

Abstract: A system for testing a particulate filter system includes a filter controller connected to the particulate filter system and a vehicular computer configured to determine at least one engine condition. The filter controller is configured to control a condition of a component of the particulate filter system and the vehicular computer is operatively connected to the filter controller to communicate therewith. The vehicular computer compares the condition of the component of the particulate filter system with the engine condition to determine whether the component of the particulate filter system is functioning properly.

Abstract: An area isolation system (AIS) is provided. The AIS may include a barrier adjacent to a work area, a barrier control panel (BCP) operatively connected to and disposed proximate the barrier, the BCP including a BCP controller in communication with a BCP short-range transceiver, the BCP controller configured to control the barrier, and a machine configured to autonomously operate within the work area. The machine may include a remote shutdown module (RSM) having an RSM controller in communication with an RSM short-range transceiver, and an RSM unique identifier preprogrammed into a memory associated with the RSM controller. The RSM short-range transceiver may be configured to send signals indicative of the RSM unique identifier to the BCP short-range transceiver when a machine radio zone associated with the RSM short-range transceiver intercepts with a BCP radio zone associated with the BCP short-range transceiver.

Abstract: A system for testing a particulate filter system includes a filter controller connected to the particulate filter system and a vehicular computer configured to determine at least one engine condition. The filter controller is configured to control a condition of a component of the particulate filter system and the vehicular computer is operatively connected to the filter controller to communicate therewith. The vehicular computer compares the condition of the component of the particulate filter system with the engine condition to determine whether the component of the particulate filter system is functioning properly.

Abstract: A heat exchanger (28 includes a first manifold (34), a second manifold (36) and a plurality of parallel heat transfer tubes (38). The first manifold (34) includes one or more manifold sections (58, 60, 63). A first one of the manifold sections (58) is connected to an inlet (64). The heat transfer tubes (38) extend between and fluidly connect the first and the second manifolds (34, 36). At least some of the heat transfer tubes (38) are disposed with the first manifold section (58).

Abstract: A transport refrigeration system having a structural framework supporting a refrigeration unit including a condenser heat exchanger supported in an upper region of the framework and an engine and an electric generator powered by the engine supported by the framework in an engine compartment in a lower region of the framework, includes a pair of vertically disposed condenser fan/motor assemblies disposed aft of the condenser heat exchanger in side-by-side relationship and above the engine compartment; and a flow partition extending vertically between the pair of condenser fan/motor assemblies.

Abstract: An engine driven generator (17) is provided with first and second stage fans (41,42) at one end thereof. The two-stage fan system draws in ambient air and forces it through the generator windings (38) and rotor-stator air gap (37,39) for the purpose of improving generator cooling. Stationary guide vanes (49) are disposed between the two fans (41,42) for the purpose of de-swirling the flow therebetween. The fans are of the centrifugal type and are preferably backward curved.

Abstract: An engine driven generator (17) is provided with first and second stage fans (41,42) at one end thereof. The two-stage fan system draws in ambient air and forces it through the generator windings (38) and rotor-stator air gap (37,39) for the purpose of improving generator cooling. Stationary guide vanes (49) are disposed between the two fans (41,42) for the purpose of de-swirling the flow therebetween. The fans are of the centrifugal type and are preferably backward curved.

Abstract: A transport refrigeration system having a structural framework supporting a refrigeration unit including a condenser heat exchanger supported in an upper region of the framework and an engine and an electric generator powered by the engine supported by the framework in an engine compartment in a lower region of the framework, includes a pair of vertically disposed condenser fan/motor assemblies disposed aft of the condenser heat exchanger in side-by-side relationship and above the engine compartment; and a flow partition extending vertically between the pair of condenser fan/motor assemblies.

Abstract: A transport refrigeration system includes a single frame for containing both a refrigeration condenser coil and an engine coolant radiator arranged in serial airflow relationship within a framework. Either one or the other may be removed and replaced without replacing the other.