Abstract: A nucleic acid sequence, the rainbow trout interferon regulatory factor-1 (IRF1A) promoter, is disclosed. This promoter is capable of expressing a nucleic acid sequence operably linked to it in fish cells. IRF1A can be operably linked to antigenic sequences for fish or shellfish pathogens, thus inducing an immune response in a fish transformed with such a nucleic acid. Some of the vectors described utilize a nucleic acid containing an inducible promoter operably linked to a nucleic acid sequence encoding a polypeptide capable of inducing programmed cell death (PCD).

Abstract: Subunit vaccines and their use for immunizing fish against infection by viruses are disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coli cells are transformed by pG8, whereby pure viral antigen is produced to provide a vaccine for the control of IHNV in fish.

Abstract: A vaccine for simultaneously immunizing large numbers of fish against infection by InfecThis invention was made with government support under the following grant numbers awarded by the U.S. Department of Commerce, National Oceanic and Atmospheric Administration, Science and Education Administration: NA81AA-D-00086 (1982-85, Recombinant DNA Technology in Aquaculture, Project No. R/FSD-9, Oregon State University); NA85AA-D-SG-095 (1985-87, Development of a Subunit Vaccine for IPNV, Project No. R/FSD-11, Oregon State University); and NA85AA-D-SG-095 (1987-89, Control of Virus Diseases in Fish, Project No. R/FSD-13, Oregon State University). The government has certain rights in this invention.