Abstract: An imaging apparatus is provided for imaging tissue samples substantially beneath the surface of the tissue sample. The apparatus includes an objective lens and a window defining a tissue contacting surface in pressure contacting relationship with the surface of the tissue sample when the tissue sample is imaged by the objective lens to view tissue structures for pathological applications. The objective lens focuses an illumination beam through the window to the tissue sample and receives returned reflected light of the beam representative of one or more sections of the tissue sample. The apparatus enables a method for in vivo observation of tissue for diagnosis of conditions substantially beneath the surface of the tissue sample. Both two and three-dimensional imaging may be provided for diagnosis and location of basal cell carcinomas and melanomas, and so as to enable visualization of tumor borders prior to excision.

Abstract: A system and method for enhancing images of ex-vivo or in-vivo tissue produced by confocal microscopy, optical coherence tomography, two-photon microscopy, or ultrasound, is provided by applying to the tissue a solution or gel having an effective concentration of citric or other alpha-hydroxy acid which enhances tissue structures, such as cellular nuclei, in such images. Such concentration may be 3–20% acid, and preferably 5% acid.

Abstract: A colorimeter capable of calibrating color monitors, whether having cathode ray tube or liquid crystal (LCD) displays, is provided by a photometric array of photodetector and optical filter pairs. The filters include long-pass, edge filters which cover overlapping regions at the upper end of the visible spectrum and a filter which covers the entire visible spectrum. The outputs of the photodetectors are digitally synthesized to provide a response which mimics the response established by the Commission Internationale de 1′Eclairage (CIE) xyz (bar) functions almost perfectly. The response which is mimicked may be represented by the CIE color matching functions. The pairs and the associated components are mounted on a printed circuit board captured in a clamshell housing and having an array of apertures which define angularly constrained fields of view of a surface from which the light, to be colormetrically analyzed, emanates.

Abstract: A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. With the tissue specimen in the enclosed cavity of the cassette, the edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen.

Abstract: A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. With the tissue specimen in the enclosed cavity of the cassette, the edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen.

Abstract: A handheld imaging microscope for imaging tissue samples substantially beneath the surface of the tissue sample. The microscope includes an objective lens and a window defining a tissue contacting surface in pressure contacting relationship with the surface of the tissue sample when the tissue sample is imaged by the objective lens to view tissue structures for pathological applications. The objective lens focuses an illumination beam through the window to the tissue sample and receives returned reflected light of the beam representative of one or more sections of the tissue sample.

Abstract: A colorimeter capable of calibrating color monitors, whether having cathode ray tube or liquid crystal (LCD) displays, is provided by a photometric array of photodetector and optical filter pairs. The filters include long-pass, edge filters which cover overlapping regions at the upper end of the visible spectrum and a filter which covers the entire visible spectrum. The outputs of the photodetectors are digitally synthesized to provide a response which mimics the response established by the Commission Internationale de l'Eclairage (CIE) xyz (bar) functions almost perfectly. The response which is mimicked may be represented by the CIE color matching functions. The pairs and the associated components are mounted on a printed circuit board captured in a clamshell housing and having an array of apertures which define angularly constrained fields of view of a surface from which the light, to be colormetrically analyzed, emanates.

Abstract: A robust spectrophotometer (also known as a color spectrometer or colorimeter) is self contained in a housing which is adapted to be held-held and has all of the electrical, optical and electro optic elements mounted on a board captured within the housing at one end of which light from a sample is restricted to an object area and projected after being dispersed spectrally, as with a reflection grating, to an image area at a photodetector via a lens which has an optical axis and converges the dispersed light at the image area. The dispersive element is mounted on an arm having a pivot laterally offset from the dispersive element's surface where a diverging beam of light from the object area is incident and is deflected to the image area. The geometry is such that the dispersive element may be rotated to a position where the beam is specularly deflected (zeroth order diffraction), and the spectrometer calibrated when the dispersive element is in the specular reflection/deflection position.

Abstract: A hand held microsurgical instrument for applying laser energy to selected locations (sites) in an area under the skin (or other exposed translucent tissue) to provide localized photothermolysis of underlying tissue at these sites, is described. The laser energy is focused into a spot within the tissue. This spot is of sufficiently small size so that the energy density is sufficient to provide surgical or treatment effects within the tissue without damaging the surface tissue. In dermatology, for example, the technique can be used to destroy endothelial cells in blood vessels which are desired to be removed, such spider veins (nevi) in the skin, hair follicles to prevent hair growth therefrom, or other microsurgical procedures. The area is visualized while the laser beam is steered, using a deflection system, in X and Y coordinates.

