Abstract: The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

Abstract: Disclosed in the present disclosure are a method and system for controlling an air conditioner, the air conditioner, and a household appliance. The method comprises: data collected by a control device during an operation of the air conditioner is received, wherein the data comprises question information, and at least one of indoor image data and an operation status diagram of the air conditioner located indoors; the collected data is input to a training model to obtain an answer that corresponds to the question information; and the answer is played.

Abstract: Disclosed in the present disclosure are a method and system for controlling an air conditioner, the air conditioner, and a household appliance. The method comprises: data collected by a control device during an operation of the air conditioner is received, wherein the data comprises question information, and at least one of indoor image data and an operation status diagram of the air conditioner located indoors; the collected data is input to a training model to obtain an answer that corresponds to the question information; and the answer is played.