Abstract: The Apa I restriction fragment of the human erythropoitin gene, for producing high titers of biologically active hormone from stably transfected cell lines.

Abstract: A precast, prestressed concrete secondary containment vault comprised of a sloped, watertight bottom unit capable of holding a primary tank's entire contents, a collar unit sealed to the bottom unit by post-tensioned strands, top panels capable of supporting vehicles, a manhole providing access to permit direct inspection of the primary tank, and means for monitoring, ventilating and pumping the vault. Double-wall pipes for connecting the primary tank to external units and for directing any pipe leakage to the bottom unit are disclosed. The disclosed vault permits the use of less expensive, single-wall tanks which may be repaired in the vault or replaced by removing the top panels.