Patents by Inventor Jason West
Jason West has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170043340Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.Type: ApplicationFiled: August 19, 2016Publication date: February 16, 2017Inventors: JASON A. A. WEST, JESSE THOMPSON
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Publication number: 20170002755Abstract: A fuel control system for an engine includes a closing module and a purge control module. The closing module commands closing of a purge valve in response an engine speed transitioning from greater than a predetermined speed to less than the predetermined speed while the purge valve is in an open state. The predetermined speed is less than a predetermined target speed of the engine and is greater than zero. The purge control module transitions the purge valve from the open state to a closed state in response to the command.Type: ApplicationFiled: August 31, 2015Publication date: January 5, 2017Inventors: Steven Ward MAJORS, Scott JEFFREY, Jason WEST
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Publication number: 20160348149Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5? to the target sequence, and a second probe-binding sequence 3? to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.Type: ApplicationFiled: May 2, 2016Publication date: December 1, 2016Inventors: Kenneth J. Livak, Jason A. A. West, Robert C. Jones
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Patent number: 9506845Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: GrantFiled: February 28, 2013Date of Patent: November 29, 2016Assignee: Fluidigm CorporationInventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A. A. West
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Patent number: 9440231Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.Type: GrantFiled: August 8, 2008Date of Patent: September 13, 2016Assignee: FLUIDIGM CORPORATIONInventors: Jason A.A. West, Jesse Thompson
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Publication number: 20160251714Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.Type: ApplicationFiled: February 26, 2016Publication date: September 1, 2016Inventors: Carolyn G. Conant, Tze Howe Charn, Jason A. A. West, Xiaohui Wang
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Patent number: 9429500Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: GrantFiled: February 28, 2013Date of Patent: August 30, 2016Assignee: Fluidigm CorporationInventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A. A. West
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Patent number: 9353406Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5? to the target sequence, and a second probe-binding sequence 3? to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.Type: GrantFiled: October 24, 2011Date of Patent: May 31, 2016Assignee: Fluidigm CorporationInventors: Kenneth J. Livak, Jason A. A. West, Robert C. Jones
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Patent number: 9304065Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: GrantFiled: February 28, 2013Date of Patent: April 5, 2016Assignee: Fluidigm CorporationInventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A. A. West
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Publication number: 20160025761Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.Type: ApplicationFiled: March 14, 2014Publication date: January 28, 2016Inventors: Jason A. A. West, Brian Fowler
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Publication number: 20150337295Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.Type: ApplicationFiled: May 8, 2015Publication date: November 26, 2015Inventors: JASON A.A. WEST, BRIAN FOWLER, TZE-HOWE CHARN, CHRISTIAN F. JOHNSON, MARC A. UNGER
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Patent number: 9028777Abstract: Provided are cartridges and systems for effecting automated extraction, isolation, and purification of cellular components—such as nucleic acids—from a cellular sample in assay-ready form. Also provided are related methods of effecting such sample processing.Type: GrantFiled: March 23, 2011Date of Patent: May 12, 2015Assignee: Fluidigm CorporationInventors: Kyle W. Hukari, Jason A. A. West, Michael A. Shultz, Richard Milson, Nikolas M. Isely, Michael C. DeRenzi
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Publication number: 20130302807Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: ApplicationFiled: February 28, 2013Publication date: November 14, 2013Inventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A.A. West
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Publication number: 20130302884Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: ApplicationFiled: February 28, 2013Publication date: November 14, 2013Inventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A.A. West
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Publication number: 20130302883Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: ApplicationFiled: February 28, 2013Publication date: November 14, 2013Inventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A.A. West
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Publication number: 20130295602Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: ApplicationFiled: February 28, 2013Publication date: November 7, 2013Inventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A.A. West
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Publication number: 20130296196Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.Type: ApplicationFiled: February 28, 2013Publication date: November 7, 2013Inventors: Brian Fowler, Jake Kimball, Myo Thu Maung, Andrew May, Michael C. Norris, Dominique G. Toppani, Marc A. Unger, Jing Wang, Jason A.A. West
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Patent number: 8450095Abstract: The present invention relates generally to systems and methods for processing a biological sample that result in a physical change, such as reacting two molecules together to form a reaction product or for use in lysing viruses or biological cells for analysis using biological assay systems. As such, the present invention relates both to breaking apart biological species such as viruses and cells, as well as the formation of reactants from one or more reactive species. The sample has a volume in the range from about 1 microliter to 10 milliliters. The sample is processed by applying pressure, and either sonic energy or thermal energy to the sample, wherein the pressure achieved is usually at least 24 atmospheres, and the temperature of the sample is usually raised to at least 50° C.Type: GrantFiled: July 14, 2010Date of Patent: May 28, 2013Assignee: Fluidigm CorporationInventors: Kyle W. Hukari, Jason A. A. West
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Patent number: 8426135Abstract: Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.Type: GrantFiled: November 30, 2011Date of Patent: April 23, 2013Assignee: Sandia National LaboratoriesInventors: Jason A. A. West, Kyle W. Hukari, Kamlesh D. Patel, Kenneth A. Peterson, Ronald F. Renzi
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Publication number: 20120128548Abstract: Provided are microfluidic devices and methods for fabricating and bonding such devices. Also provided are kits for analyzing analyte-containing samples and for lysing cells.Type: ApplicationFiled: August 8, 2008Publication date: May 24, 2012Applicant: ARCXIS BiotechnologiesInventors: Jason A.A. West, Jesse Thompson