Abstract: The present disclosure relates to methods of detecting nucleic acid using mass spectrometry. The present disclosure further relates to methods of detecting nucleic acids of and/or diagnosing diseases such as HIV and COVID-19 by detecting presence of target nucleic acid molecule in samples. The present disclosure also relates to kits for detecting nucleic acid and for detecting nucleic acids of and/or diagnosing diseases such as HIV and COVID-19.

Abstract: A fairing for a power generation machine, the fairing comprising: a first layer comprising a metallic material; a second layer comprising a composite; and a third layer positioned between the first layer and the second layer, the third layer being configured to attenuate acoustic waves over a predetermined frequency range.

Abstract: Disclosed are effective methods for activating, washing, specifically extracting and purifying cannabinoids from Cannabis plant tissues using heat activation, washing impurities away with a polar solvent, optionally modified with an organic acid, base, surfactant or inorganic salt, extracting the activated non-polar cannabinoids with a potable selective solvent such as ethanol. The extracted active ingredients may be purified by chromatography and detected and quantified by mass spectrometry with external or isotopic or otherwise labelled standards.

Abstract: A combustion chamber tile comprising a main body having a first surface and a second surface. The tile having a peripheral wall extending around the edges of the tile and projecting from the first surface. The main body and the peripheral wall of the tile comprising a monolithic structure consisting of consolidated powder material. The peripheral wall of the tile having at least one region consisting of partially consolidated powder material. The at least one region provides a region with reduced stiffness in the peripheral wall enabling the tile to better conform to the shape of an annular wall.

Abstract: A fairing for a power generation machine, the fairing comprising: a first layer comprising a metallic material; a second layer comprising a composite; and a third layer positioned between the first layer and the second layer, the third layer being configured to attenuate acoustic waves over a predetermined frequency range.

Abstract: Disclosed are effective methods for activating, washing, specifically extracting and purifying cannabinoids from Cannabis plant tissues using heat activation, washing impurities away with a polar solvent, optionally modified with an organic acid, base, surfactant or inorganic salt, extracting the activated non-polar cannabinoids with a potable selective solvent such as ethanol. The extracted active ingredients may be purified by chromatography and detected and quantified by mass spectrometry with external or isotopic or otherwise labelled standards.

Abstract: Disclosed are sensitive methods for detecting a target substance in low concentrations. The method can include immobilizing a target substance to a solid phase; incubating the immobilized target substance with a reporter enzyme detection probe in solution under conditions for forming target: enzyme detection probe complexes; washing the solid phase to remove any unbound reporter enzyme detection probe; incubating the target: enzyme detection probe complex with a reporter enzyme detection probe substrate in substrate reaction solution to generate one or more ionizable products; and detecting one or more of the one or more ionizable products using mass spectrometry (MS).

Abstract: Disclosed are sensitive methods for detecting a target substance in low concentrations. The method can include immobilizing a target substance to a solid phase; incubating the immobilized target substance with a reporter enzyme detection probe in solution under conditions for forming target: enzyme detection probe complexes; washing the solid phase to remove any unbound reporter enzyme detection probe; incubating the target: enzyme detection probe complex with a reporter enzyme detection probe substrate in substrate reaction solution to generate one or more ionizable products; and detecting one or more of the one or more ionizable products using mass spectrometry (MS).

Abstract: A method for capturing activated receptor signaling complexes from live cells, utilizing bead based biology wherein live cells are contacted with ligand coated beads to form bead binding sites and thereby initiating formation of a ligand-receptor complex at said bead binding site; and a process for distinguishing and confirming non-specifically bound proteins from specifically bound receptor complexes by utilization of one or more methods of biochemical or biophysical analysis, thereby providing, in a preferred embodiment, a utilization of confocal microscopy and proteomic mass spectroscopy.

Abstract: Reforestation of cut forest regions is effected by broadcasting comminuted slash or cone-bearing roadside logging residue from delimbing of freshly-cut trees over the region from which the trees are cut. By comminuting the slash live seeds are released from cones contained in the slash and are returned to the forested area. In this way, an inexpensive reforestation procedure is provided and biodiversity is retained.

Abstract: A method for attaching a polymethylmethacrylate haptic having a colored core to an optic of an intraocular lens, containing the steps of providing the periphery of the optic with a pair of intersecting holes, inserting an end of the haptic into one hole, inserting an end of a polymethylmethacrylate anchor strand having a colored core into the second hole so that the end of the anchor strand and the end of the haptic intersect, aiming a laser capable of a transmitting energy of a visible wavelength at the intersection of the first and second holes, firing the laser through the optic at the point of intersection of the end of the haptic and the end of the anchor strand, wherein the optic transmits, but essentially does not absorb, the laser energy and the core the anchor strand and the core of the haptic absorb a portion of the laser energy sufficient to fuse the end of the anchor strand and the end of the haptic at their point of intersection.