Patents by Inventor John Oakey
John Oakey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230381730Abstract: Described are methods and devices for the generation of hydrogel particles with micrometer and submicrometer dimensions using oxygen-inhibited partial polymerization, and the particles generated therefrom. The described methods generate particles with dimensions independent of the starting polymerizable solution dimension, for example, a microdroplet. Further, microfluidic flow parameters (e.g. viscosity, flow rate) and photopolymerization process parameters (e.g. optical exposure intensity and duration) are controlled to generate particles with tunable crosslinking density-determined properties including elasticity, diffusivity, and biomolecular display for diverse applications such as drug delivery, tissue engineering cell scaffolds, and single- and multiple-cell therapeutics. Similarly, gradients of crosslinking density-determined properties can be created within single particles through the selection of optical exposure intensity and duration.Type: ApplicationFiled: March 15, 2023Publication date: November 30, 2023Applicant: University of WyomingInventors: John OAKEY, Katie Dongmei LI-OAKEY, Daniel DEBROY, Carl FRICK, Rajib SHAHA
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Publication number: 20230158077Abstract: Embodiments of the present disclosure generally relate to methods and compositions for controlling cellular expression. More specifically, embodiments described herein relate to hydrogel-encapsulated/dispersed cells, methods of forming hydrogel-encapsulated/dispersed cells, and methods of using hydrogel-encapsulated/dispersed cells for controlling production of, for example, secretomes. In an embodiment, a composition for controlling production of secretomes is provided. The composition includes, a hydrogel comprising, in polymerized form, one or more photoreactive monomers and a thiol linker, wherein at least one of the one or more photoreactive monomers comprises a methylene functional group; and one or more cells dispersed or encapsulated within the hydrogel.Type: ApplicationFiled: November 18, 2022Publication date: May 25, 2023Inventors: Benjamin NOREN, John OAKEY, Zhongliang JIANG, Kun JIANG
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Patent number: 11642642Abstract: Described are methods and devices for the generation of hydrogel particles with micrometer and submicrometer dimensions using oxygen-inhibited partial polymerization, and the particles generated therefrom. The described methods generate particles with dimensions independent of the starting polymerizable solution dimension, for example, a microdroplet. Further, microfluidic flow parameters (e.g. viscosity, flow rate) and photopolymerization process parameters (e.g. optical exposure intensity and duration) are controlled to generate particles with tunable crosslinking density-determined properties including elasticity, diffusivity, and biomolecular display for diverse applications such as drug delivery, tissue engineering cell scaffolds, and single- and multiple- cell therapeutics. Similarly, gradients of crosslinking density-determined properties can be created within single particles through the selection of optical exposure intensity and duration.Type: GrantFiled: October 17, 2018Date of Patent: May 9, 2023Assignee: UNIVERSITY OF WYOMINGInventors: John Oakey, Katie Dongmei Li-Oakey, Daniel Debroy, Carl Frick, Rajib Shaha
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Publication number: 20220090000Abstract: Embodiments of the present disclosure generally relate to apparatus for isolating biological material, processes for fabricating such apparatus, and processes for using such apparatus. In an embodiment, an apparatus for isolating a biological material is provided. The apparatus includes a fluidic channel disposed over a portion of a substrate. The apparatus further includes a hydrogel structure disposed in the fluidic channel, the hydrogel structure comprising a plurality of wells, wherein each well of the plurality of wells has a diameter from about 1 ?m to about 500 ?m, the hydrogel structure comprising, in polymerized form, one or more photoreactive monomers.Type: ApplicationFiled: September 24, 2021Publication date: March 24, 2022Inventors: John OAKEY, John KISIDAY, Benjamin NOREN
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Publication number: 20220089821Abstract: Embodiments of the present disclosure generally relate to compositions comprising hydrogel-encapsulated/dispersed beta cells, compositions comprising hydrogel-encapsulated/dispersed beta-cell spheroids, processes for forming such compositions, and uses of the compositions. In an embodiment, a composition is provided that includes a first component comprising a hydrogel, the hydrogel comprising, in polymerized form, one or more photoreactive monomers and a thiol linker. The composition further comprises a second component comprising a plurality of beta cells dispersed or encapsulated within the hydrogel.Type: ApplicationFiled: September 22, 2021Publication date: March 24, 2022Inventors: John OAKEY, Benjamin NOREN, Zhongliang JIANG, Kun JIANG
