Abstract: An RF connector (100) for receiving a mating connector along a mating direction, includes an insulative housing (1), an outer conductor (3) retained with the insulative housing, and a central conductor (2) retained with the insulative housing. The outer conductor includes a tubular section (31) defining an axial line along the mating direction and a number of leg sections (32, 33) extending outwardly from a bottom of the tubular section. The central conductor includes a contact section (22) positioned within the tubular section along the mating direction, a radial section (21) extending outwardly from a bottom of the contact section along a radial direction perpendicular to the mating direction, and an extension section (24) extending out of the insulative housing. The extension section is connected with the radial section via a declined connection portion (23). The insulative housing extends below the radial section.

Abstract: An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.

Abstract: An electrical connector (100) includes a dielectric housing (1) having a base portion (11) and a tongue portion (12) extending from the base portion; a number of terminals (2) received in the housing; and an upper shield (31) and a lower shield (32) enclosing the insulative housing. The upper shield includes a main cover (310) located above the insulative housing and a pair of wing portions (312) extending oppositely, laterally relative to the main cover. The lower shield includes a main plate (320) located below the insulative housing and a pair of wings (321) extending oppositely, laterally relative to the main plate. One of the wing portion and corresponding wing defines a closed slot (3122) and the other one of the wing portion and the corresponding wing integrally, interiorly fits in the closed slot.

Abstract: An RF connector (100) for receiving a mating connector along a mating direction, includes an insulative housing (1), an outer conductor (3) retained with the insulative housing, and a central conductor (2) retained with the insulative housing. The outer conductor includes a tubular section (31) defining an axial line along the mating direction and a number of leg sections (32, 33) extending outwardly from a bottom of the tubular section. The central conductor includes a contact section (22) positioned within the tubular section along the mating direction, a radial section (21) extending outwardly from a bottom of the contact section along a radial direction perpendicular to the mating direction, and an extension section (24) extending out of the insulative housing. The extension section is connected with the radial section via a declined connection portion (23). The insulative housing extends below the radial section.

Abstract: An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.

Abstract: An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.

Abstract: An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.

Abstract: An electrical connector assembly (100) comprises an insulative body (1) integrally including a forward part (12) and a backward part (11). A main shell (32) includes a front pocket (320) enclosing the forward part (12), and a back segment (322) covering an upper portion of the backward part (11) of the body (1). A sub-shell (31) is assembled with the main shell (32), and includes a lower piece (316) covering a lower portion of the backward part (11). Therefore, the main shell (32), the sub-shell (31) are assembled with each other to provide a good shielding enclosure for the insulative body (1), where the contact terminals (2) are held therein.

Abstract: An electrical connector (100) includes a dielectric housing (1) having a base portion (11) and a tongue portion (12) extending from the base portion; a number of terminals (2) received in the housing; and an upper shield (31) and a lower shield (32) enclosing the insulative housing. The upper shield includes a main cover (310) located above the insulative housing and a pair of wing portions (312) extending oppositely, laterally relative to the main cover. The lower shield includes a main plate (320) located below the insulative housing and a pair of wings (321) extending oppositely, laterally relative to the main plate. One of the wing portion and corresponding wing defines a closed slot (3122) and the other one of the wing portion and the corresponding wing integrally, interiorly fits in the closed slot.

Abstract: An electrical connector assembly (100) comprises an insulative body (1) integrally including a forward part (12) and a backward part (11). A main shell (32) includes a front pocket (320) enclosing the forward part (12), and a back segment (322) covering an upper portion of the backward part (11) of the body (1). A sub-shell (31) is assembled with the main shell (32), and includes a lower piece (316) covering a lower portion of the backward part (11). Therefore, the main shell (32), the sub-shell (31) are assembled with each other to provide a good shielding enclosure for the insulative body (1), where the contact terminals (2) are held therein.

Abstract: A receptacle connector (100) includes a metal shield (3) having the cover (31) and an opposite base (32). The cover (31) includes a pair of top lateral wings (312). The base includes a pair of bottom lateral wings (322) for mating with the respective top lateral wings (312). The top lateral wing (312) defines a first portion of a whole lateral wing board, while the bottom lateral wing (322) defines a second portion adapted to contribute towards completing the whole lateral wing board. Thus, the metal shield (3) is integrated by the cover (31) and the base (32), each of which is molded by a single sheet.

Abstract: A receptacle connector (100) includes a metal shield (3) having the cover (31) and an opposite base (32). The cover (31) includes a pair of top lateral wings (312). The base includes a pair of bottom lateral wings (322) for mating with the respective top lateral wings (312). The top lateral wing (312) defines a first portion of a whole lateral wing board, while the bottom lateral wing (322) defines a second portion adapted to contribute towards completing the whole lateral wing board. Thus, the metal shield (3) is integrated by the cover (31) and the base (32), each of which is molded by a single sheet.

Abstract: The present invention relates to recombinant DNA encoding the OkrAI restriction endonuclease as well as OkrAI methylase, expression of OkrAI restriction endonuclease in E. coli cells containing the recombinant DNA.

