Patents by Inventor John C. Gerdes
John C. Gerdes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10850276Abstract: Systems and methods that enable the rapid identification of target molecules present in a sample at low concentrations is provided. The system includes a sample volume, and a detection structure that is connected to the sample volume by a conduit. The detection structure includes a microfluidic chip that defines a plurality of fluid channels. The walls of the fluid channels are formed from or covered with a metal oxide to which target molecules attach. After the sample volume has been passed through the detection structure, and in particular through the channels, visible microparticles are passed through the detection structure. The visible microparticles are configured to have an affinity for the target molecules. The microfluidic chip is then imaged to detect visible microparticles, and to thereby obtain information regarding the presence of the target molecules.Type: GrantFiled: February 26, 2018Date of Patent: December 1, 2020Assignee: VISUGEN GLOBAL LLCInventors: John C. Gerdes, Kirsten L. Nelson, Kris Buchanan
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Publication number: 20190078137Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: September 21, 2018Publication date: March 14, 2019Inventors: John C. GERDES, Elaine BEST, Jeffrey M. MARMARO
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Publication number: 20180243738Abstract: Systems and methods that enable the rapid identification of target molecules present in a sample at low concentrations is provided. The system includes a sample volume, and a detection structure that is connected to the sample volume by a conduit. The detection structure includes a microfluidic chip that defines a plurality of fluid channels. The walls of the fluid channels are formed from or covered with a metal oxide to which target molecules attach. After the sample volume has been passed through the detection structure, and in particular through the channels, visible microparticles are passed through the detection structure. The visible microparticles are configured to have an affinity for the target molecules. The microfluidic chip is then imaged to detect visible microparticles, and to thereby obtain information regarding the presence of the target molecules.Type: ApplicationFiled: February 26, 2018Publication date: August 30, 2018Inventors: John C. Gerdes, Kirsten L. Nelson, Kris Buchanan
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Patent number: 9481907Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: June 12, 2014Date of Patent: November 1, 2016Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 9206475Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: January 5, 2015Date of Patent: December 8, 2015Assignee: APPLIED BIOSYSTEMS, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Publication number: 20150125869Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: January 5, 2015Publication date: May 7, 2015Inventors: John C. GERDES, Elaine Best, Jeffrey M. Marmaro
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Publication number: 20140329247Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: June 12, 2014Publication date: November 6, 2014Inventors: John C. GERDES, Elaine Best, Jeffrey M. Marmaro
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Publication number: 20130143754Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: September 14, 2012Publication date: June 6, 2013Applicant: APPLIED BIOSYSTEMS, LLCInventors: John C. GERDES, Elaine Best, Jeffery M. Marmaro
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Patent number: 8304214Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: November 21, 2007Date of Patent: November 6, 2012Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine A. Best, Jeffrey M. Marmaro
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Patent number: 7531328Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: July 7, 2005Date of Patent: May 12, 2009Assignee: Applied Biosystem, LLCInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Publication number: 20090082225Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: ApplicationFiled: April 21, 2008Publication date: March 26, 2009Applicant: APPLERA CORPORATIONInventors: John C. GERDES, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Publication number: 20080131897Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: November 21, 2007Publication date: June 5, 2008Applicant: APPLERA CORPORATIONInventors: John C. GERDES, Elaine Best, Jeffery M. Marmaro
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Patent number: 7361471Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: GrantFiled: May 18, 2006Date of Patent: April 22, 2008Assignee: Applera CorporationInventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Patent number: 7087387Abstract: This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.Type: GrantFiled: October 21, 2003Date of Patent: August 8, 2006Assignee: Applera CorporationInventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Patent number: 7087414Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100–1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: May 19, 2003Date of Patent: August 8, 2006Assignee: Applera CorporationInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Patent number: 6872527Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: GrantFiled: August 31, 2001Date of Patent: March 29, 2005Assignee: XTRANA, Inc.Inventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Publication number: 20040110167Abstract: The invention provides a complete, one-step, fully functional, ready to use lateral flow assay device for the rapid, accurate detection of a target nucleic acid in a fluid sample, wherein the device contains all reagents necessary for the assay in an anhydrous format. The device comprises a sample receiving zone, a labeling zone, and a capture zone. The sample receiving zone may contain one or more oligonucleotides coupled to binding partners and reversibly bound to the capture zone membrane, the labeling zone comprises a visible moiety coupled to a ligand specific for one of the binding partners and reversibly bound to the labeling zone membrane, and the capture zone comprises an capture moiety specific for the second binding partner and immobilized on the capture zone membrane.Type: ApplicationFiled: April 14, 2003Publication date: June 10, 2004Inventors: John C. Gerdes, Roy R. Mondesire, Lara A. Hansen
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Publication number: 20040091925Abstract: This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.Type: ApplicationFiled: October 21, 2003Publication date: May 13, 2004Inventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Publication number: 20030224437Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: May 19, 2003Publication date: December 4, 2003Inventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Patent number: 6649378Abstract: Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequences which may be indicative of genetic defects or contagious diseases, as well as for monitoring efficacy in the treatment of contagious diseases.Type: GrantFiled: November 2, 2000Date of Patent: November 18, 2003Assignee: Xtrana, Inc.Inventors: Diane L. Kozwich, John C. Gerdes