Abstract: The invention provides materials and methods for detecting the expression of genes encoding cytochrome p450, nuclear X receptors, phase H transferases, and solute carrier family uptake pumps. The materials include sets of primers, PCR amplicons and arrays. The methods of the invention include hybridization assays. Kits and assays for the detection of the expression of the genes are also provided by the invention. In addition, the invention provides the use of the materials and methods of the invention in drug screening assays.

Abstract: The disclosure describes materials and methods for detecting the expression of genes and generating a gene expression profile from drug-treated rat primary cells or established rat cell lines using a unique combination of rat cytochrome p450 enzyme, nuclear xenobiotic receptor, transferase and transporter gene sequences. The materials include sets of primers, PCR amplicons and arrays. The methods include hybridization assays. Assays for the detection of the expression of the genes are also provided. In addition, the disclosure provides the use of the materials and methods in drug screening assays and, specifically, the detection of potential drug-drug interaction(s).