Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.

Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.

Abstract: The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.

Abstract: The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.

Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.

Abstract: An apparatus for and a method of forming a metal coated with a polymer is achieved through the provision of a reservoir containing a cooling liquid having a relative undisturbed upper surface. A metal coated with a polymer is introduced into the cooling liquid through the upper surface and is subject to quenching liquid at a temperature T.sub.q directed at the polymer coated metal substantially immediately below the cooling liquid upper surface and substantially along the entire predetermined width thereof. This achieves rapid and uniform cooling in the absence of bubble formation and/or quenching liquid surface agitation to assure desired crystalline formation of the polymer absent the polymer blemishes.

Abstract: A data buffer includes parallel and serial data ports connected to one or more equipment modules that produce output data or respond to input commands. The data buffer also includes a serial control port communicating with a host computer. The data buffer receives and stores output data from the equipment modules via the data ports. The buffer configures each data port to match the data transfer protocol (baud rate, parity checking etc.) of the equipment module to which it is connected in response to input commands transmitted from the host computer via the control port. The host computer may also command the buffer to immediately route incoming data on one data port outward on one or more other selected data or control ports or to store incoming data for later transmission via a selected port in response to an input command. The host computer may additionally command the buffer to periodically transmit a stored data string outward on one or more designated data ports.

Abstract: A subscriber loop tester for testing local loops of telephone switching networks has a partitioned "U" interface and an internal bus architecture for converting various transceiver circuit output data formats to a common data format. The "U" interface has a high impedance transformer meeting bandwidth and line matching requirements for different line codes and protocols for ISDN telecommunication systems. The transformer is selectively coupled to option cards each containing circuitry having AC and DC terminations matching specific line codes and protocols for ISDN telecommunications systems and other types of telecommunications systems. The option cards further contain programmable attenuators for producing a proper nominal signal level as a function of the specific line code and protocol and providing variable insertion loss. A high speed bus is selectively coupled to the option cards for coupling the formatted transceiver circuit output data between the cards and a digital bus translator.

Abstract: A refractory tile for a suspended wall of a glass furnace includes upper and lower horizontal faces having respective raised and depressed tongue and groove portions. The tile is adapted to be laid in overlapping or bonded courses such that the tongue and groove portions of a tile overlap and matingly engage the tongue and groove portions of two tiles in a subjacent course of tiles. Each tile also includes vertical side edges having mating ridges and grooves. Each of the tiles further includes a neck portion outwardly extending from a cold face edge thereof. The neck portion defines open spaces between adjacent tiles for insertion of insulation batts therein. The horizontal faces of the tiles also have slots formed therein for engagement with one of the hanger members. The slots have one of a transverse T-portion or a recessed portion formed therein and spaced inwardly from the cold face of the tile which are adapted to engage the hangers.

Abstract: A blanket charger for feeding raw batch material into a glass making furnace, particularly an end fired, sidewall fed type furnace. The charger has a main support frame with a raw batch hopper chute thereon and a reciprocable charger plate positioned under the hopper chute for receiving raw batch material therefrom. The charger plate is reciprocally moved by a fluid driven cylinder and piston device motivated by a fluid, preferably a nonflammable, water-glycol solution or by air. The travel distance of the charger plate in the forward and rearward strokes is selectively controlled by a pair of adjustable striker rod assemblies positioned on the charger plate which alternately contact a pair of frame mounted limit switches. Stroke speed of the cylinder may be varied by selective adjustment of appropriate control devices.

Abstract: The present invention is directed to monoclonal antibodies, and hybridomas which produce them, which are reactive with a highly conserved epitope present on potyviruses and insignificantly reactive with other plant viruses, as well methods of using these monoclonal antibodies to detect potyviruses.

Abstract: A suspended back wall for a high temperature furnace including a plurality of spaced-apart, vertically extending columns having upper portions supported between spaced girder members to bear loading while permitting lateral thermal expansive and contractive movement of the upper portions of the columns. Lower end portions of the columns are attached to a main support beam which horizontally traverses the width of the suspended wall. A plurality of interlocking refractory tiles are secured on the vertical columns by cast metal hangers. A plurality of insulation slabs or batts are inserted in specially formed pockets between adjacent refractory tiles and columns at a rear face thereof. An air chamber is formed along the rear of the vertical columns and an air duct is provided along the bottom thereof to supply a regulated forced air flow to the air chamber.

Abstract: Apparatus for detecting acoustic disturbances include an electrical circuit for processing electrical signals generated by an acoustic sensor in response to acoustic disturbances. The processing circuitry converts the electrical data signals into output signals wherein the information about the input audio signals is carried by the frequencies of the output signals. High frequency signals may be isolated and processed to provide relatively low frequency digital pulse signals which may be readily transmitted over wirelines in a logging operation. The sensor apparatus may include a stack of piezoelectric crystals which are electrically connected by means of pressure contacts.

Abstract: A constant carrier watchdog circuit for an r.f. modem detects an anomalous constant "carrier on" condition in the modem. In response to this anomalous condition a signal is generated and output to the modem. The signal causes the carrier frequency to shift out of the data channel frequency bands so that the anomalous modem does not affect other users on a telecommunication network.

Abstract: A process for the concentration of pigment press cakes including feeding the press cakes to rollers and simultaneously applying a vacuum.