Patents by Inventor JOHN C. JEWETT
JOHN C. JEWETT has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230111712Abstract: Modular Meldrum's acid amine-reactive Michael acceptor (MaMa)—based molecules that are stable probes for high pH environents and bioconjugations. The molecules of the present invention can selectively label and protect lysine residues. In certain embodiments, the molecules can selectively react with lysine at particular pH levels. The probes of the present invention may be used to label proteins in a fluorescent manner, for purification, imaging, or general protein modifications, however the present invention is not limited to the aforementioned applications.Type: ApplicationFiled: March 15, 2021Publication date: April 13, 2023Inventors: John C. Jewett, Garrett Davis, Holly Sofka
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Publication number: 20220274931Abstract: Protected triazabutadiene molecules, such as those according to Formula D or variations thereof, as probes for cellular studies in intact biological systems. The protected triazabutadiene probes selectively release benzene diazonium ions (BDIs) intracellularly, providing a tool for accessing and/or labeling intracellular proteins or molecules prior to cell lysis. The present invention also includes methods for synthesizing the protected triazabutadienes, the protected triazabutadienes themselves, and methods of use.Type: ApplicationFiled: May 23, 2022Publication date: September 1, 2022Inventors: John C. Jewett, Natasha Cornejo, Bismark Amofah, Lindsay E. Guzman, W Mudiyanselage Anjalee Wijetunge, Abigail Shepard, Danielle Johnson
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Patent number: 11339129Abstract: Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with click chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via click chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.Type: GrantFiled: July 31, 2017Date of Patent: May 24, 2022Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONInventors: John C. Jewett, Flora W. Kimani, Lindsay E. Guzman, Brandon M. Cornali
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Publication number: 20210116460Abstract: Methods, systems, and compositions for detecting molecule aggregation, folding, or interactions featuring comparing the amount of labeling of a molecule of interest, such as a protein, in a test sample with an amount of labeling in a control, e.g., a sample wherein the molecule of interest is denatured. If less labeling is present in the test sample as compared to the control sample, the test sample may comprise the molecule of interest in aggregate form, folded form, or interactive form, e.g., interacting with another molecule such as a protein molecule, DNA molecule or RNA molecule. The present invention may be used for detecting or monitoring a disease or condition such as a protein misfolding disease (proteopathy), e.g., amyotrophic lateral sclerosis (ALS), etc.Type: ApplicationFiled: December 22, 2020Publication date: April 22, 2021Inventors: Jacob Schwartz, John C. Jewett, Mahta Moinpour
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Patent number: 10954195Abstract: Triazabutadiene molecules, such as those according to Formula B, which can yield aryl diazonium species, and methods of use of triazabutadiene molecules, for example methods and compositions for yielding an aryl diazonium species from a triazabutadiene molecule, e.g., a protected aryl diazonium species in the form of a triazabutadiene. In some embodiments, an enzyme catalyzes the reaction yielding the aryl diazonium species from the triazabutadiene molecule. As an example, the methods and compositions herein may be used for delivery of drugs.Type: GrantFiled: September 10, 2019Date of Patent: March 23, 2021Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: John C. Jewett, Lindsay E. Guzman, Bereketab T. Mehari, Jie He
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Publication number: 20210002234Abstract: Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with click chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via click chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.Type: ApplicationFiled: July 31, 2017Publication date: January 7, 2021Inventors: John C. Jewett, Flora W. Kimani, Lindsay E. Guzman, Brandon M. Cornali
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Patent number: 10883982Abstract: Methods, systems, and compositions for detecting molecule aggregation, folding, or interactions featuring comparing the amount of labeling of a molecule of interest, such as a protein, in a test sample with an amount of labeling in a control, e.g., a sample wherein the molecule of interest is denatured. If less labeling is present in the test sample as compared to the control sample, the test sample may comprise the molecule of interest in aggregate form, folded form, or interactive form, e.g., interacting with another molecule such as a protein molecule, DNA molecule or RNA molecule. The present invention may be used for detecting or monitoring a disease or condition such as a protein misfolding disease (proteopathy), e.g., amyotrophic lateral sclerosis (ALS), etc.Type: GrantFiled: October 11, 2018Date of Patent: January 5, 2021Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: Jacob Schwartz, John C. Jewett, Mahta Moinpour, Mehrdad Shadmehr