Abstract: A spectrophotometer which is highly manufacturable at minimum cost nevertheless provides precision of measurement of spectra components of light which is projected therein by maintaining precise optical alignment of optical and electrical components thereof. These components are mounted in a module which is contained in a housing having an entrance aperture which defines an object area for light the spectrum of which is measured by a photodetector in the module at an image area. The module has a base plate provided by a printed circuit board on which a closed wall encompasses an area (a corral) on one side of the circuit board. The wall is a one piece structure which extends to the vicinity of the edge of the board. It is assembled with the board as a unitary structure so that the assembly is made torsionally rigid and resists bending in the plane of the board. The module may be of sufficiently small size so as to be located in a housing which is hand held, thereby providing a hand-held spectrophotometer.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A handheld confocal imaging system for in vivo observation of dermal and subdermal tissue allows diagnosis of conditions substantially beneath the surface of the skin. A confocal head has optics which scan the tissue so as to provide images of vertical sections of the tissue. Both two and three dimensional imaging may be provided for diagnosis and location of basal cell carcinomas and melanomas, and so as to enable visualization of tumor borders prior to excision.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A miniature scan engine module for bar code reading and data collection systems utilizes a light source 44 and a light collector 42, which in one embodiment, are flexurally supported on a platform 62 which reciprocates 88 on pivots defined by flexures 46, 48 or 96. The optical collector faces the bar code and pivots with the scanning beam source (a laser diode). A diffraction grating or Fresnel lens on the surface of the optical collector which faces the code directs the incoming light so that it propagates internally in a substrate within the body of the collector, i.e. in the optic itself and without air paths which require additional volume in the module, to a photodetector. The scan engine can be configured so that it occupies a volume of less than 1 cubic inch. A data collection system, in the form of a portable terminal 10, has a housing which provides the handle 12 of a scanning accessory.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A spectrophotometer which is highly manufacturable at minimum cost nevertheless provides precision of measurement of spectra components of light which is projected therein by maintaining precise optical alignment of optical and electrical components thereof. These components are mounted in a module which is contained in a housing having an entrance aperture which defines an object area for light the spectrum of which is measured by a photodetector in the module at an image area. The module has a base plate provided by a printed circuit board on which a closed wall encompasses an area (a corral) on one side of the circuit board. The wall is a one piece structure which extends to the vicinity of the edge of the board. It is assembled with the board as a unitary structure so that the assembly is made torsionally rigid and resists bending in the plane of the board. The module may be of sufficiently small size so as to be located in a housing which is hand held, thereby providing a hand-held spectrophotometer.

Abstract: In order to control the width of the scanning beam in the scanning direction over a significant range in front of the scanner, an optical assembly including a phase mask is utilized through which the beam passes after being configured into curved wavefronts, as by a lens which provides a focus in the vicinity of the far end of the range. The F/ number of the optical assembly is high and the cone of the beam in the scanning direction is small, for example, less than 5.degree.. The mask is preferably transparent and has a region which may be rectangular, elliptical or circular forming a step through which the center of the beam passes which imposes a phase change with respect to the phase of the wavefronts which do not propagate via the region. The phase change may be uniform or in the form of a weak quadratic phase variation in this region of the mask which changes phase and thus slightly refocuses the beam.

Abstract: A miniature scan engine for scanning a beam of monochromatic (laser) light across a bar code and receiving return light at a detector that provides electrical signals representing the code, utilizes an integral assembly of a laser (laser diode), the detector and a light collector mounted on flexures and rotated so as to scan the beam and receive the return light from the code. The collector is a body of material transmissive at the laser wavelength. The body presents an orientable surface so that the return light is incident at or close to perpendicular to the surface. In the body, parallel to the surface, is a volume hologram grating having Bragg planes arranged in rings around a center region in alignment with the detector. The angles of the Bragg planes vary progressively and decreases in a direction toward the center region so that light incident on the collector at angles of incidence, which vary from perpendicular to the surface by about + or -5.degree.

Abstract: A robust spectrophotometer (also known as a color spectrometer or colorimeter) is self contained in a housing which is adapted to be held-held and has all of the electrical, optical and electro optic elements mounted on a board captured within the housing at one end of which light from a sample is restricted to an object area and projected after being dispersed spectrally, as with a reflection grating, to an image area at a photodetector via a lens which has an optical axis and converges the dispersed light at the image area. The dispersive element is mounted on an arm having a pivot laterally offset from the dispersive element's surface where a diverging beam of light from the object area is incident and is deflected to the image area. The geometry is such that the dispersive element may be rotated to a position where the beam is specularly deflected (zeroth order diffraction), and the spectrometer calibrated when the dispersive element is in the specular reflection/deflection position.

Abstract: A hand held microsurgical instrument for applying laser energy to selected locations (sites) in an area under the skin (or other exposed translucent tissue) to provide localized photothermolysis of underlying tissue at these sites, is described. The laser energy is focused into a spot within the tissue. This spot is of sufficiently small size so that the energy density is sufficient to provide surgical or treatment effects within the tissue without damaging the surface tissue. In dermatology, for example, the technique can be used to destroy endothelial cells in blood vessels which are desired to be removed, such spider veins (nevi) in the skin, hair follicles to prevent hair growth therefrom, or other microsurgical procedures. The area is visualized while the laser beam is steered, using a deflection system, in X and Y coordinates.