Abstract: A method for the detection and quantitation of analytes of interest and variants of the analyte of interest comprising the steps of: (i) providing a sample containing an analyte of interest; (ii) spiking sample with known amount of calibrant; (iii) performing an LCMS or LCMSMS analysis on the spiked sample to produce a data set; (iv) determining from said data set the relative quantity of analyte of interest to calibrant; (v) calculating the absolute quantity of the analyte of interest from said relative quantity of analyte of interest and said known amount of calibrant; (vi) searching for one or more previously identified candidate sequences for one or more known variants denoted by one or more specific peaks to identify the presence of said one or more known variants within the sample; (vii) determining the relative quantity or amount of said one or more variants to said analyte of interest; and (viii) calculating the absolute quantity or amount of any of said one or more variants present in the sample.

Abstract: This invention comprises a method of imaging of a substrate in which a sample of interest is first ionized at multiple known positions whereafter a mass spectrum of the ionized sample at each of the multiple known positions is produced using a Mass Spectrometer. An overall spectrum for the whole sample is then created, and a number of peaks within the overall spectrum is selected. A scan distribution for at least some of the selected peaks is created, and the scan distributions are compared to identify correlations between different analytes within the sample.

Abstract: This invention comprises a method of imaging of a substrate in which a sample of interest is first ionized at multiple known positions whereafter a mass spectrum of the ionized sample at each of the multiple known positions is produced using a Mass Spectrometer. An overall spectrum for the whole sample is then created, and a number of peaks within the overall spectrum is selected. A scan distribution for at least some of the selected peaks is created, and the scan distributions are compared to identify correlations between different analytes within the sample.

Abstract: A liquid chromatography micro-autosampler is disclosed that permits the sampling of very small samples quantities at high accuracy, while minimizing extracolumn band broadening. The autosampler employ a sample intake tubing connected to the injection valve, that is further connected to an aspirating device. The intake tubing terminates within the hollow bore of a needle which is used to penetrate sample containers. Improved injection profiles are obtained by minimizing injection plug dispersion with back flushing of the injection plug and a low dispersion injection routine and extremely small sample quantities can be handled with a micro pick-up injection routine.