Abstract: A method and apparatus for hydrocracking mineralized refuse pyrolysis oil. The method may use the following steps: (a) crushing and pyrolyzing mineralized refuse to obtain arene and alkane precursor biomass oil; (b) hydrogenating the arene and alkane precursor biomass oil obtained in step (a), and separating the obtained hydrocrackate to obtain arene and alkane; and (c) purifying, recovering and optimizing the arene and alkane obtained in step (b), and performing deep processing to produce naphtha, jet fuel, light diesel oil, and heavy diesel oil.

Abstract: The present disclosure provides devices and in vitro methods of developing three-dimensional neural tube-like tissues. In some aspects, the disclosure provides devices and methods of developing three-dimensional neural tube-like tissues comprising forebrain-like, midbrain-like, hindbrain-like, and spinal cord-like tissues. In particular, provided herein microfluidic devices and methods of using the same for generating neural tube-like tissues, such as neural-tube like tissues comprising forebrain-like, midbrain-like, hind-brain-like, and spinal cord-like tissues. In some embodiments, uses of such neural tube-like tissues for research, compound screening and analysis, disease modeling, and therapeutics are provided.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived embryo-like structures. In some embodiments, methods of generating embryo-like tissues from stem cells and the resulting tissues are provided. In some embodiments, uses of such tissues for research, compound screening and analysis, and therapeutics are provided. Accordingly, in some embodiments, provided herein is a method for preparing embryo-like tissue, comprising: a) introducing stem cells into a microfluidic device comprising a culture channel and a plurality of fluidic channels, wherein the stem cells are introduced to the culture channel of the microfluidic device; b) contacting the stem cells with basal medium via the plurality of fluidic channels for at least 18 hours (e.g., 36 hours) to generate the embryo-like tissue.

Abstract: A blood clot retraction assay device and methods of measuring whole-blood clot retractive forces during coagulation are described. When placed in proximity to a micro-beam, a blood droplet attaches thereto, applying a force to the micro-beam during clot formation. Using Piezoresistive micro-beams or other Piezoresistive components disposed upon such micro-beams, the device measures retractive force of a blood droplet based upon changes in resistance. The sensitivity of such measurements allows use of small quantities of blood for measurement, between 15 microliters and 300 microliters. The device further permits continuous measurement of coagulation, allowing measurement of distinct reaction, contraction development, and fibrinolysis phases for each blood sample.

Abstract: A preparation method and uses of a high-performance water-soluble acrylic resin with high solid content and low viscosity. The method polymerizes the free radical solution in a mixed solvent by a continuous method to produce an acrylic resin and the resin is rendered water-soluble through salification. Modification with versatate introduces a large branched structure. Silicone functional monomer is used to modify the acrylic resin. Amino resin is used as a curing agent to directly prepare a waterborne amino-acrylic coating with a simple process, and the coating has good hardness, fullness, water and alcohol resistance and salt spray resistance.

Abstract: Described herein are various embodiments directed to microfluidic cell culture devices, systems, and methods. Embodiments of devices and systems disclosed herein may be used to grow and characterize one or more phenotypes of a cell sample. An apparatus may include an apparatus including a substrate defining a cavity, and further include a scaffold disposed within the cavity. The substrate and the scaffold may collectively define a set of channels including a first channel and a second channel. The first channel may be configured to receive and culture a cell sample during use. The second channel may be configured to receive a fluid during use. The scaffold may be configured to permit diffusion of the fluid through the scaffold and into the first channel.

Abstract: A preparation method and uses of a high-performance water-soluble acrylic resin with high solid content and low viscosity. The method polymerizes the free radical solution in a mixed solvent by a continuous method to produce an acrylic resin and the resin is rendered water-soluble through salification. Modification with versatate introduces a large branched structure. Silicone functional monomer is used to modify the acrylic resin. Amino resin is used as a curing agent to directly prepare a waterborne amino-acrylic coating with a simple process, and the coating has good hardness, fullness, water and alcohol resistance and salt spray resistance.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Abstract: The present disclosure provides compositions and methods employing stem cell-derived cardiomyocytes. In some embodiments, compositions for assessing cardiotoxicity using 3-dimensional cultures of stem-cell cardiomyocytes are provided.

Abstract: A blood clot retraction assay device and methods of measuring whole-blood clot retractive forces during coagulation are described. When placed in proximity to a micro-beam, a blood droplet attaches thereto, applying a force to the micro-beam during clot formation. Using Piezoresistive micro-beams or other Piezoresistive components disposed upon such micro-beams, the device measures retractive force of a blood droplet based upon changes in resistance. The sensitivity of such measurements allows use of small quantities of blood for measurement, between 15 microliters and 300 microliters. The device further permits continuous measurement of coagulation, allowing measurement of distinct reaction, contraction development, and fibrinolysis phases for each blood sample.

Abstract: This invention provides a method and an apparatus for quickly continuously fractionating biomolecules, such as DNAs, proteins and carbohydrates by taking advantage of differential bidirectional transport of biomolecules with varying physico-chemical characteristics, for example size, charge, hydrophobicity, or combinations thereof, through periodic arrays of microfabricated nanofilters. The passage of biomolecules through the nanofilter is a function of both steric and electrostatic interactions between charged macromolecules and charged nanofilter walls, Continuous-flow separation through the devices of this invention are applicable for molecules varying in terms of any molecular properties (e.g., size, charge density or hydrophobicity) that can lead to differential transport across the nanofilters.

Abstract: This invention provides a method and an apparatus for quickly continuously fractionating biomolecules, such as DNAs, proteins and carbohydrates by taking advantage of differential bidirectional transport of biomolecules with varying physico-chemical characteristics, for example size, charge, hydrophobicity, or combinations thereof, through periodic arrays of microfabricated nanofilters. The passage of biomolecules through the nanofilter is a function of both steric and electrostatic interactions between charged macromolecules and charged nanofilter walls, Continuous-flow separation through the devices of this invention are applicable for molecules varying in terms of any molecular properties (e.g., size, charge density or hydrophobicity) that can lead to differential transport across the nanofilters.

Abstract: This invention provides a method and an apparatus for quickly continuously fractionating biomolecules, such as DNAs, proteins and carbohydrates by taking advantage of differential bidirectional transport of biomolecules with varying physico-chemical characteristics, for example size, charge, hydrophobicity, or combinations thereof, through periodic arrays of microfabricated nanofilters. The passage of biomolecules through the nanofilter is a function of both steric and electrostatic interactions between charged macromolecules and charged nanofilter walls, Continuous-flow separation through the devices of this invention are applicable for molecules varying in terms of any molecular properties (e.g., size, charge density or hydrophobicity) that can lead to differential transport across the nanofilters.