Abstract: The present disclosure provides compositions and methods for enhanced expression of exogenous genes in eukaryotic cells. The method involves introducing into a mammalian cell an exogenous nucleic acid. wherein the exogenous nucleic acid intearates into a locus of the genome that comprises an extended methylation-free CpG island. Also provided are chromosomal loci, sequences for enhanced and stable expression of exogenous genes.

Abstract: The present disclosure discloses a method for producing raw cotton of machine-harvested long-staple cotton with a modal length of greater than or equal to 35 mm, including the steps: planting sea-island long-staple cotton and machining seed cotton, wherein planting the sea-island long-staple cotton includes preparation before machine harvesting, defoliation and ripening, choosing a cotton variety, etc.; and machining the seed cotton includes preparation before seed cotton ginning, mechanical separation of cotton seeds and raw cotton, and finishing the separated raw cotton. Raw cotton fibers obtained by the method of the present disclosure have main quality indexes that: a fiber length is 36.5-38.4 mm, a specific strength is 42.3-46.1 cN/tex, a uniformity is 86.2-87.0%, a foreign-fiber content is 0.2-0.4 g/t, and an impurity content is 2.6-3.4%.

Abstract: An energy storage system includes a battery cluster, a direct current converter, a controller, and at least one power conversion system. The energy storage system includes at least one direct current branch. The direct current branch includes a battery cluster and a direct current converter that are connected to each other. The direct current converter performs direct current conversion on a direct current provided by the battery cluster. Each battery cluster includes at least two energy storage modules that are connected in series. The controller controls the first equalization circuit, to balance electricity quantities of battery modules in the battery cluster, and allocates operating power to the power conversion system and the direct current converter. In this solution, impact of bucket effect on the energy storage system is reduced, so that a battery capacity can be more fully utilized.

Abstract: Embodiments of the present disclosure provide a power conversion system and a control method. The power conversion system includes multiple power modules, and each power module includes a first port, a second port, and a third port, where the first port of each power module is connected to an external device, second ports of the power modules are independent of each other and used as independent ports to output power, third ports of the power modules are connected in parallel to form a power bus and power flow of the third port of each power module is bidirectional.

Abstract: A power module includes N inverter units outputting N AC voltages and being coupled to N high-frequency AC terminals, wherein the N high-frequency AC terminals are cascaded and connected to a post-stage rectifier circuit. A phase-shift control method for the power module includes: setting at least two phase-shift sequences, wherein phase sequence numbers of the N AC voltages of the N inverter units are different in the at least two phase-shift sequences; in one switching period, controlling the N AC voltages of the N inverter units to shift a first angle according to a first phase-shift sequence of the at least two phase-shift sequences; and in another switching period, controlling the N AC voltages of the N inverter units to shift the first angle according to a second phase-shift sequence of the at least two phase-shift sequences.

Abstract: Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.

Abstract: A short-circuit protection apparatus includes a first detection branch, a second detection branch, and a controller. The first detection branch includes a first sampling resistor and a first sampling capacitor that is connected in parallel to the first sampling resistor. A difference between an absolute value of a second sampling voltage and an absolute value of a first sampling voltage is a first difference. The controller obtains a comparison result between an absolute value of a first sampling voltage at two terminals of the first sampling resistor and an absolute value of a second sampling voltage at two terminals of the second sampling resistor, and if a difference between the absolute value of the second sampling voltage and the absolute value of the first sampling voltage is a second difference and the second difference is less than the first difference, controls the target circuit to stop working.

Abstract: Provided is a method of inserting a polynucleotide sequence into a genome of a cell. The method comprises: generating a double-strand break at a target site of the genome; and introducing into the cell a virus. The virus comprises a nucleic acid comprising the polynucleotide sequence to be inserted or the complementary sequence thereof. The nucleic acid does not comprise a homologous arm or comprises very short (5˜25 bp) homologous arms corresponding to the target site. Also provided herein is a composition for inserting a polynucleotide sequence into a genome of a cell. The composition comprises a site-specific nuclease capable of generating a DNA double-strand break at a target site of the genome and a virus comprising a nucleic acid comprising the polynucleotide sequence or the complementary sequence thereof.

Abstract: Provided herein are methods and compositions for treating genetic blood cell diseases, e.g. sickle cell disease and thalassemia, by correcting genetic mutation or inserting exogenous globin gene using CRISPR/Cas system.

Abstract: Provided is a method for knocking in a gene of interest to a cell. The genome of the cell contains a negative selectable marker, e.g., a thymidine kinase gene flanked by a pair of recombinase recognition sites (RRS), e.g., attP. The method involves introducing into the cell a targeting construct that contains a gene of interest flanked by a second pair of RRS, e.g., attB. The targeting construct also contains in the vector backbone a negative selectable marker, e.g., thymidine kinase gene. When a recombinase recognizing the RRS is expressed, the recombination events between the two pairs of RRS result in the site-specific integration of the gene of interest in the genome of the cell. Upon selection based on the negative selectable marker, the parental cells, cells with undesired integration, e.g., random integration, or the integration of the vector backbone are removed.

