Abstract: Provided is a surface having metal regions and an interstitial region having a composition that differs from the metal regions, wherein a continuous gel layer coats the surface across the metal regions and the interstitial regions. Nucleic acids or other analytes can be attached to the continuous gel layer such that a greater amount is attached over the metal regions than over the interstitial region. Also provided are methods for making such surfaces. Methods are also provided for making an array of nucleic acids or other analytes using such surfaces.

Abstract: In an illustrative embodiment, and to remove overall peak demand, a dealership energy management system may include bi-directional charging devices that may be associated with inventory vehicles and output devices that may be associated with customer vehicles. Inventory may be processed and associated with days stored at the dealership. The inventory vehicles may be charged up to 60% during off-peak hours to provide charge to incoming customer vehicles during peak hours. The inventory vehicles may discharge down to 40%, while providing charge to the customer vehicles, before another vehicle within the inventory is selected to provide charge. Inventory vehicles may be charged and discharged based on the days stored at the dealership as well as whether those vehicles have been discharged below a threshold.

Abstract: A fluidic device including an inorganic solid support attached to an organic solid support by a bonding layer, wherein the inorganic solid support has a rigid structure and wherein the bonding layer includes a material that absorbs radiation at a wavelength that is transmitted by the inorganic solid support or the organic solid support; and a channel formed by the inorganic solid support and the organic solid support, wherein the bonding layer that attaches the inorganic solid support to the organic solid support provides a seal against liquid flow. Methods for making fluidic devices, such as this, are also provided.

Abstract: Provided is a surface having metal regions and an interstitial region having a composition that differs from the metal regions, wherein a continuous gel layer coats the surface across the metal regions and the interstitial regions. Nucleic acids or other analytes can be attached to the continuous gel layer such that a greater amount is attached over the metal regions than over the interstitial region. Also provided are methods for making such surfaces. Methods are also provided for making an array of nucleic acids or other analytes using such surfaces.

Abstract: A method of registering features in a repeating pattern can include (a) providing an object having a repeating pattern of features and a fiducial; (b) obtaining a target image of the object, wherein the target image includes the repeating pattern of features and the fiducial; (c) comparing the fiducial in the target image to reference data, wherein the reference data includes xy coordinates for a virtual fiducial; and (d) determining locations for the features in the target image based on the comparison of the virtual fiducial in the reference data to the fiducial in the data from the target image. The fiducial can have at least concentric circles that produce three different signal levels. The locations of the features can be determined at a variance of less than 5 ?m.

Abstract: A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Abstract: In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

Abstract: In an illustrative embodiment, and to remove overall peak demand, a dealership energy management system may include bi-directional charging devices that may be associated with inventory vehicles and output devices that may be associated with customer vehicles. Inventory may be processed and associated with days stored at the dealership. The inventory vehicles may be charged up to 60% during off-peak hours to provide charge to incoming customer vehicles during peak hours. The inventory vehicles may discharge down to 40%, while providing charge to the customer vehicles, before another vehicle within the inventory is selected to provide charge. Inventory vehicles may be charged and discharged based on the days stored at the dealership as well as whether those vehicles have been discharged below a threshold.

Abstract: In an illustrative embodiment, and to remove overall peak demand, a dealership energy management system may include bi-directional charging devices that may be associated with inventory vehicles and output devices that may be associated with customer vehicles. Inventory may be processed and associated with days stored at the dealership. The inventory vehicles may be charged up to 60% during off-peak hours to provide charge to incoming customer vehicles during peak hours. The inventory vehicles may discharge down to 4%, while providing charge to the customer vehicles, before another vehicle within the inventory is selected to provide charge. Inventory vehicles may be charged and discharged based on the days stored at the dealership as well as whether those vehicles have been discharged below a threshold.

Abstract: In an illustrative embodiment, and to remove overall peak demand, a dealership energy management system may include bi-directional charging devices that may be associated with inventory vehicles and output devices that may be associated with customer vehicles. Inventory may be processed and associated with days stored at the dealership. The inventory vehicles may be charged up to 60% during off-peak hours to provide charge to incoming customer vehicles during peak hours. The inventory vehicles may discharge down to 40%, while providing charge to the customer vehicles, before another vehicle within the inventory is selected to provide charge. Inventory vehicles may be charged and discharged based on the days stored at the dealership as well as whether those vehicles have been discharged below a threshold.

Abstract: Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Abstract: Embodiments for registering features in a repeating pattern are described. These embodiments can include providing an object having a repeating pattern of features and a fiducial. These embodiment can also include obtaining a target image of the object, where the target image includes the repeating pattern of features and the fiducial. These embodiment can also include comparing the fiducial in the target image to reference data, where the reference data includes xy coordinates for a virtual fiducial. These embodiment can also include determining locations for the features in the target image based on the comparison of the virtual fiducial in the reference data to the fiducial in the data from the target image. The fiducial can have at least concentric circles that produce three different signal levels. The locations of the features can be determined at a variance of less than 5 ?m.

Abstract: A sensor includes two electrodes and a modulatable electrically conductive channel attached to the two electrodes. The modulatable electrically conductive channel includes a modified, partially double stranded nucleic acid polymer electrically connected to the two electrodes and bridging the space between the two electrodes. The modified, partially double stranded nucleic acid polymer includes two polynucleotide chains partially bonded together, a gap in a first of the polynucleotide chains wherein nucleotide bases are missing, and a plurality of nucleotide bases of a second of the polynucleotide chains exposed at the gap in the first of the polynucleotide chains.

Abstract: A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.

Abstract: A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.

Abstract: Example super-resolution microscopy systems are described herein that are configured for relatively high throughput. The disclosed microscopy systems can be to generate an array of sub-diffraction activated areas for imaging. The microscopy systems can be to utilize imaging techniques that employ time delay integration to build up super-resolution images over time. The disclosed microscopy systems can utilize long-lived fluorophores in conjunction with wide field and patterned illumination to generate super-resolution images of a sample with relatively high throughput.

Abstract: A photographic image exchange system includes an imaging device having an imager that performs imaging, a communicator that performs communication with a server, and a controller that controls the communicator such that an image that includes a registered target among images captured by the imager is transmitted to the server, and the server provides the image received from the imaging device to a device used by a user who is related to the registration.

Abstract: Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.