Abstract: A chemiluminescent detection system, as well as kits and microfluidics devices containing same, are disclosed. Methods of using the system, kits, and devices are also disclosed.

Abstract: A liquid deposition system comprises a liquid delivery assembly including a dispensing probe having a sidewall including a first end having a liquid port and a second end having a tip, and a flow path opening at the tip and fluidly connected with the liquid port. The system includes a gas injection assembly with a manifold having a gas nozzle, a nozzle opening, and a gas port, the gas nozzle configured to eject a substantially laminar gas stream so that the gas stream travels through a travel path, the tip of the dispensing probe extending into the travel path. The liquid port is fluidly connectable with a liquid source and the gas port is fluidly connectable with a pressurized gas source, so that a liquid micro droplet is formed at the tip. The laminar gas stream separates the micro droplet from the tip and carries it through the travel path.

Abstract: A chemiluminescent detection system, as well as kits and microfluidics devices containing same, are disclosed. Methods of using the system, kits, and devices are also disclosed. The first, second, and third antibodies or binding fragments thereof may be provided in any form that allows these antibodies/binding fragments to function in accordance with the presently disclosed and claimed inventive concept(s). For example, each of the first, second, and third antibodies/binding fragments may be a polyclonal antibody/binding fragment or a monoclonal antibody/binding fragment.

Abstract: A liquid deposition system comprises a liquid delivery assembly including a dispensing probe having a sidewall including a first end having a liquid port and a second end having a tip, and a flow path opening at the tip and fluidly connected with the liquid port. The system includes a gas injection assembly with a manifold having a gas nozzle, a nozzle opening, and a gas port, the gas nozzle configured to eject a substantially laminar gas stream so that the gas stream travels through a travel path, the tip of the dispensing probe extending into the travel path. The liquid port is fluidly connectable with a liquid source and the gas port is fluidly connectable with a pressurized gas source, so that a liquid micro droplet is formed at the tip. The laminar gas stream separates the micro droplet from the tip and carries it through the travel path.

Abstract: Provided is a method of preparing dye-based reference materials useful for calibrating or qualifying instrument systems that are diagnostic spectroscopically for hemoglobin and CO-ox fractions. The dye-based reference materials are non-proteinaceous and may be interpreted by CO-oximeter instrument systems as providing a spectrum substantially equivalent to the spectrum of blood, particularly with regard to hemoglobin and hemoglobin CO-ox fractions. Dye-based reference materials may be formulated to provide CO-ox fractions approximating those seen in a clinical setting, including normal physiological CO-ox fractions. The dye-based reference materials may be combined with other ingredients thus making the materials useful as references for pH and blood gas instruments and/or electrolyte instruments in addition to CO-oximeter instrument systems.

Abstract: Provided is a method of preparing dye-based reference materials useful for calibrating or qualifying instrument systems that are diagnostic spectroscopically for hemoglobin and CO-ox fractions. The dye-based reference materials are non-proteinaceous and may be interpreted by CO-oximeter instrument systems as providing a spectrum substantially equivalent to the spectrum of blood, particularly with regard to hemoglobin and hemoglobin CO-ox fractions. Dye-based reference materials may be formulated to provide CO-ox fractions approximating those seen in a clinical setting, including normal physiological CO-ox fractions. The dye-based reference materials may be combined with other ingredients thus making the materials useful as references for pH and blood gas instruments and/or electrolyte instruments in addition to CO-oximeter instrument systems.