Abstract: Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.

Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.

Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for promoting modification of the DNA sequence at a target site in a fungal host cell genome.

Abstract: Disclosed herein, inter alia, are cleavage-resistant antibodies and antibody-producing host cells as well as methods for making, using, and improving secretion of the same.

Abstract: The present compositions and methods relate to a beta-glucosidase from Glomerella graminicola, polynucleotides encoding the beta-glucosidase, and methods of making and/or use thereof. Formulations containing the beta-glucosidase may be suitable for use in hydrolyzing lignocellulosic biomass substrates.

Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.

Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.

Abstract: The present compositions and methods relate to a beta-glucosidase from Glomerella graminicola, polynucleotides encoding the beta-glucosidase, and methods of making and/or use thereof. Formulations containing the beta-glucosidase may be suitable for use in hydrolyzing lignocellulosic biomass substrates.

Abstract: The present compositions and methods relate to a beta-glucosidase from Melanocarpus albomyces, polynucleotides encoding the beta-glucosidase, and methods of making and/or using the same. Formulations containing the beta-glucosidase are suitable for use in numerous applications, including hydrolyzing lignocellulosic biomass substrates.

Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.

Abstract: A steel member (10) having wear and corrosion resistance through a combination of coatings is disclosed, the steel member (10) being a hardened and tempered steel member having a high tensile strength, at least one selected portion (18) of the steel member (10) coated by thermal spraying with a tungsten carbide-cobalt composition (22) to provide wear and corrosion resistance, the tungsten carbide-cobalt composition (22) being approximately 78-90% tungsten carbide and approximately 10-19% cobalt, and a sacrificial ceramic-metallic coating (24) on the steel member (10) to provide corrosion resistance for the steel member (10). A method of coating a steel member (10) is also disclosed.

Abstract: An hot shoe attachment for fitting a flash gun or the like to the hot shoe of a camera has a flanged body for fitting to the hot shoe with a locking ring screw threaded thereto for clamping it in place. Electrical contact means on the body on the attachment are resiliently urged towards and against the hot shoe when the attachment is fitted. As the locking ring is unscrewed to release the attachment, the contact means are simultaneously withdrawn from the hot shoe to avoid damage by contact therewith as the flash gun or the like is removed. Only when the locking ring is again screwed to its clamping position do the contact means again project from the body. The contact means are normally mounted in a bushing which itself has flanges engaged by the locking ring at least as the locking ring is unscrewed. Resilient urging means may be applied to the contact means, the bushing or both.