Patents by Inventor Johan Ohman
Johan Ohman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240132538Abstract: The present invention relates to protein purification, primarily in the chromatographic field. More closely, the invention relates to affinity chromatography using a split intein system with an improved C-intein tag and N-intein ligand, wherein the target protein may be purified as a tag-less end product with a native N-terminus.Type: ApplicationFiled: November 20, 2020Publication date: April 25, 2024Inventors: Christopher James Sevinsky, Peter Lundback, Johan Ohman, Gregory Grossmann, Sean R. Dinn
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Patent number: 11958049Abstract: A sample loading cartridge (1) for a microfluidic device comprises a cartridge body (10) with a sample reservoir (20) configured to house a volume of a liquid sample (3) and a sample port (30) in connection with the sample reservoir (20). The cartridge (1) also comprises an output channel (40) extending from the sample reservoir (20) and a feedback channel (50) connected to the sample reservoir (20) and to the sample port (30). The cartridge body (10) comprises a detection portion (60) aligned with the feedback channel (50) to enable detection of any sample (3) in the feedback channel (50). The flow resistance of the feedback channel (50) is lower than the flow resistance of the output channel (40) to cause liquid sample (3) received in the sample port (30) to enter the feedback channel (50) with substantially no liquid sample (3) entering the output channel (40).Type: GrantFiled: September 20, 2019Date of Patent: April 16, 2024Assignee: ASTREGO DIAGNOSTICS ABInventors: Johan Öhman, Martin Lovmar, Lovisa Söderberg, Mikael Olsson
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Patent number: 11946029Abstract: A microfluidic device (1) comprises a substrate (10) having a flow input channel (30) in fluid connection with a first fluid port (31) and a flow output channel (40) in fluid connection with a third fluid port (41) and cell channels (20) disposed between the flow input channel (30) and the flow output channel (40). The cell channels (20) comprise a respective obstruction (25) designed to prevent the target cells from passing the respective obstruction (25) and into the flow output channel (40). The microfluidic device (1) also comprises a pre-filter (50) with a filter channel (60) in fluid connection with a first filter port (61) and pre-filter channels (70) adapted to accommodate the target cells. A respective first end (72) of the pre-filter channels (70) is in fluid connection with the filter channel (60) and a respective second end (74) of the pre-filter channels (70) is in fluid connection with the flow input channel (30).Type: GrantFiled: February 15, 2019Date of Patent: April 2, 2024Assignee: ASTREGO DIAGNOSTICS ABInventors: Özden Baltekin, Ove Öhman, Martin Lovmar, Johan Elf, Mikael Olsson
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Publication number: 20230278035Abstract: A cartridge comprises a chip chamber configured to house a microfluidic chip comprising a plurality of sets of cell channels configured to capture cells from a biological sample. The cartridge also comprises a sample chamber configured to receive the biological sample and be in fluid connection with the plurality of sets of cell channels and a plurality of medium reservoirs. Each medium reservoir of the plurality of medium reservoirs is configured to be in fluid connection with a respective set of cell channels of the plurality of sets of cell channels. The cartridge further comprises culture medium source in fluid connection with the plurality of medium reservoirs and configured to supply a culture medium to the plurality of medium reservoirs.Type: ApplicationFiled: February 22, 2023Publication date: September 7, 2023Inventors: Robert FREDRIKSSON, Mikael Olsson, Johan ÖHMAN, Lovisa SÖDERBERG, Özden BALTEKIN
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Publication number: 20220032302Abstract: A sample loading cartridge (1) for a microfluidic device comprises a cartridge body (10) with a sample reservoir (20) configured to house a volume of a liquid sample (3) and a sample port (30) in connection with the sample reservoir (20). The cartridge (1) also comprises an output channel (40) extending from the sample reservoir (20) and a feedback channel (50) connected to the sample reservoir (20) and to the sample port (30). The cartridge body (10) comprises a detection portion (60) aligned with the feedback channel (50) to enable detection of any sample (3) in the feedback channel (50). The flow resistance of the feedback channel (50) is lower than the flow resistance of the output channel (40) to cause liquid sample (3) received in the sample port (30) to enter the feedback channel (50) with substantially no liquid sample (3) entering the output channel (40).Type: ApplicationFiled: September 20, 2019Publication date: February 3, 2022Inventors: Johan ÖHMAN, Martin LOVMAR, Lovisa SÖDERBERG, Mikael OLSSON
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Publication number: 20190263856Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.Type: ApplicationFiled: November 14, 2017Publication date: August 29, 2019Inventors: Johan Ohman, Mats Ander, Therese Graner, Anneli Jorsback, Jinyu Zou
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Patent number: 9983203Abstract: Provided are methods for protein analysis in which proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. The proteins to be analyzed may be provided as denatured protein samples in the presence of a denaturing agent.Type: GrantFiled: February 12, 2015Date of Patent: May 29, 2018Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Daniel Ivansson, Camilla Estmer Nilsson, Therese Graner, Johan Ohman
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Patent number: 9797871Abstract: The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about ?20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.Type: GrantFiled: April 28, 2011Date of Patent: October 24, 2017Assignee: GE Healthcare Bio-Sciences ABInventors: James Van Alstine, Johan Ohman, Philippe Busson, Ronnie Palmgren, Klas Allmer, John Daicic
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Publication number: 20160320377Abstract: The present invention relates to a method for protein analysis in which the proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection.Type: ApplicationFiled: February 12, 2015Publication date: November 3, 2016Inventors: Daniel Ivannson, Camilla Estmer-Nilsson, Therese Graner, Johan Ohman
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Patent number: 9340828Abstract: The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions <5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH >5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.Type: GrantFiled: October 25, 2012Date of Patent: May 17, 2016Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Camilla Estmer Nilsson, Johan Ohman
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Publication number: 20140272999Abstract: The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions <5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH >5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.Type: ApplicationFiled: October 25, 2012Publication date: September 18, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Camilla Estmer Nilsson, Johan Ohman
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Patent number: 8632883Abstract: The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules.Type: GrantFiled: February 12, 2009Date of Patent: January 21, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Anders Larsson, Ulrika Meyer, Nils Norrman, Johan Ohman
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Publication number: 20130131323Abstract: The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about ?20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.Type: ApplicationFiled: April 28, 2011Publication date: May 23, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Johan Ohman, Philippe Busson, Ronnie Palmgren, Klas Allmer, John Daicic
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Publication number: 20100294977Abstract: The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules.Type: ApplicationFiled: February 12, 2009Publication date: November 25, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Anders Larsson, Ulrika Meyer, Nils Norrman, Johan Ohman
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Patent number: 6605706Abstract: Methods for producing correctly folded biological active recombinant protein or polypeptide comprise the steps of expressing the protein or polypeptide in prokaryotic cells, harvesting the cells, directly solubilizing the cells in a buffer at pH of about 8 to 11 with the chaotropic agent and a reducing agent, and diluting the solution with water and a diluent. The method is conducted in the absence of mechanical disruption of the cells and isolation and washing of refractile bodies.Type: GrantFiled: July 6, 1998Date of Patent: August 12, 2003Assignee: Pharmacia ABInventors: Jan-Gunnar Gustafsson, Johan Öhman
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Publication number: 20020198189Abstract: A method of treatment or prophylaxis of SSAO-mediated complications in mammals including humans, comprising administering to a patient in need of such treatment a therapeutically effective amount of a compound of Formula (I): 1Type: ApplicationFiled: November 9, 2001Publication date: December 26, 2002Inventors: Olivier Besencon, Rolf Olsson, Johan Ohman, Patrizia Caldirola