Abstract: An array of sliding sleeve valves are uniquely addressable without control lines or wires to open for a treatment and then close and then selectively open for production. The discrete movements employ an available pressure source such as tubing pressure and change the piston areas on opposed sides of a sliding sleeve valve to get the desired movements. Access valves to tubing pressure can be actuated in a desired sequence with signals such as acoustic or electromagnetic, for example. Access to one piston area that communicates opposed and offsetting piston areas to the tubing hydrostatic can be achieved with a straddle tool breaking a rupture disc. The piston is then in pressure balanced and can be moved in a desired direction with the straddle tool straddling access locations to the piston from above or below.

Abstract: A zone to be treated comprises a plurality of sliding sleeve valves. The sleeve defined opposed chambers charged with pressurized fluid on opposed sides of the sleeve. Valves responsive to a remote signal with no borehole intervention change the pressure balance on the sleeve to get it to open from a closed position and then close and then to reopen for production. One way this is done is by sequential pressure bleeding off from the opposed chambers. A zone having multiple such valves can be treated without need for dropping balls and subsequent milling out, which allows production to commence sooner with reduced restrictions to flow from the ball seats and without the debris associated from a milling operation.

Abstract: A sealing element is flanked by wedge-shaped extrusion ring assemblies. The rings climb a ramp on an adjacent pedestal ring on the way out to the borehole wall. Depending on the dimension of the gap to be spanned the extrusion rings slide part way up the pedestal ring ramp or to the top of the pedestal ring. An anchor ring is initially forced up an opposite ramp of the pedestal ring. If the sealing gap is short the anchor ring touches the borehole wall to act as an anchor for the plug while remaining spaced apart from the extrusion ring assembly. For larger gaps the anchor ring moves out far enough to the borehole wall and in contact with the extrusion ring located on top of the pedestal so that reaction forces are directed to keep the anchor ring wedged in position for support of the extrusion ring assembly.

Abstract: A method for installing a tool in a downhole system including running the tool to a target location within a tubular structure, creating a magnetic pulse, and urging the tool toward the tubular structure at a minimum velocity of 200 meters per second with the magnetic pulse. A method for actuating a downhole tool including creating a magnetic pulse, coupling the magnetic pulse to a workpiece and moving the workpiece with the pulse, the workpiece achieving a minimum velocity of 200 meters per second.

Abstract: A unique backup ring against ends of a sealing element features axial slots extending part way along a cylindrical segment of the backup ring. The slots end in rounded openings to relieve stress and a part of the cylindrical shape of the backup ring is solid. The slotted end of the cylindrical portion is tapered in section toward the end overlapping the sealing element. The face of the backup ring away from the sealing element is tapered and rides on an adjacent tapered surface away from the mandrel during the setting. The tapered seal end of the backup ring bends to reach the surrounding tubular before the balance of the cylindrical portion reaches the surrounding tubular. Extrusion along the mandrel is stopped by a mandrel seal on an adjacent wedge ring. The mandrel end of the backup ring has a peripheral stiffener to lend rigidity.

Abstract: In one embodiment, a filler ring for use with a packer element with a packer element radial stiffness and a packer element confined circumferential stiffness is disclosed, including, a filler ring body with a filler ring body radial stiffness greater than the packer element radial stiffness and a filler ring body circumferential stiffness less than the packer element confined circumferential stiffness. In another embodiment, a method to selectively deform a packer element with a packer element radial stiffness and a packer element confined circumferential stiffness is disclosed, including providing a filler ring body with a filler ring body radial stiffness greater than the packer element radial stiffness and a filler ring body circumferential stiffness less than the packer element confined circumferential stiffness, deforming the packer in a radial direction via the filler ring body, deforming the filler ring body in an axial direction in response to deformation of the packer element.

Abstract: A sealing element is flanked by wedge-shaped extrusion ring assemblies. The rings climb a ramp on an adjacent pedestal ring on the way out to the borehole wall. Depending on the dimension of the gap to be spanned the extrusion rings slide part way up the pedestal ring ramp or to the top of the pedestal ring. An anchor ring is initially forced up an opposite ramp of the pedestal ring. If the sealing gap is short the anchor ring touches the borehole wall to act as an anchor for the plug while remaining spaced apart from the extrusion ring assembly. For larger gaps the anchor ring moves out far enough to the borehole wall and in contact with the extrusion ring located on top of the pedestal so that reaction forces are directed to keep the anchor ring wedged in position for support of the extrusion ring assembly.

Abstract: The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.

Abstract: In producing methane a bottom hole assembly the borehole may enlarge due to shifting sands in an unconsolidated formation as the methane is produced. The enlargement of the borehole can be sensed in real time such as by using a fiber optic cable. In response to such information parts of the bottom hole assembly near the washout can be isolated or the bottom hole assembly in the vicinity of the washout can be fortified with inserts from the surface to minimize damage from erosion caused by higher velocities resulting from borehole washouts.

Abstract: The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.

Abstract: The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.

Abstract: The present invention provides methods and compositions for the integration of a nucleotide sequence of interest into the genome. Specifically, the methods of the present invention comprise contacting a lentiviral vector with the plasma membrane of an oocyte, early stage embryo, or blastula under conditions that permit the entry of the viral vector and the subsequent integration of the nucleotide sequence of interest into the genome. The methods further comprise culturing the oocyte or embryo contacted with the lentiviral vector under conditions that allow for the formation of a pre-implantation embryo. Subsequently, the pre-implantation embryo can be transferred into a recipient vertebrate where it is allowed to develop into at least one genetically modified animal. The methods and compositions of the invention thereby allow for the production of genetically modified animals, particularly, vertebrates, and particularly mammals.