Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.

Abstract: A ribbon carrier assembly, a welding device, and a welding method for preprocessing between a battery sheet and a ribbon are disclosed. The carrier assembly includes a carrier seat; a carrier body, rotatably connected to the carrier seat; and a driving assembly, connected to the carrier body and configured to control the rotation of the carrier body. At least one operation surface is arranged on the carrier body and is arranged as a curved surface. Adsorption holes are arranged on the operation surface. The battery sheet is adsorbed and fixed to the operation surface by applying a negative pressure to the adsorption holes. Through tins, dry joints between the battery sheet and the ribbon can be reduced during welding of the ribbon and the battery sheet. The battery sheet is bent on the curved surface to avoid warping or cracking of the battery sheet caused by excessive heating.

Abstract: The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.

Abstract: A dumbbell may include a handle, a first weighted portion and a second weighted portion, wherein the first weighted portion has a first cap and a first receiving space to receive one or more weighted units therein, and the second weighted portion has a second cap and a second receiving space to receive one or more weighted units therein. In one embodiment, the handle has a first threaded portion and a second threaded portion located at two opposite ends of the handle, and the first threaded portion is configured to threadedly engage with a first cap to secure the weighted units in the first receiving space, while the second threaded portion is configured is configured to threadedly engage with the second cap to secure the weighted units in the second receiving space.

Abstract: The present disclosure relates to a method for constructing a whole genome high-throughput sequencing library comprising the following steps: (1) extracting a sample gDNA; (2) fragmenting said sample gDNA by enzyme cleavage, filling ends of the gDNA and adding A base to the gDNA fragments to obtain an A-added gDNA; (3) connecting the A-added gDNA with a linker combination to obtain a connected produce, said linker combination comprises two parts: a Y-shaped reverse linker and a high GC clamp linker; (4) purifying said connected product to obtain a purified product; and (5) screening the fragment of said purified product to obtain a sequencing library. The present disclosure also relates to a kit for constructing a whole genome high-throughput sequencing library.

Abstract: The present invention relates to a method for a non-specific amplification of a natural short-fragment nucleic acid, comprising the following steps: (1) performing end repair on the natural short-fragment nucleic acid to obtain an end-repaired nucleic acid; (2) connecting the end-repaired nucleic acid to a double-stranded linker to obtain a ligation product, in which each strand of the double-stranded linker contains only three bases; (3) performing PCR amplification on the ligation product using a PCR primer labeled with deoxyuridine to obtain a PCR product, in which the PCR primer is completely or partially complementary to a strand of the double-stranded linker and contains only three bases; and (4) digesting the PCR product by using an enzyme having a deoxyuridine cleavage function, followed by digesting the PCR product by using an enzyme with both 5??3? polymerase activity and 3??5? exonuclease activity in the presence of a deoxynucleotide solution to obtain a non-specific amplification product of the na

Abstract: Provided in the present invention is a method for constructing a PacBio sequencing library, comprising the following steps: (1) obtaining a target double-stranded DNA; (2) adding a thermostable RNA ligase to respectively connect two ends of the double-stranded DNA to form a closed loop to obtain a dumbbell-shaped DNA library; (3) purifying the dumbbell-shaped DNA library; and (4) binding with sequencing primers and adding a DNA polymerase to obtain a PacBio sequencing library.

Abstract: The present invention provides a method for constructing a capture library, comprising the steps of: (1) obtaining fragmented DNAs; (2) connecting the fragmented DNAs with a Y-shaped linker to obtain a pre-library; (3) hybridizing the pre-library and a hybridization probe in the absence of a blocking sequence to obtain hybridization products; and (4) performing a PCR amplification on the hybridization products to obtain the capture library. The present invention also provides to a kit for carrying out the method.

Abstract: A method for quickly extracting long-fragment genomic DNAs by a single reaction tube, comprising the following steps: a) adding a lysis solution and protease K to a sample, to lyse cells and release the genomic DNAs; b) adding a precipitant to the reaction system of step a) to obtain a precipitate of the genomic DNAs; c) washing the obtained precipitate of the genomic DNAs using a washing solution; and d) dissolving the genomic DNAs using a dissolution solution. Also provided is a kit suitable for the method above.

