Abstract: It relates to compositions produced by microorganism cell cultures, including microorganism-free compositions as well as compositions comprising inactivated microorganisms. It also relates to methods for obtaining the compositions produced by microorganism cell cultures and to agricultural compositions comprising them. It also relates to the use of the compositions of the invention as plant growth promoting agents and to methods for promoting stimulatory activity on plants comprising administering to the plant with these compositions.

Abstract: The invention relates to a method for changing the development pattern, increasing the growth and starch accumulation, changing the structure of starch and increasing the resistance to water stress in plants. The method involves culturing plants in an atmosphere containing volatile elements emitted by a microorganism, without there being any physical contact between the microorganism and the plant. The method is based on the discovery that the volatile elements emitted by Gram-positive or Gram-negative bacteria, yeasts and microscopic fungi stimulate an increase in the growth of plants in general, with an increase in the height, the number of leaves and/or the number of branches of the plant, as well as an increase in the accumulated starch and structural change of this biopolymer, and modification of the development pattern, with an increase in floral buds. An increased resistance to water stress can also be observed, in addition to an increase in starch in leaves separated from whole plants.

Abstract: The invention relates to a method for changing the development pattern, increasing the growth and starch accumulation, changing the structure of starch and increasing the resistance to water stress in plants. The method involves culturing plants in an atmosphere containing volatile elements emitted by a microorganism, without there being any physical contact between the microorganism and the plant. The method is based on the discovery that the volatile elements emitted by Gram-positive or Gram-negative bacteria, yeasts and microscopic fungi stimulate an increase in the growth of plants in general, with an increase in the height, the number of leaves and/or the number of branches of the plant, as well as an increase in the accumulated starch and structural change of this biopolymer, and modification of the development pattern, with an increase in floral buds. An increased resistance to water stress can also be observed, in addition to an increase in starch in leaves separated from whole plants.

Abstract: A process for the production of transgenic plants that have a high starch content and yield and a high amylose/amylopectin ratio. The alpha-1,4-glucan phosphorylases (GPs) catalyze the reversible cutting of bonds ?-1,4 of the non-reducing ends of homopolysaccharides with at least 5 glucose molecules such as starch, maltodextrin and glycogen, leading to production of glucose-1-phosphate. The GPs in bacteria and animal cells are responsible for the breakdown of glycogen. Although the increase in GP activity leads to a reduction in intracellular levels of glycogen in bacteria and animal cells, this invention discloses the production of plants that have high starch levels and yields and high amylose/amylopectin ratio, as result of the expression of genes coding for GPs.

Abstract: A transgenic plant that overexpresses sucrose synthase. The transgenic plant has a genetic construct that encodes a sucrose synthase peptide.

Abstract: The invention relates to a method for changing the development pattern, increasing the growth and starch accumulation, changing the structure of starch and increasing the resistance to water stress in plants. The method involves culturing plants in an atmosphere containing volatile elements emitted by a microorganism, without there being any physical contact between the microorganism and the plant. The method is based on the discovery that the volatile elements emitted by Gram-positive or Gram-negative bacteria, yeasts and microscopic fungi stimulate an increase in the growth of plants in general, with an increase in the height, the number of leaves and/or the number of branches of the plant, as well as an increase in the accumulated starch and structural change of this biopolymer, and modification of the development pattern, with an increase in floral buds. An increased resistance to water stress can also be observed, in addition to an increase in starch in leaves separated from whole plants.

Abstract: A transgenic plant that overexpresses sucrose synthase. The transgenic plant has a genetic construct that encodes a sucrose synthase peptide.

Abstract: An isolated sucrose synthase peptide. Also, a method of preparing ADPglucose by incubating the isolated sucrose synthase peptide with ADP in suitable conditions and then isolating and purifying the ADPG produced. Also, an assay kit for the spectrophotometric, fluorimetric or amperometric determination of sucrose, which kit includes the isolated sucrose synthase peptide. Also, a method of producing a transgenic plant that overexpresses sucrose synthase by inserting a genetic construct containing a DNA fragment that encodes the sucrose synthase peptide into a vector and transferring to a plant genome, and a transgenic plant obtained thereby.

Abstract: Process for the production of transgenic plants that have high content and yield of starch and biomass. The starch synthases (SSs) in plants (including SSIV) and glycogen synthase (GlgA) in bacteria catalyse the transfer of the glucosidic part of the ADP-Glucose molecule (the activated donor of glucose) to a pre-existing ?(1, 4)-glucan. However, in contrast to the other SSs, SSIV is able to add glucose units to maltotriose. Also, in contrast to other soluble SSs, SSIV is bound to the starch granule. This invention describes for the first time how to obtain plants that have high levels and yields of starch and biomass as a consequence of the expression of genes coding for SSIV.