Abstract: A power module includes N inverter units outputting N AC voltages and being coupled to N high-frequency AC terminals, wherein the N high-frequency AC terminals are cascaded and connected to a post-stage rectifier circuit. A phase-shift control method for the power module includes: setting at least two phase-shift sequences, wherein phase sequence numbers of the N AC voltages of the N inverter units are different in the at least two phase-shift sequences; in one switching period, controlling the N AC voltages of the N inverter units to shift a first angle according to a first phase-shift sequence of the at least two phase-shift sequences; and in another switching period, controlling the N AC voltages of the N inverter units to shift the first angle according to a second phase-shift sequence of the at least two phase-shift sequences.

Abstract: Provided herein are methods for producing a cell having chromosome translocation, deletion or reversion. In one embodiment, the method for producing a cell having chromosome translocation comprises introducing site-specific nucleases to the cell to create double strand breaks in a first and a second chromosome; generating an intermediate fusion chromosome comprising a first segment of the first chromosome, a selection region and the second segment of a second chromosome; and creating double strand breaks at sites that flank the selection region, thereby generating a fusion chromosome comprising at least part of the first segment of the first chromosome linked to at least part of the second segment of the second chromosome.

Abstract: The present disclosure provides a composition comprising a site-specific nuclease domain capable of cleaving a target DNA sequence; and a sequence-specific DNA binding domain capable of specifically binding to a recognition DNA sequence, wherein the site-specific nuclease domain operably links to the sequence-specific DNA binding domain.

Abstract: Embodiments of the present disclosure provide a power conversion system and a control method. The power conversion system includes multiple power modules, and each power module includes a first port, a second port, and a third port, where the first port of each power module is connected to an external device, second ports of the power modules are independent of each other and used as independent ports to output power, third ports of the power modules are connected in parallel to form a power bus and power flow of the third port of each power module is bidirectional.

Abstract: Provided herein are compositions and methods for genome editing and modification. In one embodiment, the composition comprises a regulatory gene expression construct that comprises a nucleic acid encoding an RNA comprising a sequence encoding a genome editing enzyme and a regulatory cassette operably linked to the sequence. In one embodiment, the regulatory cassette comprises a conditional exon and an aptamer domain which is capable of binding to an effector molecule to trigger a structural change of the RNA, thereby regulating splicing of the conditional exon and expression of the genome editing enzyme.

Abstract: The present disclosure provides a coagulation factor VIII(FVII) mimetic protein. The FVIII mimetic protein comprises (1) a coagulating factor IX (FIX/FIXa) binding domain comprising a first heavy chain variable region (VHI) and a first light chain variable region (VL1), wherein the VH1 and the VL1 are derived from an antibody specifically binding to FIX/FIXa; (2) a coagulation factor X (FX) binding domain comprising a second heavy chain variable region (VH2) and a second light chain variable region (VL2), wherein the VH2 and the VL2 are derived from an antibody specifically binding to FX; and (3) a membrane binding domain.

Abstract: A pixel structure, a method for forming the pixel structure, and a display screen are provided. The method includes: providing a substrate for forming an OLED device, the substrate having a first pixel area, a second pixel area, and a third pixel area; and forming a compensation layer on the substrate, the compensation layer having different thicknesses in the first pixel area, the second pixel area, and the third pixel area. In the present disclosure, the compensation layer is formed on the substrate, and the compensation layer has different thicknesses respectively in the first pixel area, the second pixel area and the third pixel area, so that the cavity of the first pixel area, the cavity of the second pixel area, and the cavity of the third pixel area can be individually controlled.

Abstract: Provided is a method of inserting a polynucleotide sequence into a genome of a cell. The method comprises: generating a double-strand break at a target site of the genome; and introducing into the cell a virus. The virus comprises a nucleic acid comprising the polynucleotide sequence to be inserted or the complementary sequence thereof. The nucleic acid does not comprise a homologous arm or comprises very short (5˜25 bp) homologous arms corresponding to the target site. Also provided herein is a composition for inserting a polynucleotide sequence into a genome of a cell. The composition comprises a site-specific nuclease capable of generating a DNA double-strand break at a target site of the genome and a virus comprising a nucleic acid comprising the polynucleotide sequence or the complementary sequence thereof.

Abstract: A shadow mask used for OLED evaporation and a manufacturing method therefor, and an OLED panel manufacturing method. The shadow mask used for OLED evaporation includes: a semiconductor substrate including a front surface and a back surface opposite thereto, a recess penetrating the front surface and the back surface being provided in the semiconductor substrate; and a grid film layer provided on the front surface of the semiconductor substrate. A number of openings arranged in an array are provided in the grid film layer. Each of the openings has an upper portion and a lower portion. A width of the upper portion is greater than a width of the lower portion. The recess exposes the number of openings in the grid film layer and the grid film layer between adjacent openings.

Abstract: Provided herein are compositions and methods for enhancing expression of SMN protein in a cell. In one embodiment, the composition comprises a site-specific nuclease targeting the ISS-N1 region of human SMN2 gene. Also provided are compositions and methods for treating or ameliorating spinal muscular atrophy.