Abstract: A physical exercise equipment set may include an elongated connecting bar having two ends and each end having a threaded engaging hole; a plurality of weighted portions; and two or more dumbbells. Each dumbbell has a handle having an elongated threaded portion at each of two opposite ends, a pair of weighted units located at two opposite ends of the handle, and a securing unit to secure each weighted unit in a predetermined position on the handle. For each of the two dumbbells, one of the weighted portions is taken out from one side of the handle, and the threaded portion is inserted into and threadedly engaged with each of the two threaded engaging holes of the connecting bar to form a barbell.

Abstract: A dumbbell may include a handle, a first weighted portion and a second weighted portion, wherein the first weighted portion and the second weighted portion are located at two opposite ends of the handle. In one embodiment, the first weighted portion has a cover and a receiving space, and the receiving space has a center receiving hole configured to axially receive one or more weighted units. Likewise, the second weighted portion has a cover and a receiving space having a center receiving hole that can be used to receive one or more weighted units. In one embodiment, the cover can be threadedly engaged with the outer peripheries of the first and second weighted portions to prevent the weighted units from moving or falling out while the dumbbell is being used.

Abstract: A dumbbell may include a handle, a first weighted portion and a second weighted portion, wherein the first weighted portion and the second weighted portion are located at two opposite ends of the handle. In one embodiment, the first weighted portion has a cover and a receiving space, and the receiving space has a center receiving hole and a plurality of surrounding receiving holes, each of which is configured to receive an elongated center weighted unit and one or more surrounding weighted units. Likewise, the second weighted portion has a cover and a receiving space having a center receiving hole and a plurality of surrounding receiving holes that can be used to receive each of the center and surrounding elongated weighted units. In one embodiment, the cover can be threadedly engaged with the outer peripheries of the first and second weighted portions.

Abstract: The present invention relates to a mutant of Argonaute protein lacking a DNA cleavage activity but having a DNA binding activity, wherein the mutation of the mutant is located in a PIWI domain. The present invention also relates to a use based on the protein mutant, especially in enrichment of a target DNA and construction of sequence libraries. Therefore, the present invention also relates to a method for enrichment of a target DNA, comprising the following steps: (a) designing a guide sequence for a specific sequence in the target DNA; (b) binding the mutant according to the present invention, the guide sequence and the target DNA to obtain a mutant-guide sequence-target DNA ternary complex; (c) capturing the mutant-guide sequence-target DNA ternary complex through a capture medium; and (d) separating the target DNA from the captured mutant-guide sequence-target DNA ternary complex to obtain an enriched target DNA.

Abstract: The present invention relates to a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also relates to a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.

Abstract: The present invention discloses a method for non-invasively detecting EGFR gene mutations in subjects, comprising the following steps: designing primers according to EGFR gene exons; extracting plasma DNAs in subjects; connecting the extracted plasma DNAs with tagging linkers; PCR pre-amplifying the tagging linkers connected plasma DNAs; cyclising the pre-amplified DNAs to obtain cyclised DNAs; PCR amplifying the cyclised DNAs using the designed primers; and high throughput sequencing the PCR amplified product and analyzing the EGFR gene mutations. The present invention also discloses a corresponding kit.

Abstract: Provided herein is a method of constructing a high throughput sequencing library for use in detecting chromosome copy number variation comprising mainly the following steps: (1) subjecting DNAs to be tested to random fragmentation by a double-strand DNA fragmentation enzyme; (2) end-filling and adding poly-adenine at the 3?end of the fragmented DNAs; (3) connecting the end-filled DNAs having a 3?end poly-adenine with sequencing linkers to obtain connected products; (4) purifying the connected products to obtain the sequencing library; wherein steps (1)-(3) are performed in a single reaction tube. Also provided is a kit for constructing a sequencing library for use in detecting chromosome copy number variation.

Abstract: The present invention relates to the field of noninvasive prenatal gene testing by high-through sequencing technologies. Particularly, the present application relates to a method for determining the content of cell-free fetal DNA in maternal peripheral blood.