Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.

Abstract: Microalgae are potential energy resources for production of biofuels, such as biodiesel, ethanol, and butanol. A method for enhancing cell growth of microalgae enhances transgenic expression of a bicarbonate transporter (HCO3? transporter) in microalgae and thereby obtains a genetically modified microalgae capable of enhanced inorganic carbon fixation, efficient photosynthesis, and expeditious cell growth. The genetically modified microalgae are fit for use in biofuel production.

Abstract: A method for preparing a xylose-utilizing strain of Saccharomyces cerevisiae and the Saccharomyces cerevisiae are introduced. The preferred recombinant strain contains multiple copies of integrated xylose metabolic genes, and can rapidly ferment xylose to produce ethanol from synthetic medium and lignocellulosic raw materials. The xylose-utilizing strain is applicable for the cellulosic ethanol production industry and brewing industry.

Abstract: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

Abstract: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.

Abstract: A method for preparing a xylose-utilizing strain of Saccharomyces cerevisiae and the Saccharomyces cerevisiae are introduced. The preferred recombinant strain contains multiple copies of integrated xylose metabolic genes, and can rapidly ferment xylose to produce ethanol from synthetic medium and lignocellulosic raw materials. The xylose-utilizing strain is applicable for the cellulosic ethanol production industry and brewing industry.

Abstract: Disclosed is a method for increasing the concentration of xylose in lignocellulosic hydrolysate. The method includes the step of extruding lignocellulosic material and mixing the lignocellulosic material with diluted acid and the step of conducting hot-water extraction on the mixture. Hence, the concentration of the xylose in the lignocellulosic hydrolysate can achieve a high concentration of 60 g/L, and the yield of the xylose can reach to 90%.

Abstract: A hydrolysate-adapted yeast, Pichia stipitis INER 1128, is cultivated according to the present invention. The adapted yeast can effectively convert xylose into ethanol in lignocellulosic hydrolysate, which is not even detoxified. Well ethanol yield is obtained while xylose is not wasted and thus cost is reduced.

Abstract: Disclosed is a method for increasing the concentration of xylose in lignocellulosic hydrolysate. The method includes the step of extruding lignocellulosic material and mixing the lignocellulosic material with diluted acid and the step of conducting hot-water extraction on the mixture. Hence, the concentration of the xylose in the lignocellulosic hydrolysate can achieve a high concentration of 60 g/L, and the yield of the xylose can reach to 90%.

Abstract: The present disclosure is related to a method for increasing the ethanol concentration from the conversion of lignocellulose. The pretreated solid residues are mixed with ethanol-containing broth from the fermentation of xylose hydrolysate by Pichia stipitis and then are performed under the process of simultaneous saccharification and fermentation (SSF) with Sacharomyces cerevisiae and cellulase for converting cellulose to ethanol. The final ethanol concentration in broth as well as the ethanol productivity is increased at least 1.8 times in comparison of conventional process for lignocellulosic ethanol production.

Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.