Abstract: The embodiments herein provide a dental floss holder for holding dental floss filament. The dental floss holder has a floss filament for removing the tiny food particles from a space between the teeth. A handle is provided for supporting two legs holding the floss filament in a spool container enclosed with a cap. Two levers are used to hold and release the floss filament from a dispenser. The grooves are provided at the junction of two legs for passing the floss filament. The consumed floss filament is cut with a metal blade. An elastic rubber is provided to prevent an entry of water from into the floss filament. The lever is rotated in anticlockwise direction to release the floss filament for replacing the consumed floss filament with new floss filament while the lever is rotated in clockwise direction to cut the consumed floss filament with the metal blade.