Abstract: A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives.

Abstract: An immunochromatography-assisted device is disclosed which facilitates accurate and rapid detection of an antigen contained in a fluid sample and from which background coloring and blank coloring possibly causing a malfunction at detection are successfully eliminated. The immunochromatography-assisted device comprises a porous carrier having an additive-impregnated part between a sample introducing part and a determining part, the additive-impregnated part carrying at least one selected from the group consisting of a surfactant, a water-soluble ammonium salt and a pH buffer such that it dissolves in a sample introduced into the carrier in order to move with the movement of the sample through the carrier. The porous carrier also has a labelled part segmented into plural zones.

Abstract: A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described.

Abstract: An apparatus and a method for transfusing liquid specimen for an optical characteristic measurement, which is excellent in operability and handling, and capable of introducing a liquid specimen into a sample cell without detaching the cell from an optical characteristic measurement apparatus and without bubbling, as well as a polarimeter are disclosed. According to the present invention, the specimen can be introduced into the sample cell without detaching the sample cell from the equipment, in a way of connecting the bottom of the sample cell with a reservoir for temporarily accommodating the liquid specimen through a tubular path, then varying a difference in the levels of the sample cell and the reservoir, or a way of using a syringe arranged in the tubular path. At expelling the specimen from the sample cell, the specimen is transfused into the reservoir in a similar manner.

Abstract: A sample cell for polarimetry is disclosed. It comprises: a base member having a tubular cavity for holding a specimen and for permitting a light to transmit therethrough, and a pair of light-transmitting windows for sealing a pair of open ends of the cavity; and a coil arranged around the base member for generating a magnetic field inside the cavity along an axial direction of the cavity. The specimen is supplied to the cavity through a channel for communicating the cavity with the outside.

Abstract: It is intended to present a sensor capable of labeling with more dyes, applying the labeled protein in immunochromatography making use of antigen-antibody reaction, and having an excellent sensitivity. In a buffer, a protein and a first covalent bonding compound that can react with this protein are reacted to prepare a protein conjugate, then a cyanine labeling dye is added in the buffer containing the protein conjugate, and the protein conjugate and cyanine labeling dye are reacted to prepare a dye labeled protein conjugate. Alternatively, in a buffer solution, a protein and a cyanine labeling dye are reacted to prepare a dye labeled protein, then a first covalent bonding compound that can react with the protein is added in the buffer containing the dye labeled protein, and the dye labeled protein and first covalent bonding protein are reacted to prepare a dye labeled protein conjugate.

Abstract: The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I . The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens.

Abstract: A labelling color for detecting cocaine of Formula (I) ##STR1## wherein X represents a halogen, and a method of detecting methamphetamine, including the steps of adding a labelling color of Formula (II) ##STR2## wherein X represents an anion to a first solution of methamphetamine antibody to form a labelling color-methamphetamine antibody complex, measuring the fluorescence of the first solution, adding a sample containing an unknown amount of methamphetamine to the first solution to form a second solution, measuring the fluorescence of the second solution, and correlating any change in the fluorescence between the first and second solutions to the concentration of methamphetamine in the sample.

Abstract: A labelling color which includes a pentamethine of the following formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.

Abstract: An immunofluorescent assay method, characterized in using an immunodetective reagent, which is an antigen-like substance comprising a fluorescent substance and an antigen chemically bonded thereto with or without intervention of an additional chemical bond, said fluorescent substance being changed in the wavelength or intensity of the fluorescence when an antibody comes close thereto, and said antigen being specific to said antibody.

Abstract: A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a higher affinity to methamphetamine that to the hapten N-(4-aminobutyl)amphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepeared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.

Abstract: A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.

Abstract: A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a high affinity to methamphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.

Abstract: An optical recording medium which comprises a substrate and a recording layer of at least two J-aggregates of dyes formed on the substrate is described. The dyes may be photochromic dyes or other types of dyes capable of forming the aggregates which have narrower absorption spectral ranges than original dyes. A plurality of information bits can be written in the recording layer by irradiation of one laser beam spot. The recording layer is formed by an LB film method or a method capable of fluidizing a dye solution in a direction horizontal to the substrate surface. A recording method using the medium is also described.

Abstract: An optical recording medium is prepared by the use of spiropyrane whose molecular structure contains an alkyl chain having at least 10 more carbon atoms, and by the use of an LB film method or a spin coating method. This recording medium is of a structure wherein when irradiated with the UV, the spiropyrane is photo-isomerized to photomerocyane and, thereafter, when subsequently heated at 35.degree. to 40.degree. C. for 15 minutes, a J-aggregate of the photomerocyane is formed in very stabilized form. When the recording medium is irradiated with light of wavelength of visible spectrum, the color is bleached with the information consequently recorded. The optical recording medium herein disclosed is rewritable and can retain the recorded information for 5,000 hours or more.

Abstract: A optical recording medium which comprises a substrate and a recording layer of at least two J-aggregates of dyes formed on the substrate is described. The dyes may be photochromic dyes or other types of dyes capable of forming the aggregates which have narrower absorption spectral ranges than original dyes. A plurality of information bits can be written in the recording layer by irradiation of one laser beam spot. The recording layer is formed by an LB film method or a method capable of fluidizing a dye solution in a direction horizontal to the substrate surface. A recording method using the medium is also described.

Abstract: An optical recording medium comprising a substrate and an information recording layer which consists of monolayer assemblies comprising a photochromic compound of which is based on cis-trans isomerization such as an amphipathic derivative of azobenzene, indigo or thioindigo having a long chain substituent, which monolayers are formed on the substrate by the Langmuir-Blodgett method and oriented in a two-dimensional plain. When the recording medium is irradiated by light, light absorption spectrum of the recording layer changes so that information is recorded. Since the photochromic compound is oriented in a monolayer, its photochromism lasts long.