Patents by Inventor John Andrew Sheridan
John Andrew Sheridan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11821032Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: August 21, 2020Date of Patent: November 21, 2023Assignee: IsoPlexis CorporationInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20230340583Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: April 6, 2023Publication date: October 26, 2023Inventors: Luisa ANDRUZZI, Jimmy ADEDIRAN, Timothy PELLETIER, Austin RICKER, Angela DELUCIA, Ben MCNALLY, Dona HEVRONI, Mong Sano MARMA, John Andrew SHERIDAN
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Publication number: 20220073976Abstract: Cyclone Rolling Circle Amplification (CRCA) based next-generation sequencing (NGS) using a multiple primer rolling circle amplification (RCA) reaction is generated using two or more tandem primers from the same strand on different locations of library adaptor regions of double stranded enriched targeted polymerase chain reaction (PCR) library products. This process allows multiple initiation and syntheses of the RCA reaction by an enzyme on the same circular template molecule, which is beneficial since the two or more primers complement each other in generating uniform amplification of the target circle population. Also, a method for keeping DNA nanoballs (also known as rolonies) compact, or more compact, and for pre-priming rolonies before sequencing to eliminate the hybridization of a seqeuncing primer after seeding the rolonies, and a Rolonies rolling circle amplification (RCA) based next-generation sequencing (NGS) using a dual Rolonies primer approach named REPLI-Rolony.Type: ApplicationFiled: September 10, 2020Publication date: March 10, 2022Inventors: Seiyu Hosono, Michel Georges Perbost, Ryan Steven Hindman, Austin Ricker, Robert Pinard, John Andrew Sheridan
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Publication number: 20210002720Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: August 21, 2020Publication date: January 7, 2021Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20200385801Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: ApplicationFiled: June 8, 2020Publication date: December 10, 2020Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
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Patent number: 10844430Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: January 24, 2018Date of Patent: November 24, 2020Assignee: Qiagen Sciences, LLCInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Publication number: 20190382835Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: ApplicationFiled: December 31, 2018Publication date: December 19, 2019Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
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Publication number: 20190226018Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: ApplicationFiled: January 24, 2018Publication date: July 25, 2019Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Patent number: 9901887Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: GrantFiled: September 30, 2016Date of Patent: February 27, 2018Assignee: Life Technologies CorporationInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech, John Andrew Sheridan
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Publication number: 20170275688Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: ApplicationFiled: January 3, 2017Publication date: September 28, 2017Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
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Publication number: 20170014784Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: ApplicationFiled: September 30, 2016Publication date: January 19, 2017Inventors: Jonathan SCHULTZ, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech, John Andrew Sheridan
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Patent number: 9458485Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: GrantFiled: July 14, 2015Date of Patent: October 4, 2016Assignee: Life Technologies CorporationInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Krishnan Thwar, Todd Roswech, John Andrew Sheridan
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Publication number: 20150314294Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: ApplicationFiled: July 14, 2015Publication date: November 5, 2015Inventors: Jonathan SCHULTZ, John Nobile, Brian Reed, Prasanna Krishnan Thwar, Todd Roswech, John Andrew Sheridan
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Patent number: 9150917Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: GrantFiled: October 18, 2013Date of Patent: October 6, 2015Assignee: Life Technologies CorporationInventors: Todd Rearick, Jessica Lynn Reed, Jason Gioia, Devin Dressman, Nicholas Hapshe, Brian Reed, John Andrew Sheridan
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Publication number: 20150031558Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: ApplicationFiled: October 15, 2014Publication date: January 29, 2015Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
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Publication number: 20140100122Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Ph.D., Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
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Publication number: 20110096620Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: ApplicationFiled: December 29, 2010Publication date: April 28, 2011Applicant: APPLIED BIOSYSTEMS, LLCInventors: Lev KOTLER, John Andrew Sheridan, Gina Costa, Joseph Podhasky
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Patent number: 7883265Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: GrantFiled: May 30, 2008Date of Patent: February 8, 2011Assignee: Applied Biosystems, LLCInventors: Lev Kotler, John Andrew Sheridan, Gina Costa, Joseph Podhasky
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Publication number: 20090036325Abstract: The present teachings relate to improved methods, kits, and compositions for making nucleic acid libraries and sequencing nucleic acids. In some embodiments, directionally defined concatamers are generated, facilitating sequencing efforts.Type: ApplicationFiled: May 21, 2008Publication date: February 5, 2009Applicant: Applera CorporationInventors: Kevin J. McKernan, John Andrew Sheridan, Douglas R. Smith
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Publication number: 20090035825Abstract: A vortex mixer and method for forming an emulsion wherein the mixer is adapted to form an emulsion with a desired droplet size and having a desired volume. The vortex mixer provides improved uniformity in emulsion preparation and may be used to create multiple emulsions simultaneously.Type: ApplicationFiled: May 30, 2008Publication date: February 5, 2009Applicant: Applera CorporationInventors: Lev Kotler, John Andrew Sheridan, Gina Costa, Joseph Podhasky