Abstract: The present disclosure describes a method of manufacturing a one-component coating composition. The method can include combining a water-containing additive with a non-reactive resin to form a grinding dispersion. The method can also include grinding the grinding dispersion to a Hegman value of at least 4 to form a ground dispersion. A drying agent can then be added to form a dried slurry. A silane-modified polyurea can be added to the dried slurry to form a one-component coating composition.

Abstract: Disclosed are human pluripotent hematopoietic stem cell (PHSC) enriched compositions and methods for obtaining and using the compositions. A substantially homogeneous population of human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.-, and c-kit receptor positive (KR.sup.+) and capable of in vitro self-renewal and differentiation to members of at least the erythroid, myeloid, and megakaryocytic lineages, is obtained by separating the population from a cellular mixture, for example by cytometric cell sorting techniques. Also disclosed is a method of obtaining persistent maintenance of grafted human hematopoietic cells in a mammal, which includes grafting a mammal in utero with a cellular composition enriched in human hematopoietic cells characterized as CD34.sup.+, HLA-DR.sup.- and capable of in vitro self-renewal and differentiation to members of the lymphoid, myeloid, erythroid and megakaryocytic lineages.