Abstract: The present invention provides compositions comprising oligonucleotides that have 3? end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3? end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.

Abstract: The present invention provides compositions comprising oligonucleotides that have 3? end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3? end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.

Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.

Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.

Abstract: The present invention relates to reactions conducted on or in solid surface materials, including liquid phase reactions within capillary tubes and arrays of capillary tubes or open end reaction microchambers. A wide array of biomolecular and combinatorial synthesis reactions may be used with the present invention. For example, the present invention provides methods for forming and cleaving nucleic acid cleavage structures on or in solid surface materials.

Abstract: The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.

Abstract: The invention relates to oligonucleotide probes attached to discrete particles wherein the particles can be grouped into a plurality of sets based on a physical property. A different probe is attached to the discrete particles of each set, and the identity of the probe is determined by identifying the discrete particles from their physical property. The physical property includes any that can be used to differentiate the discrete particles, and includes, for example, relative or absolute location, size, flourescence, radioactivity, electromagnetic charge, or absorbance, or label(s) may be attached to the particle such as a dye, a radionuclide, or an EML. The invention also relates to methods using the probes complexed with the discrete particles to analyze target nucleic acids.

Abstract: The present invention relates to spatially-addressable sandwich arrays of compounds, particularly biological compounds such as peptides and polynucleotide probes, and methods of making and using the same. The present invention also relates to a method and device for holding together the substrates of the sandwich array, more particularly, a clamping device for securely yet safely holding substrates of a sandwich array together during assembly, use, storage, and/or transport of the sandwich array.

Abstract: The present device is directed to a reagent transfer device for transferring a plurality of reagent samples from one location to another. A transfer plate has a top surface with reservoirs, a bottom surface with orifices, and channels connecting the reservoirs and orifices. Fluid is moved from the reservoirs, through the channels and out of the orifices to form samples on the bottom surface about the orifices. A movement apparatus is provided for bringing the samples into contact with deposit surfaces at various deposition positions. Arrays of at least 100 orifices per square centimeter are provided.

Abstract: The present invention relates to methods of using labeled tracers to generate spatially addressable arrays of immobilized molecules, particularly polynucleotides, that can be normalized for differences in immobilization efficiencies at different addresses in the array. It also relates to the arrays generated by the method and to use of these arrays to enhance discrimination in array-based assays, particularly the discrimination between perfectly matched hybrids and hybrids containing a single mismatch in nucleic acid hybridization assays.

Abstract: The present device is directed to a reagent transfer device for transferring a plurality of reagent samples from one location to another location having a deposit surface. The device includes a reagent tray having a top surface with a top surface area and a plurality of wells in the ratio of at least 1,000 wells per square centimeter of top surface area, and a transfer member having a transfer surface with a plurality of pins extending therefrom. Each of the pins is positioned to correspond to one of the wells so that when the transfer member is moved in the Z-direction the pins are simultaneously dipped into the corresponding wells. The device further includes a mechanism for moving the transfer member between a first position in which reagent from the wells is deposited onto the corresponding pins and a second position in which reagent on the pins is deposited on the deposit surface. The mechanism for moving is adapted to move the transfer member in the X-direction, the Y-direction and the Z-direction.