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Publication number: 20200002290Abstract: Triazabutadiene molecules and methods of use of triazabutadiene molecules, for example methods and compositions for yielding an aryl diazonium species from a triazabutadiene molecule, e.g., a protected aryl diazonium species in the form of a triazabutadiene. In some embodiments, an enzyme catalyzes the reaction yielding the aryl diazonium species from the triazabutadiene molecule. As an example, the methods and compositions herein may be used for delivery of drugs.Type: ApplicationFiled: September 10, 2019Publication date: January 2, 2020Inventors: John C. Jewett, Lindsay E. Guzman, Bereketab T. Mehari, Jie He
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Patent number: 10472330Abstract: Triazabutadiene molecules for use in applications. e.g., those involving fluorogenic molecules, e.g., triazabutadiene molecules configured to generate a fluorescent compound when combined with a second molecule, e.g., upon reaction with a tyrosine molecule or other appropriate molecule; and protected triazabutadiene molecules that are stable in acidic conditions and are adapted to release an active (e.g., acid-labile} triazabutadiene molecule upon appropriate conditions, and methods for producing and using said protected or releasable triazabutadiene molecules (and products of cleavage).Type: GrantFiled: August 11, 2016Date of Patent: November 12, 2019Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: John C. Jewett, Lindsay E. Guzman, Flora W. Kimani, Bereketab T. Mehari, Mehrdad Shadmehr
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Publication number: 20190041385Abstract: Methods, systems, and compositions for detecting molecule aggregation, folding, or interactions featuring comparing the amount of labeling of a molecule of interest, such as a protein, in a test sample with an amount of labeling in a control, e.g., a sample wherein the molecule of interest is denatured. If less labeling is present in the test sample as compared to the control sample, the test sample may comprise the molecule of interest in aggregate form, folded form, or interactive form, e.g., interacting with another molecule such as a protein molecule, DNA molecule or RNA molecule. The present invention may be used for detecting or monitoring a disease or condition such as a protein misfolding disease (proteopathy), e.g., amyotrophic lateral sclerosis (ALS), etc.Type: ApplicationFiled: October 11, 2018Publication date: February 7, 2019Inventors: Jacob Schwartz, John C. Jewett, Mahta Moinpour, Mehrdad Shadmehr
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Patent number: 10125105Abstract: Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with click chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via click chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.Type: GrantFiled: February 8, 2017Date of Patent: November 13, 2018Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: John C. Jewett, Flora W. Kimani, Lindsay Guzman, Brandon M. Cornali
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Publication number: 20180230106Abstract: Triazabutadiene molecules for use in applications. e.g., those involving fluorogenic molecules, e.g., triazabutadiene molecules configured to generate a fluorescent compound when combined with a second molecule, e.g., upon reaction with a tyrosine molecule or other appropriate molecule; and protected triazabutadiene molecules that are stable in acidic conditions and are adapted to release an active (e.g., acid-labile} triazabutadiene molecule upon appropriate conditions, and methods for producing and using said protected or releasable triazabutadiene molecules (and products of cleavage).Type: ApplicationFiled: August 11, 2016Publication date: August 16, 2018Inventors: John C. Jewett, Lindsay E. Guzman, Flora W. Kimani, Bereketab T. Mehari, Mehrdad Shadmehr
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Patent number: 10047061Abstract: Water-soluble triazabutadiene molecules and methods for producing and using such compounds. The triazabutadiene molecules may be more labile at pH levels below physiological pH, such as pH 7, pH 6, pH 5, etc. The triazabutadiene molecules and compounds may be used for depositing diazonium salt and/or cargo in a pH-sensitive manner. The triazabutadiene molecules may alternatively be cleaved in reducing conditions or as a light-catalyzed reaction. The compounds herein may be used for delivery of drugs, as part of detection systems, or for other applications such as underwater adhesive applications.Type: GrantFiled: June 10, 2015Date of Patent: August 14, 2018Assignee: Arizona Board of Regents on Behalf of The University of ArizonaInventors: John C. Jewett, Flora W. Kimani
