Abstract: The present invention concerns a cosmetic care method for the skin comprising the steps of applying, to the skin, a cosmetic composition comprising photoactive extracts of at least one microalgae, and exposing the area to which the composition has been applied to light. The invention also comprises a device for cosmetic care according to the method, and a kit comprising a cosmetic composition comprising photoactive extracts of at least one microalgae and a lighting device according to the invention. The invention finally comprises the use of the device according to the invention for an anti-aging and/or anti-wrinkle treatment and/or a treatment promoting the healing of the skin area.

Abstract: Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an ?-Mannosidase II and wherein at least one ?-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.

Abstract: A novel microalgal strain whose lipid production has been enhanced compared to the wild type. The lipid content produced by the microalgae is particularly rich in neutral lipids. The use of the microalgal strain and the lipids are also described.

Abstract: Disclosed is a transformed microalga including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an amino acid sequence including (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, the transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and the microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content.

Abstract: A novel microalgal strain whose lipid production has been enhanced compared to the wild type. The lipid content produced by the microalgae is particularly rich in neutral lipids. The use of the microalgal strain and the lipids are also described.

Abstract: Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an ?-Mannosidase II and wherein at least one ?-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.

Abstract: Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.

Abstract: The present invention concerns a transformed microalga producing a protein harboring a “high mannose” pattern of glycosylation in the plastid of the transformed microalga, wherein 1) the transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) the microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, the nucleic acid sequence encoding an amino acid sequence including (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of the protein, 3) the xylosyltransferases and fucosyltransferases of the microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of the microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of the microalga have not been inactivated.

Abstract: A transformed diatom includes a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an amino acid sequence including (i) an heterologous signal peptide and (ii) a polypeptide, the heterologous signal peptide leading to the secretion of the polypeptide in the extracellular medium of the transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, the method including the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of the culture and (iii) purifying the secreted polypeptide in the extracellular medium; and use of the transformed diatom for the secretion of a polypeptide in the extracellular medium.

Abstract: The invention relates to a CO2 fixation and organic waste processing method, wherein microorganisms (105) from a phytoplanktonic culture and organic waste (104) are processed inside a hydrolysis reactor (101); at least part of a liquid effluent (109) exiting the hydrolysis reactor is processed inside a methanation reactor (102); a liquid phase (127) and biogas to be purified (110) exiting Step (a?) are processed inside a phytoplanktonic microorganism culture unit (103); a CO2-containing gaseous effluent (113) is injected into the phytoplanktonic microorganism culture unit; an NH3 concentration under 0.5 g/L is maintained inside the methanation reactor; and a methane-enriched biogas is recovered upon exiting the phytoplanktonic microorganism culture unit. The invention also relates to a system for implementing this method.

Abstract: Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.