Patents by Inventor John Alfred Lewis

John Alfred Lewis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of poliovirus at high titers for vaccine production
    Patent number: 8546123
    Abstract: Provided is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.
    Type: Grant
    Filed: July 16, 2010
    Date of Patent: October 1, 2013
    Assignee: Crucell Holland B.V.
    Inventor: John Alfred Lewis
  • METHOD FOR THE PRODUCTION OF ADENOVIRAL VECTORS
    Publication number: 20110207202
    Abstract: The invention provides methods for large-scale production of recombinant adenovirus 35, using perfusion systems and infection at very high-cell densities.
    Type: Application
    Filed: October 29, 2009
    Publication date: August 25, 2011
    Inventors: Alfred Luitjens, John Alfred Lewis
  • Production of poliovirus at high titers for vaccine production
    Publication number: 20110027317
    Abstract: Provided is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.
    Type: Application
    Filed: July 16, 2010
    Publication date: February 3, 2011
    Inventor: John Alfred Lewis