Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications, including cell lineage analysis. Cell lineage analysis may comprise the use of one or more lineage tracing nucleic acid molecules. The disclosed methods may comprise using a lineage tracing nucleic acid molecule to identify a biological particle with one or more progenitor cells.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including generation of labeled macromolecules, including major mistocompatability complex (MHC) molecules, dextramers, etc. Labeled macromolecules may be generated using an in vitro transcription reaction. Labeled macromolecules may be generated in one or more partitions.

Abstract: A filtration cassette including a multilaminate array of sheets including filter sheets alternating with permeate sheet members and retentate sheet members, and a cross-flow filter device comprising a multiplicity of stacked filtration cassettes of such type. The improvements associated with the filtration cassettes described herein include, but are not limited to, at least one of: reinforced inlet(s) providing for longevity and improved cleanability of the cassettes; spacer permeate screens to limit throughput restriction; stainless steel or otherwise stiffened permeate sheets to prevent movement and increase flux; and stainless steel permeate sheets that can be used with ultrasonic transmission to minimize fouling of the filter sheets and extend cleaning cycles. Advantageously, the filtration cassettes are more resistant to higher temperatures than the filtration cassettes of the prior art.

Abstract: A method for detecting an unbalanced payload condition in a machine is disclosed. The method includes detecting, by a first sensor, a pressure exerted on each of one or more struts in the machine by a payload. The method further includes detecting, by a second sensor, one or more operational parameters associated with machine. Furthermore, method includes determining, by a controller, a center of gravity of the payload based on detected pressure, the one or more operational parameters, and one or more dimensions of the machine. Additionally, the method includes determining, by the controller, a force being exerted, by the payload, on each traction member of the machine based on the center of gravity of the payload. The method further includes detecting, by the controller, the unbalanced payload condition when the force, being exerted on at least one traction member of the plurality of traction members, exceeds a threshold value.

Abstract: The present invention provides for a method for separating starch from processing solutions containing starch containing plants or roots such as potatoes, sweet potatoes, wheat, corn, tapioca, yams, cassaya, sago, rice, pea, broad bean, horse bean, sorghum, konjac, rye, buckwheat and barley to provide commercially acceptable starch while reducing disposal of solid or liquid waste matter into landfills or water treatment facilities.

Abstract: The present invention provides for a method for separating starch from processing solutions containing starch containing plants or roots such as potatoes, sweet potatoes, wheat, corn, tapioca, yams, cassaya, sago, rice, pea, broad bean, horse bean, sorghum, konjac, rye, buckwheat and barley to provide commercially acceptable starch while reducing disposal of solid or liquid waste matter into landfills or water treatment facilities.

Abstract: Disclosed is an apparatus for bypassing and testing a fuel pump relay for a fuel pump of a vehicle. In an aspect, the apparatus includes a first pair of terminal pins configured to be inserted into the run/start signal fuse slot in the fuse box of the vehicle, a second pair of terminal pins configured to be inserted into a fuel pump fuse slot in the fuse box of the vehicle, a power input device configured to be conductively coupled to a power source of the vehicle, and a circuit configured to conductively couple, based on reception of electrical current from a run/start signal out terminal pin of the first pair of terminal pins, the power input device to a fuel pump terminal pin of the second pair of terminal pins to provide electrical current from the power input device to the fuel pump of the vehicle.

Abstract: Disclosed is an apparatus for bypassing and testing a fuel pump relay for a fuel pump of a vehicle. In an aspect, the apparatus includes a first pair of terminal pins configured to be inserted into the run/start signal fuse slot in the fuse box of the vehicle, a second pair of terminal pins configured to be inserted into a fuel pump fuse slot in the fuse box of the vehicle, a power input device configured to be conductively coupled to a power source of the vehicle, and a circuit configured to conductively couple, based on reception of electrical current from a run/start signal out terminal pin of the first pair of terminal pins, the power input device to a fuel pump terminal pin of the second pair of terminal pins to provide electrical current from the power input device to the fuel pump of the vehicle.

Abstract: The present invention relates to separation methods and systems for converting high concentrations of animal wastes into useful products, wherein the separation of the desired useful products is conducted with a cross-flow filtration system having the ability to the separate desired useful energy and/or products from both viscous and non-viscous medium.

Abstract: The present invention relates to separation of desired target products from biological, plant, and waste-type material, wherein the desired target products include renewable fuels such as ethanol, biobutanol, and biodiesel, wherein the separation is conducted with a cross-flow filtration system having the ability to separate desired products from both non-viscous and viscous medium.

Abstract: Materials and methods for treating a patient to express a therapeutic agent comprising administering a Kupffer cell-suppressing substance in combination with a vehicle for introducing, into the patient, an exogenous nucleic acid comprising a sequence for expression of the agent.

Abstract: The present invention relates to separation methods and systems for converting high concentrations of animal wastes into useful products, wherein the separation of the desired useful products is conducted with a cross-flow filtration system having the ability to the separate desired useful energy and/or products from both viscous and non-viscous medium.

Abstract: A method for detecting an unbalanced payload condition in a machine is disclosed. The method includes detecting, by a first sensor, a pressure exerted on each of one or more struts in the machine by a payload. The method further includes detecting, by a second sensor, one or more operational parameters associated with machine. Furthermore, method includes determining, by a controller, a center of gravity of the payload based on detected pressure, the one or more operational parameters, and one or more dimensions of the machine. Additionally, the method includes determining, by the controller, a force being exerted, by the payload, on each traction member of the machine based on the center of gravity of the payload. The method further includes detecting, by the controller, the unbalanced payload condition when the force, being exerted on at least one traction member of the plurality of traction members, exceeds a threshold value.

Abstract: The present invention relates to separation of desired target products from biological, plant, and waste-type material, wherein the desired target products include renewable fuels such as ethanol, biobutanol, and biodiesel, wherein the separation is conducted with a cross-flow filtration system having the ability to separate desired products from both non-viscous and viscous medium.

Abstract: An apparatus and method of a static image RAM drive is provided. The system includes a field programmable gate array (FPGA), a volatile memory drive, a non-volatile memory drive, and a power source. The non-volatile memory drive has a secure memory space. The secure memory space is programmed with an encryption key and an encrypted disk image. In response to a power cycle, the FPGA reads the encrypted disk image from the non-volatile memory, authenticates the disk image using the encryption key, decrypts the encrypted disk image, and writes the decrypted disk image to the volatile memory. The decrypted disk image is used to boot a computer. The computer is booted to a known good state each time the power is cycled.

Abstract: The present invention provides for a method for separating starch from processing solutions containing starch containing plants or roots such as potatoes, sweet potatoes, wheat, corn, tapioca, yams, cassaya, sago, rice, pea, broad bean, horse bean, sorghum, konjac, rye, buckwheat and barley to provide commercially acceptable starch while reducing disposal of solid or liquid waste matter into landfills or water treatment facilities.