Patents by Inventor Joe Chappell
Joe Chappell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11453884Abstract: A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.Type: GrantFiled: August 11, 2020Date of Patent: September 27, 2022Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATIONInventors: Joe Chappell, Xun Zhuang, Shuiqin Wu
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Patent number: 11149282Abstract: A system and method is provided for producing linear and branched hydrocarbons and in particular triterpenes in plant cells. The system and method includes a transformed plant cell with an isolated nucleic acid encoding a farnesyl diphosphate synthase (FPS) and with an isolated nucleic acid encoding a triterpene synthase.Type: GrantFiled: October 3, 2012Date of Patent: October 19, 2021Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATIONInventors: Joe Chappell, Shuiqin Wu
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Publication number: 20210071185Abstract: A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.Type: ApplicationFiled: August 11, 2020Publication date: March 11, 2021Inventors: Joe CHAPPELL, Xun ZHUANG, Shuiqin WU
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Patent number: 10738328Abstract: A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.Type: GrantFiled: November 27, 2013Date of Patent: August 11, 2020Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATIONInventors: Joe Chappell, Xun Zhuang, Wu Shuiqin
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Patent number: 10597665Abstract: A method is provided for modifying yeast to express mutant avian farnesyl disphospate synthase and the resulting modified yeast. The yeast advantageously includes additional mutants including but not limited to having ergosterol dependent growth and being erg-. The modified yeast are beneficial for the production of various terpenes including diterpenes.Type: GrantFiled: November 27, 2013Date of Patent: March 24, 2020Assignee: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATIONInventors: Joe Chappell, Xun Zhuang, Shuiqin Wu
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Patent number: 9821037Abstract: A compound is provided which comprises at least a portion of an amino acid linker-domain from squalene synthase. In alternative forms, the compound can include the amino-acid linker-domain from various fungus, including S. cerevisiae or the compound can be the functional equivalent and/or mimics an amino acid linker-domain from squalene synthase. A pharmaceutical composition includes the compound and may further include a pharmaceutical carrier. A method is provided for treating or controlling cholesterol metabolism and ergosterol metabolism in non-fungal organisms. One method includes a therapeutic treatment in humans by administering a therapeutically effective amount of the compound or pharmaceutical composition, to a patient in need of treatment, therefrom.Type: GrantFiled: April 21, 2014Date of Patent: November 21, 2017Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Tom D. Niehaus, Kristin Linscott
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Patent number: 9487762Abstract: A method and system are provided for the production of triterpene including methylated triterpenes. The method and system include isolated nucleic acid sequences encoding triterpene methyltransferases such as triterpene methyltransferases 1, 2, 3. Advantageously, the method and system includes transgenic plant cells via an expression vector for triterpene methyltransferase and optionally various triterpene synthase and prenyltransferase all with tags directing these enzymes to the chloroplast of the transgenic plant cells for using the chloroplast methyl erythritol phosphate (MEP) pathway in the triterpene biogenesis.Type: GrantFiled: October 9, 2013Date of Patent: November 8, 2016Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David S. Watt
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Patent number: 8822187Abstract: This application relates to the polypeptides, nucleic acid molecules, vectors, transfected cells, and methods for synthesis of triterpenes, including botryococcene.Type: GrantFiled: April 27, 2012Date of Patent: September 2, 2014Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David S. Watt
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Patent number: 8592180Abstract: This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.Type: GrantFiled: July 19, 2011Date of Patent: November 26, 2013Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Shigeru Okada, Tom Niehaus, Tim Devarenne
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Publication number: 20110294182Abstract: This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.Type: ApplicationFiled: July 19, 2011Publication date: December 1, 2011Inventors: Joe Chappell, Shigeru Okada, Tom Niehaus, Tim Devarenne
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Patent number: 8017835Abstract: The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.Type: GrantFiled: April 18, 2006Date of Patent: September 13, 2011Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Shuiqin Wu, Michel Schalk, Athony Clark
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Patent number: 7985568Abstract: This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.Type: GrantFiled: August 11, 2009Date of Patent: July 26, 2011Assignee: University of Kentucky Research FoundationInventors: Joe Chappell, Shigeru Okada, Tom Niehaus, Tim Devarenne
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Publication number: 20100041120Abstract: This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.Type: ApplicationFiled: August 11, 2009Publication date: February 18, 2010Applicant: University of Kentucky Research FoundationInventors: Joe CHAPPELL, Shigeru Okada, Tom Niehaus, Tim Devarenne
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Publication number: 20090123984Abstract: The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.Type: ApplicationFiled: April 18, 2006Publication date: May 14, 2009Applicant: The University of Kentucky Research FoundationInventors: Joe Chappell, Shuiqin Wu, Michel Schalk, Athony Clark
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Patent number: 7442785Abstract: The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.Type: GrantFiled: July 26, 2004Date of Patent: October 28, 2008Assignee: The University of Kentucky Research FoundationInventors: Joe Chappell, Brian Greenhagen
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Publication number: 20080201796Abstract: The present invention relates to plant expressing transgenes and to plants transformed to comprise additional copies of genes, said genes encoding at least a HMGR-CoA reductase and a terpene synthase. The invention further claims methods for preparing the plants, and a method for producing terpenes. The present thus provides a reliable and cost effective platform for generating any terpene, in particular any mono- and/or sesquiterpene of interest. For example, the skilled person may use any gene encoding a sesquiterpene synthase for accumulating the respective sesquiterpene in the plant of the present invention.Type: ApplicationFiled: June 26, 2006Publication date: August 21, 2008Applicant: University of Kentucky Research FoundationInventors: Joe Chappell, Shuiqin Wu, Michel Schalk, Athony Clark
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Publication number: 20060218661Abstract: The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.Type: ApplicationFiled: July 26, 2004Publication date: September 28, 2006Inventors: Joe Chappell, Bryan Greenhagen