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Patent number: 10016498Abstract: The present disclosure provides modified bacteria and modified peptidoglycan comprising modified D-amino acids; compositions comprising the modified bacteria or peptidoglycan; and methods of using the modified bacteria or peptidoglycan. The modified D-amino acids include a bioorthogonal functional group such as an azide, an alkyne or a norbornene group. Also provided are modified peptidoglycans conjugated to a molecule of interest via a linker.Type: GrantFiled: September 14, 2017Date of Patent: July 10, 2018Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Mary Sloan Siegrist Palmore, John C. Jewett, Chelsea G. Gordon, Peyton Shieh
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Publication number: 20180000929Abstract: The present disclosure provides modified bacteria and modified peptidoglycan comprising modified D-amino acids; compositions comprising the modified bacteria or peptidoglycan; and methods of using the modified bacteria or peptidoglycan. The modified D-amino acids include a bioorthogonal functional group such as an azide, an alkyne or a norbornene group. Also provided are modified peptidoglycans conjugated to a molecule of interest via a linker.Type: ApplicationFiled: September 14, 2017Publication date: January 4, 2018Inventors: Carolyn R. Bertozzi, Mary Sloan Siegrist Palmore, John C. Jewett, Chelsea G. Gordon, Peyton Shieh
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Publication number: 20170320834Abstract: Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with dick chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via dick chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.Type: ApplicationFiled: February 8, 2017Publication date: November 9, 2017Inventors: John C. Jewett, Flora W. Kimani, Lindsay Guzman, Brandon M. Cornali
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Patent number: 9789180Abstract: The present disclosure provides modified bacteria and modified peptidoglycan comprising modified D-amino acids; compositions comprising the modified bacteria or peptidoglycan; and methods of using the modified bacteria or peptidoglycan. The modified D-amino acids include a bioorthogonal functional group such as an azide, an alkyne or a norbornene group. Also provided are modified peptidoglycans conjugated to a molecule of interest via a linker.Type: GrantFiled: March 31, 2016Date of Patent: October 17, 2017Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Mary Sloan Siegrist Palmore, John C. Jewett, Chelsea G. Gordon, Peyton Shieh
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Publication number: 20170114033Abstract: Water-soluble triazabutadiene molecules and methods for producing and using such compounds. The triazabutadiene molecules may be more labile at pH levels below physiological pH, such as pH 7, pH 6, pH 5, etc. The triazabutadiene molecules and compounds may be used for depositing diazonium salt and/or cargo in a pH-sensitive manner. The triazabutadiene molecules may alternatively be cleaved in reducing conditions or as a light-catalyzed reaction. The compounds herein may be used for delivery of drugs, as part of detection systems, or for other applications such as underwater adhesive applications.Type: ApplicationFiled: June 10, 2015Publication date: April 27, 2017Inventors: John C. Jewett, Flora W. Kimani
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Patent number: 9593080Abstract: Triazabutadiene molecules as cleavable cross-linkers adapted to cross-link components with click chemistry, e.g., clickable triazabutadienes. For example, in some embodiments, the triazabutadienes feature alkyne handles attached to the imidazole portion or the aryl portion of the triazabutadienes, wherein the alkyne handles can link to azide handles (e.g., azide handles disposed on other components) via click chemistry. Also described are methods of producing said clickable triazabutadienes and methods of use of said clickable triazabutadienes. The present invention also features methods of cleaving said clickable triazabutadienes, e.g., for liberating the diazonium species for further chemical reactions.Type: GrantFiled: July 29, 2016Date of Patent: March 14, 2017Assignee: THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONAInventors: John C. Jewett, Flora W. Kimani, Lindsay Guzman, Brandon M. Cornali
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Publication number: 20170021013Abstract: The present disclosure provides modified bacteria and modified peptidoglycan comprising modified D-amino acids; compositions comprising the modified bacteria or peptidoglycan; and methods of using the modified bacteria or peptidoglycan. The modified D-amino acids include a bioorthogonal functional group such as an azide, an alkyne or a norbornene group. Also provided are modified peptidoglycans conjugated to a molecule of interest via a linker.Type: ApplicationFiled: March 31, 2016Publication date: January 26, 2017Inventors: Carolyn R. Bertozzi, Mary Sloan Siegrist Palmore, John C. Jewett, Chelsea G. Gordon, Peyton Shieh