Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.

Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules using a rotation of a magnetic field to move streptavidin-labeled magnetic beads relative to a buffered liquid medium to increase the probability of capturing and preventing damage to a long polynucleotide comprising a DNA target segment. The methods may use a rotation of the magnetic field relative to the mixture or a rotation of the mixture relative to the magnetic field for causing magnetic bead rotation in the buffered liquid medium and increasing the binding of biotinylated nucleotides of an extended polynucleotide primer to streptavidin-labeled magnetic beads, and winding or condensation of a polynucleotide onto the streptavidin-labeled magnetic beads to prevent damage to the long polynucleotide comprising a DNA target segment.

Abstract: Composition and method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method includes: administering an aqueous formulation comprising magnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the magnetic particles; forming one or more aligned chains of the magnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Abstract: The invention generally relates to a method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method comprising the steps of: administering an aqueous formulation comprising superparamagnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the superparamagnetic particles; forming one or more aligned chains of the superparamagnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Abstract: The invention relates to rapid cryptography methods and devices for secure data access. A private key is temporarily created for a cryptography use on a computer for decrypting a document. A device comprising a second data matrix, which is external to the computer, is read by the computer, which processes the second data matrix using a first data matrix already present on the computer. Then, the first data matrix provides the means for stepping through the second data matrix to create a private key. Next, the private key is erased immediately after the private key is created and used in a cryptography process for accessing secure data. The first and second data matrices are hidden by the user, and the private key is not stored, but instead is temporarily re-created as needed only for rapidly accessing secure data.

Abstract: The invention generally relates to a method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method comprising the steps of: administering an aqueous formulation comprising superparamagnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the superparamagnetic particles; forming one or more aligned chains of the superparamagnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.

Abstract: The invention generally relates to a method of regenerating a nerve fiber in a damaged neural tissue of a patient, the method comprising the steps of: administering an aqueous formulation comprising superparamagnetic particles to the damaged neural tissue in the patient; applying a magnetic field in an orientation which is parallel to the nerve fiber; using the magnetic field for aligning the superparamagnetic particles; forming one or more aligned chains of the superparamagnetic particles in the magnetic field as a scaffold to guide directional growth of regenerating nerve cells; and reconnecting damaged nerve ends in the damaged neural tissue of the patient.

Abstract: An automated protein preparation technology that may use magnetic microparticles to isolate proteins in their native state from specific genomic loci of interest via the chromatin to which they are bound is described. After extraction, the targeted proteins may be purified for downstream analysis by quantitative mass spectrometry or ELISA. The identification of DNA-bound proteins, histones and their post-translational modification is of high scientific and pharmaceutical importance due to the role of DNA-binding proteins in the cauzation and development of human disease; in particular, cancer.

Abstract: An automated protein preparation technology that may use magnetic microparticles to isolate proteins in their native state from specific genomic loci of interest via the chromatin to which they are bound is described. After extraction, the targeted proteins may be purified for down-stream analysis by quantitative mass spectrometry or ELISA. The identification of DNA-bound proteins, histones and their post-translational modification is of high scientific and pharmaceutical importance due to the role of DNA-binding proteins in the causation and development of human disease; in particular, cancer.

Abstract: A minimally invasive method to eliminate circulating agents from an organism to prevent and treat diseases is disclosed. The method utilizes immunomagnetic methods to concentrate and localize disease-associated agents in a small region of the body. Subsequently, the complexes are removed from the body. Removal of disease-causing or disease-promoting agents (circulating tumor cells, bacteria, viruses and virus-infected cells, certain immune cells) would add a significant new option for intervention of disease progression and supplement other therapeutic options.

Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.

Abstract: The invention is directed to methods to identify the location in a genome of a nonfixed or multicopy genomic element using microarrays or sequencing.

Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.

Abstract: Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.

Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.

Abstract: A genetic analysis device particularly for determining the presence or absence of Single Nucleotide Polymorphisms (SNPs) within specific sequences of DNA. The device includes a housing, at least one glass slide member, and an elastomeric member with channels thereon. Oligo arrays are spotted on the glass slide member(s) and subjected to DNA samples, reagents or the like. A plurality of openings or ports allow entry of samples, reagents or wash materials, while a plurality of exit ports or openings allow removal of such materials. The assay devices can be used for multiple samples or a single sample. A plurality of synthesis devices can be positioned in a support base in order to allow sampling in an automated manner. The synthesis devices can be provided in a 96 well microtiter format.

Abstract: The present invention relates to microstructures which a fabricated from a cast or molded polymer material, such as polydimethylsiloxane (PDMS). The invention describes and claims methods and compositions from which a variety of microstructures may be prepared. The microstructures are particularly useful for use in assays and reactions in the relating to biological assays, including applications in medical diagnostics. Such assays and reactions included, but are by no means limited to, polynucleotide sequencing and polynucleotides analysis, including analysis of single nucleotide polymorphisms, and for polynucleotide amplification such as PCR. The microstructures of the invention may contain many features that are useful for such applications. For example, the microstructures may comprise wells or channels for biological fluids and/or reagents, as well as pumps and valves for directing such fluids, e.g., in a biological assay.

Abstract: A genetic analysis device particularly for determining the presence or absence of Single Nucleotide Polymorphisms (SNPs) within specific sequences of DNA. The device includes a housing, at least one glass slide member, and an elastomeric member with channels thereon. Oligo arrays are spotted on the glass slide member(s) and subjected to DNA samples, reagents or the like. A plurality of openings or ports allow entry of samples, reagents or wash materials, while a plurality of exit ports or openings allow removal of such materials. The assay devices can be used for multiple samples or a single sample. A plurality of synthesis devices can be positioned in a support base in order to allow sampling in an automated manner. The synthesis devices can be provided in a 96 well microtiter format.

Abstract: Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.