Patents by Inventor John Antonius Verhees
John Antonius Verhees has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10563223Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.Type: GrantFiled: September 29, 2017Date of Patent: February 18, 2020Assignee: CellaGenics B.V.Inventors: Arie Pieter Otte, Michel Siep, John Antonius Verhees, Femke Hoeksema, Henricus Johannes Maria Van Blokland
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Patent number: 10106799Abstract: The present invention relates to nucleic acid constructs comprising selectable marker genes in a multicistronic transcription unit for use in the generation and selection of eukaryotic host cells for expression of a gene product of interest. For increased stringency of selection, the coding sequence of the selectable marker may be directed preceded by a relatively short functional open reading frame to reduce the efficiency of translation of the selectable marker, and/or the amino acid sequence of the selectable marker may comprise one or more mutations that reduce the level of resistance provide by the mutated marker as compared to its wild type counterpart. The invention further relates to methods for generating eukaryotic host cells for expression of a gene product of interest, wherein these nucleic acid constructs are used, and to methods for producing a gene product of interest wherein thus generated host cells are applied.Type: GrantFiled: February 8, 2016Date of Patent: October 23, 2018Assignee: CELLAGENICS B.V.Inventors: Arie Pieter Otte, Henricus Johannes Maria Van Blokland, John Antonius Verhees
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Publication number: 20180030476Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.Type: ApplicationFiled: September 29, 2017Publication date: February 1, 2018Applicant: CellaGenics B.V.Inventors: Arie Pieter OTTE, Michel SIEP, John Antonius VERHEES, Femke HOEKSEMA, Henricus Johannes Maria VAN BLOKLAND
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Patent number: 9790517Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.Type: GrantFiled: June 15, 2011Date of Patent: October 17, 2017Assignee: CellaGenics B.V.Inventors: Arie Pieter Otte, Michel Siep, John Antonius Verhees, Femke Hoeksema, Henricus Johannes Maria Van Blokland
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Patent number: 9650630Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.Type: GrantFiled: August 31, 2011Date of Patent: May 16, 2017Assignee: CellaGenics B.V.Inventors: Arie Pieter Otte, Femke Hoeksema, John Antonius Verhees, Henricus Johannes Maria Van Blokland
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Publication number: 20160160221Abstract: The present invention relates to nucleic acid constructs comprising selectable marker genes in a multicistronic transcription unit for use in the generation and selection of eukaryotic host cells for expression of a gene product of interest. For increased stringency of selection, the coding sequence of the selectable marker may be directed preceded by a relatively short functional open reading frame to reduce the efficiency of translation of the selectable marker, and/or the amino acid sequence of the selectable marker may comprise one or more mutations that reduce the level of resistance provide by the mutated marker as compared to its wild type counterpart. The invention further relates to methods for generating eukaryotic host cells for expression of a gene product of interest, wherein these nucleic acid constructs are used, and to methods for producing a gene product of interest wherein thus generated host cells are applied.Type: ApplicationFiled: February 8, 2016Publication date: June 9, 2016Applicant: CellaGenics B.V.Inventors: Arie Pieter OTTE, Henricus Johannes Maria VAN BLOKLAND, John Antonius VERHEES
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Patent number: 9284563Abstract: The present invention relates to nucleic acid constructs comprising selectable marker genes in a multicistronic transcription unit for use in the generation and selection of eukaryotic host cells for expression of a gene product of interest. For increased stringency of selection, the coding sequence of the selectable marker may be directed preceded by a relatively short functional open reading frame to reduce the efficiency of translation of the selectable marker, and/or the amino acid sequence of the selectable marker may comprise one or more mutations that reduce the level of resistance provide by the mutated marker as compared to its wild type counterpart. The invention further relates to methods for generating eukaryotic host cells for expression of a gene product of interest, wherein these nucleic acid constructs are used, and to methods for producing a gene product of interest wherein thus generated host cells are applied.Type: GrantFiled: June 15, 2010Date of Patent: March 15, 2016Assignee: CELLAGENICS B.V.Inventors: Arie Pieter Otte, Henricus Johannes Maria Van Blokland, John Antonius Verhees
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Publication number: 20120156672Abstract: The present invention relates to nucleic acid constructs comprising selectable marker genes in a multicistronic transcription unit for use in the generation and selection of eukaryotic host cells for expression of a gene product of interest. For increased stringency of selection, the coding sequence of the selectable marker may be directed preceded by a relatively short functional open reading frame to reduce the efficiency of translation of the selectable marker, and/or the amino acid sequence of the selectable marker may comprise one or more mutations that reduce the level of resistance provide by the mutated marker as compared to its wild type counterpart. The invention further relates to methods for generating eukaryotic host cells for expression of a gene product of interest, wherein these nucleic acid constructs are used, and to methods for producing a gene product of interest wherein thus generated host cells are applied.Type: ApplicationFiled: June 15, 2010Publication date: June 21, 2012Inventors: Arie Pieter Otte, Henricus Johannes Maria Van Blokland, John Antonius Verhees