Abstract: The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof.

Abstract: The invention is related to an inducible promoter for improved and regulated gene expression, useful in synthetic biology and metabolic engineering. In particular, the present invention relates to a nucleotide sequence comprising the regulatory regions of an erythritol- and erythrulose-inducible promoter in yeast and uses thereof in an expression system thus allowing an improved and regulated gene expression and production of gene product.

Abstract: The present invention relates to a method for obtaining a mutant strain of oleaginous yeast which is useful as a template strain of yeast for obtaining other mutant strains of oleaginous yeast which are capable of producing an unusual fatty acid. The present invention also relates to the mutant strains of yeast obtained by said method.

Abstract: The invention relates to a method for enhancing the erythritol and/or erythrulose productivity and/or yield of an erythritol and/or erythrulose-producing yeast strain, such as Yarrowia lipolytica, comprising inhibiting in said yeast strain the expression or the activity of an endogenous L-erythrulose kinase and/or erythritol dehydrogenase. The invention also relates to a mutant yeast strain obtained by said method.

Abstract: The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof.

Abstract: This invention relates to the use of a yeast strain overexpressing at least the following genes: the ALK3 gene, at least one of genes ADH2 and ADH5 and at least one of genes FALDH3 and FALDH4, or gene FA01, for the fermentation-based production of carboxylic diacids.

Abstract: The invention relates to a method for obtaining a mutant Yarrowia yeast strain capable of growing on D-galactose as sole carbon source, comprising over expressing in said strain a galactokinase, a galactose-1-phosphate uridyl transferase, an UDP-glucose-4 epimerase and a galactose mutarotase. The invention also relates to a mutant Yarrowia strain obtained by said method.

Abstract: The invention relates to a method for obtaining a mutant oleaginous yeast strain capable of growing on cellobiose as carbon source, comprising overexpressing in said strain two ?-glucosidase enzymes further comprising a N-terminal signal peptide. The invention also relates to a mutant yeast strain obtained by said method.

Abstract: The present invention relates to oleaginous yeast strains overexpressing a hexokinase gene, wherein said strains are capable of accumulating lipids. Methods for obtaining said strains as well as methods for producing lipids are also disclosed.

Abstract: The present invention relates to a mutant yeast strain, in which at least the expression or the activity of the 2-methyl-citrate dehydratase is inhibited, and to the use of said strain for the production of lipids and of citric acid.

Abstract: The present invention relates to a method for obtaining a mutant strain of oleaginous yeast which is useful as a template strain of yeast for obtaining other mutant strains of oleaginous yeast which are capable of producing an unusual fatty acid. The present invention also relates to the mutant strains of yeast obtained by said method.

Abstract: This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a Yarrowia strain including the steps of: (a) cultivating a Yarrowia strain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming said Yarrowia strain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors.

Abstract: The subject of the present invention is a novel mutant yeast strain, particularly a Yarrowia lipolytica strain, capable of accumulate a large quantity of lipids. The said strain does not express the GUT2 gene. According to one variant, the invention relates to mutant yeast strains, particularly Yarrowia lipolytica strains, which do not express the GUT2 gene and which furthermore do not carry out the ?-oxidation of lipids. Preferably, the mutant strains which do not express the GUT2 gene and which furthermore do not carry out ?-oxidation of lipids do not express the POX 2 through POX 5 genes, most preferably the POX 1 through 6 genes. The subject of the invention is also a method for producing the strains according to the invention and the use of the said strains in the production of lipids.

Abstract: The present invention relates to a novel mutant strain of yeast, particularly a strain of Yarrowia lipolytica, which is capable of accumulating a large quantity of lipids. Said strain is deficient in the beta-oxidation of fatty acids and overexpresses the GPD1 gene. Preferably, the mutant strains, which do not express the GUT2 gene and which are deficient for the beta-oxidation of fatty acids, do not express the POX2 to POX5 genes, very preferably the POX2 to 6 genes. The invention also relates to a method for obtaining the strains of yeast according to the invention and to the use of said strains in the production of lipids.

Abstract: The present invention concerns a method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation, comprising culturing a recombinant strain of a Yarrowia sp. comprising a heterologous gene coding for a hydroxyacyl-CoA dehydratase, under control of regulatory elements allowing expression of the said heterologous gene in the said Yarrowia sp. The invention also concerns the recombinant Yarrowia sp.

Abstract: This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a Yarrowia strain including the steps of: (a) cultivating a Yarrowia strain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming said Yarrowia strain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors.

Abstract: This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a Yarrowia strain including the steps of: (a) cultivating a Yarrowia strain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming said Yarrowia strain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors.

Abstract: The invention concerns a method for producing dicarboxylic acids (DCA) with long hydrocarbon chains, also called diacids, which consists in culturing a mutant strain of Yarrowia lipolytica obtained by mutagenesis directed and more particularly disrupted at least for the POX2, POX3, POX4 and POX5 genes encoding acyl-CoA oxydase, in a medium consisting essentially of an energetic substrate including at least one carbon source and one nitrogen source and in subjecting said strain to a bioconversion substrate selected among n-alkanes of at least 10 carbon atoms, fatty acids of at least 10 carbon atoms, their alkyl esters and natural oils.

Abstract: The invention concerns the integration of a gene of interest into the genome of a Yarrowia strain devoid of zeta sequences, by transforming said strain using a vector bearing zeta sequences.

Abstract: A process for the production of a polypeptide in which host cells transformed with DNA coding for a fusion protein comprising the polypeptide and a further peptide comprising a C-terminal secretion sequence are cultured to express and secrete the fusion protein. The C-terminal secretion sequence is, for example, the haemolysin C-terminal secretion sequence.