Abstract: A sterilization tray for supporting medical instrumentation during the sterilization process. The tray includes a base having a perforated bottom wall to allow the passage of sterilization steam into the tray. A removable floor is fitted into the base and supported spacedly above the base bottom wall. The floor also includes perforations to allow for the passage of sterilization steam into the tray and above the instrumentation therein. One or more instrument holders are supported by the floor, extending upwardly from the floor in a direction opposite the location of the base bottom wall. The instrument holders can be removed from the floor with the floor being removed from the base, reversed in orientation and fitted into the base in its spaced relationship from the base bottom wall. The instrument holders are then repositioned upon the floor, extending upwardly from the opposite side of the floor.