Abstract: The present application discloses a method for treating a protein deficiency in the central nervous system of a subject in need thereof, comprising systemically administering to the subject a therapeutically effective dose of a fusion polypeptide comprising the first protein, wherein the fusion polypeptide comprises: (a) the first protein; (b) a second protein that provides extended circulation-lifetime in vivo and (c) blood brain barrier crossing facilitating peptide; wherein the fusion polypeptide crosses the blood brain barrier (BBB).

Abstract: Continuously Controlled Hollow fiber Bioreactor (CCHB) for the production of consistent quality cells or cell-derived products is provided. General components of the CCHB including a hollow fiber cell culture module, a new-medium chamber and a used-medium chamber, disposably attachable or detachable to the base devices such as rocking platform and circulation pumps. Quality of parameters including nutrient, oxygen, pH and temperature in the medium is optimally maintained during the production process. This ensures the controlled quality of the cell or cell-derived product throughout the process.