Abstract: The present invention belongs to the medical field, and relates to novel ?-lactamase inhibitors, for the treatment of bacterial infections in combination with ?-lactam antibiotics, including infection caused by drug resistant organisms and especially multi-drug resistant organisms. The present invention includes compounds according to formula (I): or pharmaceutically acceptable salts thereof, wherein M and R are as defined herein.

Abstract: The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

Abstract: The present disclosure discloses a recombinant Escherichia coli for producing rosmarinic acid and application thereof, belonging to the technical fields of genetic engineering and bioengineering. In the present disclosure, FjTA derived from Flavobacterium johnsoniae, endogenous hpaBC derived from E. coli, CbRAS derived from Coleus blumei, HPPR derived from Coleus scutellarioides, and Pc4CL1 derived from Petroselinum crispum are heterologously expressed in E. coli, realizing synthesis of rosmarinic acid. TcTAL derived from Trichosporon cutaneum and tyrC for removing feedback inhibition are introduced, further increasing synthesis throughput of caffeic acid, and PmLAAD derived from Proteus myxofaciens is heterologously expressed, realizing redistribution of L-DOPA. An endogenous gene menl is knocked out, improving the content and stability of a rosmarinic acid precursor. The recombinant strain constructed in the present disclosure can produce rosmarinic acid by fermentation at a yield of up to 511.

Abstract: Provided are a video pushing method, an apparatus, a device, and a storage medium. In an embodiment of the present disclosure, the number of times a user page is displayed on a user terminal is obtained; if the number of times is less than a preset value, a target video to be pushed to the user terminal is determined according to a popularity of a video in a storage system corresponding to a server; if the number of times is greater than a preset value, a target video to be pushed to the user terminal is determined according to feature information of a user and feature information of a video in the storage system corresponding to the server. Accordingly, the server can continuously push videos that meet the user's needs to the user terminal while the user is using the user page, thereby improving the user experience.

Abstract: Embodiments of the present disclosure provides a method for pushing video information, an apparatus, a device and a storage medium. In the embodiments of the present disclosure, the user behavior information in the user page at the current moment is acquired, and the related information of the target video that is pushed to the user terminal at the next moment is determined according to the user behavior information, so that the user terminal refreshes the user page according to the related information of the target video, thus the target video that is pushed by the server to the user terminal is more in line with the user's preference, therefore the user quickly finds the video that he/she likes in the user interface, thereby improving the user experience.

Abstract: Embodiments of the present disclosure provides a method for pushing video information, an apparatus, a device and a storage medium. In the embodiments of the present disclosure, the user behavior information in the user page at the current moment is acquired, and the related information of the target video that is pushed to the user terminal at the next moment is determined according to the user behavior information, so that the user terminal refreshes the user page according to the related information of the target video, thus the target video that is pushed by the server to the user terminal is more in line with the user's preference, therefore the user quickly finds the video that he/she likes in the user interface, thereby improving the user experience.

Abstract: Provided are a video pushing method, an apparatus, a device, and a storage medium. In an embodiment of the present disclosure, the number of times a user page is displayed on a user terminal is obtained; if the number of times is less than a preset value, a target video to be pushed to the user terminal is determined according to a popularity of a video in a storage system corresponding to a server; if the number of times is greater than a preset value, a target video to be pushed to the user terminal is determined according to feature information of a user and feature information of a video in the storage system corresponding to the server. Accordingly, the server can continuously push videos that meet the user's needs to the user terminal while the user is using the user page, thereby improving the user experience.