Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: The invention is directed to methods involved in the production of flavonoids, anthocyanins and other organic compounds. The invention provides cells engineered for the production of flavonoids, anthocyanins and other organic compounds, where the engineered cells include one or more genetic modifications that increase flavonoid production by increasing metabolic flux to flavonoid precursors and/or reducing carbon losses resulting from the production of byproducts.

Abstract: A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

Abstract: A method for micro-ridge mixed-sowing cultivation of rice includes: S1: draining away water at the maturity stage of the preceding crop until reaching a state allowing a harvester to operate; S2: harvesting the preceding crop, leaving the stubble, smashing the stalks of the preceding crop, and then spreading the smashed stalks on the stubble to form a rhizosphere layer for rice growth; S3: trenching the field to form ecological trenches; S4: flattening the standing stubble and the smashed stalks on the seedbed surface to form an underlying surface, molding seed-fertilizer-soil compounds into a ridge shape and fall the seed-fertilizer-soil compounds on the underlying surface to form ecological ridges, wherein a plurality of ecological ridges are formed between adjacent ecological trenches, and the seed-fertilizer-soil compounds are obtained by thoroughly mixing rice seeds, chemical fertilizers and soil at a mass ratio of 6 to 14:50 to 70:6,000 to 10,000.

Abstract: A method for micro-ridge mixed-sowing cultivation of dryland crops includes the following steps: S1: cleaning ditches and draining away water in dryland; S2: harvesting the preceding crop, leaving the stubble, smashing the stalks of the preceding crop, and then spreading the smashed stalks on the stubble; S3: trenching the dryland to form ecological trenches; S4: flattening the standing stubble and the smashed stalks on the seedbed surface to form an underlying surface, molding seed-fertilizer-soil compounds into a ridge shape and falling the seed-fertilizer-soil compounds on the underlying surface to form ecological ridges, wherein a plurality of ecological ridges are formed between adjacent ecological trenches, an ecological depression is formed between adjacent ecological ridges, and after sowing, an irrigation is carried out once in a manner, wherein the manner includes: draining water shortly after the irrigation, without leaving a water layer in the field.

Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell.

Abstract: A compound with anti-drug resistant bacteria activity having the following formula (I): is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

Abstract: A compound with anti-drug resistant bacteria activity having the following formula (I): (I) is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

Abstract: A compound with anti-drug resistant bacteria activity having the following formula (I): is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

Abstract: A compound with anti-drug resistant bacteria activity having the following formula (I): is disclosed. A method of preparing the compound of formula (I) is also disclosed.

Abstract: Described herein are non-natural olivetol synthase (OLS) variants, nucleic acids, engineered cells, method s for preparing cannabinoids, and compositions thereof. The non-natural olivetol OLS variants form desired cannabinoid precursor and products at increased rates, have higher affinity for pathway substrates, and/or byproducts are formed in lower amounts in their presence, as compared to wild type OLS. The OLS variants can be used to form linear polyketides, and can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

Abstract: A Syringa microphylla seed extract includes the following components by weight based on a total weight of the Syringa microphylla seed extract: 0.4944-0.7142 mg/g of echinacoside, 6.624-7.617 mg/g of oleuropein, 0.4276-0.6309 mg/g of verbascoside, 3.927-4.684 mg/g of syringin, and 4.505-5.250 mg/g of forsythiaside B. A method of preparing the Syringa microphylla seed extract is disclosed. A composition for treating antibiotic-resistant infections that includes the Syringa microphylla seed extract is also disclosed.

Abstract: This application relates to compounds of formulae (I) and (II) and compositions thereof useful as inhibitors of PIM kinases. Also provided are methods of synthesis and methods of use of PIM inhibitors in treating individuals suffering from cancerous malignancies.

Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing synthesis of olivetolic acid, olivetolic acid derivatives and/or cannabinoids.

Abstract: Systems and methods for determining collusion in student academic exams are described. One embodiment includes receiving an input electronic file with academic test data that includes student identifiers, question scores associated with each student identifier, and the total score for each student, calculating an identity score for each pair of students, determining a maximum identity score for each student, generating a normalized collusion score for each student by subtracting from the maximum identity score an average identity score to generate an identity metric, and dividing the identity metric by a local average identity metric to generate a normalized collusion score, where the local average identity metric is an estimated average of a set of identity metrics for a subset of students adjacent to one another on the total test score ranked list, writing student identifiers and normalized collusion scores associated with student identifiers to an output electronic file.

Abstract: A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

Abstract: A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

Abstract: A portable electronic device may include a housing, a display at least partially within the housing, a transparent cover over the display, and a battery at least partially within the housing. The battery may include a battery cell, a pouch encasing the battery cell, and a gas release relief system including a gas-permeable membrane configured to prevent liquid from escaping the pouch and a valve configured to selectively release gas from the pouch. The device may also include a processing system configured to, in a first mode of operation, cause the valve to open to allow gas to be released from the pouch, and, in a second mode of operation, cause the valve to close.

Abstract: Embodiments of the present invention provide an apparatus and methods for depositing a dielectric material using RF bias pulses along with remote plasma source deposition for manufacturing semiconductor devices, particularly for filling openings with high aspect ratios in semiconductor applications. In one embodiment, a method of depositing a dielectric material includes providing a gas mixture into a processing chamber having a substrate disposed therein, forming a remote plasma in a remote plasma source and delivering the remote plasma to an interior processing region defined in the processing chamber, applying a RF bias power to the processing chamber in pulsed mode, and forming a dielectric material in an opening defined in a material layer disposed on the substrate in the presence of the gas mixture and the remote plasma.