Abstract: The present invention relates to recombinant DNA which encodes the BpmI restriction endonuclease as well as BpmI methyltransferase, expression of BpmI restriction endonuclease from E. coli cells containing the recombinant DNA. BpmI endonuclease is a fusion of two distinct elements with a possible structural domains of restriction-methylation-specificity (R-M-S). This domain organization is analogous to the type I restriction-modification system with three distinct subunits, restriction, methylation, and specificity (R, M, and S). Because BpmI is quite distinct to other type IIs restriction enzymes, it is proposed that BpmI belongs to a subgroup of type II restriction enzymes called type IIf (f stands for fusion of restriction-modification-specificity domains). The Type IIf group of restriction enzyme includes Eco57I, BpmI, GsuI, BseRI and some other restriction enzymes that cut downstream sequences at long distance, 10-20 bp downstream of recognition sequence, such as MmeI (N20/N18)).

Abstract: The present invention relates to recombinant DNA which encodes the NheI restriction endonuclease as well as NheI methyltransferase, expression of NheI restriction endonuclease from E. coli cells containing the recombinant DNA. An internal NdeI site in the nheIR gene was eliminated by a silent mutation. A new NdeI site was engineered at the start codon of nheIR gene. An NdeI-BamHI fragment containing nheIR gene was cloned into a T7 expression vector pAII17 and expressed in a premodified host ER2566 [pACYC-NheIM, pAII17-NheIR2]. The recombinant clone produces approximately 10 million units of NheI per gram of wet cells.

Abstract: The present invention relates to recombinant DNA molecules encoding NspHI restriction endonuclease and methylase and to method to use premodified E. coli K strain RR1 (.lambda.DE3) for overexpression of NspHI restriction endonuclease.

Abstract: BsrFI restriction enzyme was purified from Bacillus stearothermophilus to near homogeneity. The protein was sequenced to obtain its N-terminus amino acid sequence. A set of denegerate primers were synthesized based on the aa sequence. The first 18 codons encoding BsrFI restriction endonuclease (bsrFIR) was amplified by PCR and its coding sequence was obtained. The methylase selection method was used to clone BsrFI methylase gene (bsrFIM). Two clones were found to be resistant to BsrFI digstion. The entire insert in one clone was sequenced and the insert encodes the BsrFI methylase (M. BsrFI). In addition, a small truncated open reading frame adjacent to the methylase gene has homology to Cfr10I restriciton endonuclease in a BlastX homology search in Genbank database. BsrFI and Cfr10I are isoschizomer that recognizes and cleaves 5'R CCGGY3'.

Abstract: The methylase selection method was used to clone the BslI methylase gene (bslIM) from Bacillus species. A partially active BslI methylase lacking the 17 amino acid residues at the N-terminus was cloned in E. coli using expression vector pRRS. Inverse PCR was used to clone the missing portion of the BslI methylase. After cloning the complete BslI methylase gene and its upstream DNA sequences, a RadC homolog was found upstream of the BslI methylase. Because methylase gene and restriction endonuclease gene are located in proximity to each other in a particular restriction-modification system, efforts were made to clone the downstream DNA by inverse PCR. After two round of inverse PCR reactions two open reading frames (ORF) were found downstream of the BslI methylase gene. Expression of the first ORF (ORF1) in a T7 expression vector did not yield any active BslI endonuclease. Expression of the second ORF (ORF2) in E.

Abstract: The present invention relates to cloning recombinant DNA molecules encoding a multi-specific methylase gene (bssHIIM1), BssHII restriction endonuclease gene (bssHIIR), and the cognate BssHII methylase gene (bssHIIM2) from Bacillus stearothermophilus H3 E. coli. The BssHII multi-specific methylase gene was first cloned in a Sau3AI library using a modified pLITMUS28 vector (New England Biolabs, Inc., Beverly, Mass.) with two BssHII sites. Expression of the multi-specific BssHII methylase renders the two BssHII sites resistant to BssHII digestion. Surprisingly, the cloned methylase also modifies some other sites in addition to BssHII site (5'GCGCGC3'). The methylase also modifies BsrFI site (5'RCCGGY3') and HaeII site (5'RGCGCY3'); and partially modifies EagI site (5'CGGCCG3') and MIul site (5'ACGCGT3'). The beginning of the bssHIIR gene was cloned by using two degenerate primers based on the N-terminal amino acid sequence in PCR. The rest of the bssHIIR gene was cloned by inverse PCR.

Abstract: The present invention relates to isolated DNA coding for the restriction endonuclease SCaI as well as to a method for cloning methylase genes from Streptomyces into E. coli by a modification of the methylase selection method. At first, the standard methylase gene selection method was tried to clone the SCaI methylase gene using a high-copy-number cloning vector pUC19 during library construction. The SCaI methylase gene was refractory to cloning by using pUC19, presumably due to the poor expression of the SCaI methylase gene in E. coli. If the SCaI methylase is not efficiently expressed in E. coli, the SCaI sites on the plasmid will not be sufficiently modified by the methylase. As a consequence, the plasmid will be cleaved and lost in the plasmid library after SCaI endonuclease challenge. Since the standard methylase selection did not work, the "endo-blue method" was tried to clone the SCaI endonuclease gene.