Abstract: The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Abstract: A degradation pick of the type used in such fields as road milling, mining, and trenching to engage and degrade tough materials such as asphalt, concrete, and rock may comprise a body attached at one end to a substantially cylindrical shaft. A hardened tip may also be attached to the body opposite the shaft. The hardened tip may comprise an axis offset from a central axis of the shaft. Such a degradation pick may be secured to an exterior of a rotatable drum or continuous chain so as to be repeatedly brought into contact with a surface of a material to be degraded. The body may comprise a protruding spine adjacent the hardened tip and opposite a direction of travel of the hardened tip when transported by a rotating drum or continuous chain.