Abstract: The present invention is a method of shoot apical meristem transformation for dicot plant via sufficient and micro wounding (SMW). The technical process includes: germinate the seeds in Petri dish or in the nutrient matrix which can be transplanted with the seedling; expose the shoot apical meristem by removing one cotyledon away; make sufficient and micro wounding transformation treatment in vivo to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 ?m in diameter for each one and 0.

Abstract: The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 ?m in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant.