Patents by Inventor Joe W. Gray
Joe W. Gray has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7094534Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: July 24, 2001Date of Patent: August 22, 2006Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Ollie-Pekka Kallioniemi, Frederic Waldman, Masaru Sakamoto
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Patent number: 7049424Abstract: The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.Type: GrantFiled: October 16, 1996Date of Patent: May 23, 2006Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Colin Collins, Soo-in Hwang, Tony Godfrey, David Kowbel, Johanna Rommens
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Patent number: 6989237Abstract: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention.Type: GrantFiled: October 6, 2004Date of Patent: January 24, 2006Assignee: The Regents of the University of CaliforniaInventors: Mack J. Fulwyler, Joe W. Gray
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Patent number: 6872817Abstract: Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogeneous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.Type: GrantFiled: June 5, 1995Date of Patent: March 29, 2005Assignee: The Regents of the Univ. of CaliforniaInventors: Joe W. Gray, Daniel Pinkel
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Patent number: 6855538Abstract: This invention provides improved components (e.g. array “pins”, print head, substrate platen, print head platen, and the like) for microarray printing devices as well as microarray printing devices incorporating such components. In one embodiment, this invention provides a microarray print head comprising a plurality of glass or quartz spotting capillaries disposed in a support that maintains a fixed spacing between the spotting capillaries and that permits the spotting capillaries to move in a direction parallel to the long axis of the capillaries.Type: GrantFiled: June 27, 2001Date of Patent: February 15, 2005Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Donna G. Albertson, Joe W. Gray, Greg Hamilton, Nils W. Brown, Steven M. Clark
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Publication number: 20040235039Abstract: Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.Type: ApplicationFiled: June 9, 2004Publication date: November 25, 2004Applicant: Regents of the University of CaliforniaInventors: Joe W. Gray, Daniel Pinkel, Douglas Tkachuk
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Patent number: 6818184Abstract: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention.Type: GrantFiled: April 17, 2003Date of Patent: November 16, 2004Assignee: The Regents of the University of CaliforniaInventors: Mack J. Fulwyler, Joe W. Gray
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Patent number: 6808878Abstract: The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.Type: GrantFiled: July 15, 1997Date of Patent: October 26, 2004Assignees: The Regents of the University of California, The Hospital for Sick ChildrenInventors: Joe W. Gray, Colin Conrad Collins, Soo-in Hwang, Tony Godfrey, David Kowbel, Johanna Rommens
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Patent number: 6785614Abstract: The present invention provides a novel method to identify rearrangements in a test genome, e.g., a tumor genome, when compared to a reference genome. This method provides major improvements over previous methods in terms of efficiency, rapidity, and cost-effectiveness. Briefly, this method involves generating or obtaining a large insert vector library from a test genome, sequencing the ends of the inserts in the library, and comparing the co-linearity of the sequenced ends in the library with corresponding sequences within a substantially-sequenced reference genome. This invention is useful for any of a number of applications, including for identifying rearrangements in tumor genomes and for determining genetic differences between closely related species as well as between different strains of the same species.Type: GrantFiled: May 31, 2000Date of Patent: August 31, 2004Assignee: The Regents of the University of CaliforniaInventors: Colin Collins, Stanislav Volik, Joe W. Gray
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Publication number: 20040096872Abstract: Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML) and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.Type: ApplicationFiled: June 30, 2003Publication date: May 20, 2004Inventors: Joe W. Gray, Daniel Pinkel, Douglas Tkachuk
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Publication number: 20040071603Abstract: This invention provides improved components (e.g. array “pins”, print head, substrate platen, print head platen, and the like) for microarray printing devices as well as microarray printing devices incorporating such components. In one embodiment, this invention provides a microarray print head comprising a plurality of glass or quartz spotting capillaries disposed in a support that maintains a fixed spacing between the spotting capillaries and that permits the spotting capillaries to move in a direction parallel to the long axis of the capillaries.Type: ApplicationFiled: June 6, 2003Publication date: April 15, 2004Applicant: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Donna G. Albertson, Joe W. Gray, Greg Hamilton, Nils W. Brown, Steven M. Clark, John Hanson
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Publication number: 20040038322Abstract: This invention provides methods, reagents and kits for treating cancer in a patient or subject, e.g., a human. Accordingly, the present methods can be used to monitor the efficacy of a cancer treatment and to treat cancer, e.g., by inhibiting the expression and/or activity of ZNF217 in a neoplastic cell.Type: ApplicationFiled: January 22, 2003Publication date: February 26, 2004Applicant: REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Colin Collins, Guiqing Huang, Joe W. Gray
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Patent number: 6664057Abstract: This invention pertains to the field of cancer genetics and cytogenetics. In particular, this invention pertains to the identification of a novel amplicon on human chromosome 20 which is associated with cancer. More particularly this invention pertains to the identification of a novel “amplicon” or genomic nucleic acid in a region of amplification at about 20q13.2 which has been associated with a variety of cancers, particularly breast cancer. The novel amplicon of the invention can be used as a probe specific for this region of 20q13.2 as well as for the diagnosis and prognosis of various cancers. Also provided are kits for screening for the presence and copy number of the novel amplicon of the invention in a sample containing human nucleic acid.Type: GrantFiled: June 28, 2001Date of Patent: December 16, 2003Assignee: The Regents of the University of CaliforniaInventors: Donna G. Albertson, Daniel Pinkel, Colin Conrad Collins, Joe W. Gray
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Publication number: 20030207308Abstract: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention.Type: ApplicationFiled: April 17, 2003Publication date: November 6, 2003Applicant: The Regents of the University of CaliforniaInventors: Mack J. Fulwyler, Joe W. Gray
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Patent number: 6610499Abstract: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention.Type: GrantFiled: August 31, 2000Date of Patent: August 26, 2003Assignee: The Regents of the University of CaliforniaInventors: Mack J. Fulwyler, Joe W. Gray
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Patent number: 6607877Abstract: Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome.Type: GrantFiled: June 7, 1995Date of Patent: August 19, 2003Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Daniel Pinkel
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Publication number: 20030148270Abstract: The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases.Type: ApplicationFiled: October 16, 1996Publication date: August 7, 2003Inventors: JOE W. GRAY, COLIN COLLINS, SOO-IN HWANG, TONY GODFREY, DAVID KOWBEL, JOHANNA ROMMENS
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Patent number: 6596479Abstract: Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.Type: GrantFiled: June 5, 1995Date of Patent: July 22, 2003Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Daniel Pinkel
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Publication number: 20030108943Abstract: Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.Type: ApplicationFiled: December 30, 2002Publication date: June 12, 2003Inventors: Joe W. Gray, Daniel Pinkel
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Patent number: 6562565Abstract: The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.Type: GrantFiled: June 26, 1996Date of Patent: May 13, 2003Assignees: The Regents of the University of California, Medical Research CouncilInventors: Daniel Pinkel, Donna Albertson, Joe W. Gray