Abstract: Systems and methods of imaging are described. An imaging system comprises a light source configured to projecting a beam of light; a first diffraction grating configured to separating wavelengths of the projected beam of light; a first lens configured to focusing the wavelengths of the projected beam of light projecting from separated by the first diffraction grating; a reticle configured to altering each wavelength of light focused by the first lens; a second lens configured to collimating the wavelengths of light projecting from altered by the reticle; a second diffraction grating configured to multiplexing the collimated light projecting from collimated by the lens; and a third lens configured to projecting the multiplexed light onto an object plane, wherein the multiplexed light is used to generate an image of an object in the object plane.

Abstract: A multipass chirped pulse amplification system outfitted with a single-grating, simultaneous spatial and temporal focusing (SSTF) compressor platform is provided. Such a system provides the ability to vary the beam aspect ratio of an SSTF beam, and thus the degree of pulse-front tilt at a focus, while maintaining a net zero-dispersion system. The optical system may include a first optical element or set of optical elements that receives and then varies a pulse-front tilt of a light beam, and a second optical element or set of optical elements that focuses the light beam having the varied pulse-front tilt that is output by the first optical element or set of optical elements. The first optical element or set of optical elements includes an optical grating, two dihedrals, and a retroflector roof mirror.

Abstract: The direct integration of light and optical control into microfluidic systems presents a significant hurdle to the development of portable optical trapping-based devices. A simple, inexpensive fiber-based approach is provided that allows for easy implementation of diode-bars for optical particle separations within flowing microfluidic systems. Models have also been developed that demonstrate the advantages of manipulating particles within flow using linear geometries as opposed to individually focused point traps as traditionally employed in optical-trapping micromanipulation.

Abstract: A multipass chirped pulse amplification system outfitted with a single-grating, simultaneous spatial and temporal focusing (SSTF) compressor platform is provided. Such an system provides the ability to vary the beam aspect ratio of an SSTF beam, and thus the degree of pulse-front tilt at a focus, while maintaining a net zero-dispersion system. The optical system may include a first optical element or set of optical elements that receives and then varies a pulse-front tilt of a light beam, and a second optical element or set of optical elements that focuses the light beam having the varied pulse-front tilt that is output by the first optical element or set of optical elements. The first optical element or set of optical elements includes an optical grating, two dihedrals, and a retroflector roof mirror.

Abstract: A system, method, and device for re-orienting and/or deforming cells and other objects is provided. The system, method, and device may include a high-throughput setup that facilitates the ability to orient, deform, analyze, measure, and/or tag objects at a substantially higher rate than was previously possible. A relatively large number of cells and other objects can be deformed, by optical forces for example, as the cells and other objects a flowed through the system.

Abstract: The direct integration of light and optical control into microfluidic systems presents a significant hurdle to the development of portable optical trapping-based devices. A simple, inexpensive fiber-based approach is provided that allows for easy implementation of diode-bars for optical particle separations within flowing microfluidic systems. Models have also been developed that demonstrate the advantages of manipulating particles within flow using linear geometries as opposed to individually focused point traps as traditionally employed in optical-trapping micromanipulation.

Abstract: A system, method, and device for re-orienting and/or deforming cells and other objects is provided. The system, method, and device may include a high-throughput setup that facilitates the ability to orient, deform, analyze, measure, and/or tag objects at a substantially higher rate than was previously possible. A relatively large number of cells and other objects can be deformed, by optical forces for example, as the cells and other objects a flowed through the system.

Abstract: The invention provides microfluidic systems incorporating optical waveguides integrated, which can be used to optically interrogate particulate such as cells flowing through the system. The waveguides within these systems may be arranged to form optical traps, which may be used to power pumps and valves in the microfluidic systems, to trap and interrogate particles within these systems, and to sort trapped particles into different channels of microfluidic flow.

Abstract: An optical parametric amplifier pumped by ultrashort optical pulses provides time-gated image amplification or time-gated image frequency conversion, resulting in optical sectioning of an object under test and/or background rejection of improperly timed light. An ultrashort laser pulse of one frequency is used to illuminate an object under test. An ultrashort pulse light beam at a signal frequency transmitted through or scattered from the object is optically mixed with an ultrashort laser pulse at a pump frequency in a nonlinear optical medium. This mixing produces an amplified image of a particular optical section of the object at the signal frequency in addition to producing a frequency converted image of the same optical section at an idler frequency. This time-gated amplification can be used in conjunction with a confocal imaging system, or a conventional imaging system.

Abstract: An optical parametric amplifier pumped by ultrashort optical pulses provides time-gated image amplification or time-gated image frequency conversion, resulting in optical sectioning of an object under test and/or background rejection of improperly timed light. An ultrashort laser pulse of one frequency is used to illuminate an object under test. An ultrashort pulse light beam at a signal frequency transmitted through or scattered from the object is optically mixed with an ultrashort laser pulse at a pump frequency in a nonlinear optical medium. This mixing produces an amplified image of a particular optical section of the object at the signal frequency in addition to producing a frequency converted image of the same optical section at an idler frequency. This time-gated amplification can be used in conjunction with a confocal imaging system, or a conventional imaging system.

Abstract: A two-photon fluorescence microscope employs two laser beams having pulses of respective wavelengths .lambda..sub.2 and .lambda..sub.3, which cause two-photon emission of a fluorophore when the pulses are spatially and temporally overlapping. The pulses of the two beams of wavelengths .lambda..sub.2 and .lambda..sub.3 are combined at some crossing angle .theta. within the specimen, causing two-photon absorption within a line-shaped region during each instant of overlap. As the pulses pass through each other, the overlapping line-shaped region moves such that a slice of the fluorophore-containing specimen is excited by two-photon absorption during the overlap period. Lateral scanning is effected without moving parts by adjusting the relative delay of the pulses in the two beams. When the crossing angle .theta. is set to 0, i.e.