Patents by Inventor John Anthony Schiel

John Anthony Schiel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences
    Patent number: 11834670
    Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: December 5, 2023
    Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLC
    Inventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith
  • SITE-SPECIFIC DNA MODIFICATION USING A DONOR DNA REPAIR TEMPLATE HAVING TANDEM REPEAT SEQUENCES
    Publication number: 20180305718
    Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
    Type: Application
    Filed: April 19, 2017
    Publication date: October 25, 2018
    Inventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith