Abstract: A paint brush having an adhesive attachment that includes an engagement of a mass of adhesive within one or more pockets in a lower portion of the handle and an engagement of the mass of adhesive with an internal surface of the ferrule to form a permanent assembly. At least one flow passage fluidly connects with the pocket(s) to facilitate and/or permit flow of the adhesive into the pocket(s) during manufacturing of the paint brush. In addition, the paint brush may include an elongated upper portion having a rigid internal spine that is overlayed and surrounded with a more flexible grip.

Abstract: The invention provides a new method of determining the presence of one or more polymorphic sites in a target nucleic acid sequence. The method comprises determining changes in the melting profile of an amplified product where the amplified product was amplified with a primer pair where one of the primers is labeled, e.g., with a fluorescent label. The method employs a probe that is labeled with a label that interacts with the label on the primer, e.g., a quencher that quenches the fluorescent label on the primer, such that the signal is quenched when the probe and primer are in close proximity. A change in the melting profile of an amplified product in comparison to a control reflects the presence of the polymorphic site.