Abstract: A process for the production of transgenic plants that have a high starch content and yield and a high amylose/amylopectin ratio. The alpha-1,4-glucan phosphorylases (GPs) catalyze the reversible cutting of bonds ?-1,4 of the non-reducing ends of homopolysaccharides with at least 5 glucose molecules such as starch, maltodextrin and glycogen, leading to production of glucose-1-phosphate. The GPs in bacteria and animal cells are responsible for the breakdown of glycogen. Although the increase in GP activity leads to a reduction in intracellular levels of glycogen in bacteria and animal cells, this invention discloses the production of plants that have high starch levels and yields and high amylose/amylopectin ratio, as result of the expression of genes coding for GPs.

Abstract: Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of ADPglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of ADPglucose and starch A method is described for efficient production of large quantities of soluble recombinant SS in its active form, by expression of the gene that encodes SS in a strain of Escherichia coli. The expression vector used means that the recombinant SS produced possesses a histidine tail which facilitates its quick purification. In addition it describes sequences of mutated versions of the gene of SS that encode isoforms of SS suitable for the production of ADPG. Making use of the “wild-type” and “mutated” versions of recombinant SS, an efficient method is described for production of ADPG and UDPG. It also describes the use of SS for the production of assay kits for the determination of sucrose.

Abstract: What are disclosed are a purified novel enzyme protein, UGPPase, which naturally occurs in animal cells, its production by means of recombinant technology, an antibody to the enzyme protein, a method of carrying out ELISA for measurement of the amount of UGPPase in an analyte and a method for determination of UDPG in a sample. The one-way enzyme protein catalyses hydrolysis of UDP-glucose, the precursor molecule of glycogen, into glucose-1-phosphate (G1P) and uridine 5?-monophosphate (UMP).

Abstract: A purified novel enzyme protein, UGPPase, which naturally occurs in animal cells, its productions of recombinant technology, an antibody to the enzyme protein, a method of carrying out ELISA for measurement of the amount of UGPPase in an analyte and a method for determination of UDPG in a sample. The one-way enzyme protein catalyses hydrolysis of UDP-glucose, the precursor molecule of glycogen, into glucose-1-phosphate (G1P) and uridine 5?-monophosphate (UMP).

Abstract: A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucleotide in the plant whereby to form the transgenic plant. The transgenic plant has a reduced starch content and a higher resistance to salinity than the plant before the transforming step.

Abstract: What are disclosed are a purified novel enzyme protein, UGPPase, which naturally occurs in animal cells, its production by means of recombinant technology, an antibody to the enzyme protein, a method of carrying out ELISA for measurement of the amount of UGPPase in an analyte and a method for determination of UDPG in a sample. The one-way enzyme protein catalyses hydrolysis of UDP-glucose, the precursor molecule of glycogen, into glucose-1-phosphate (G1P) and uridine 5?-monophosphate (UMP).

Abstract: Bacterial ADPglucose pyrophosphatase, method of production, use in the manufacture of testing devices and in the production of transgenic plants and bacteria. AGPPase is an enzyme that catalyzes the hydrolysis of ADPG (adenosine diphosphate glucose). The enzyme obtained from microbial extracts is used in testing devices for determining levels of ADPG, based on the G1P (glucose-1-phosphate) released by the reaction catalyzed by AGPPase. Partial amino acid sequences of the enzyme, the sequence of the gene that codes for it and the derived protein are also described. Finally, the production of transgenic plants and bacteria that overexpress the gene of AGPPase is described. The bacteria do not accumulate glycogen, whereas the plants obtained possess a high content of soluble sugars, low starch content and high resistance to high concentrations of salts and to high temperatures.

Abstract: Plant ADPglusose pyrophosphatases, process for production thereof, use in the production of assay devices and in obtaining transgenic plants.

Abstract: An isolated sucrose synthase peptide. Also, a method of preparing ADPglucose by incubating the isolated sucrose synthase peptide with ADP in suitable conditions and then isolating and purifying the ADPG produced. Also, an assay kit for the spectrophotometric, fluorimetric or amperometric determination of sucrose, which kit includes the isolated sucrose synthase peptide. Also, a method of producing a transgenic plant that overexpresses sucrose synthase by inserting a genetic construct containing a DNA fragment that encodes the sucrose synthase peptide into a vector and transferring to a plant genome, and a transgenic plant obtained thereby.