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Publication number: 20220064624Abstract: Embodiments of the present disclosure generally relate to compositions that include hydrogel-encapsulated/dispersed cells, compositions including hydrogel-encapsulated/dispersed cells, and to processes for forming such hydrogel-encapsulated/dispersed cells and compositions thereof. The compositions can be used for, e.g., therapeutic applications. In some examples, the hydrogel-encapsulated/dispersed cells are formed using photoreactive groups chemically attached to polyethylene glycol to form a material which, upon exposure to a desired wavelength or wavelength range of light, reacts to form a cross-linked hydrogel network.Type: ApplicationFiled: August 31, 2021Publication date: March 3, 2022Inventors: Benjamin NOREN, John OAKEY, Zhongliang JIANG, Kun JIANG
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Patent number: 11262361Abstract: Disclosed herein are photodegradable hydrogels and associated kits for selectively capturing and releasing cells. The hydrogels result from cross linking in the presence of a photoinitiator (1) a macromer having a polymeric backbone structure, a photo labile moiety, and a first linking moiety, and (2) a cell-binding moiety having a second linking moiety. These two components are cross-linked by a polymerization reaction of the linking moieties to form a photodegradable hydrogel incorporating the cell-binding moiety within the hydrogel. Also disclosed are methods of making the hydrogels, and methods of using the hydrogels for selectively capturing and releasing cells and for detecting cells in a fluid. Such methods can be used to detect the presence and quantity of certain rare cell types in a biological fluid.Type: GrantFiled: July 18, 2014Date of Patent: March 1, 2022Assignee: The General Hospital CorporationInventors: Mark W. Tibbitt, Kristi S. Anseth, April M. Kloxin, Mehmet Toner, John Oakey, Ajay Shah
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Publication number: 20210171587Abstract: The present disclosure generally relates to compositions comprising fibrin and to methods of forming such compositions. In an embodiment, a method of forming fibrin particles is provided. The method includes introducing a buffer, a fibrinogen solution, and a thrombin solution to a first end of a microfluidic device to form a mixture, the buffer comprising one or more amino acids. The method further includes contacting the mixture with a fluorocarbon oil and a surfactant to form fibrinogen-containing particles, and applying positive pressure to the microfluidic device to cause the fibrinogen-containing particles to flow towards a second end of the microfluidic device. The method further includes collecting the fibrinogen-containing particles at the second end of the microfluidic device; and polymerizing the fibrinogen-containing particles to form fibrin particles.Type: ApplicationFiled: December 4, 2020Publication date: June 10, 2021Inventors: John OAKEY, Alan STENQUIST
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Publication number: 20210025881Abstract: Embodiments of the present disclosure generally relate to apparatus and methods for analyte detection. More specifically, embodiments of the present disclosure relate to a microscale biosensing platform based upon the rehydration-mediated swelling of functionalized hydrogel structures and rapid capture of target analyte(s) by induced convective flow. In an embodiment is provided an apparatus for analyte detection that includes a fluidic channel coupled to a substrate and a hydrogel structure coupled to the fluidic channel. The hydrogel structure includes one or more surface-functionalized hydrogel features, wherein at least one of the one or more surface-functionalized hydrogel features comprises a chemically-bound probe, the chemically-bound probe to bind an analyte, and wherein the hydrogel structure is at least partially dehydrated. Apparatus described herein can also include a detector. Methods of detecting analytes are also described herein.Type: ApplicationFiled: July 22, 2020Publication date: January 28, 2021Inventors: John OAKEY, Mark H. HARPSTER, Cheng CHENG
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Patent number: 10828642Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: GrantFiled: July 30, 2019Date of Patent: November 10, 2020Assignee: ABBOTT POINT OF CARE INC.Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell
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Publication number: 20200254417Abstract: Described are methods and devices for the generation of hydrogel particles with micrometer and submicrometer dimensions using oxygen-inhibited partial polymerization, and the particles generated therefrom. The described methods generate particles with dimensions independent of the starting polymerizable solution dimension, for example, a microdroplet. Further, microfluidic flow parameters (e.g. viscosity, flow rate) and photopolymerization process parameters (e.g. optical exposure intensity and duration) are controlled to generate particles with tunable crosslinking density-determined properties including elasticity, diffusivity, and biomolecular display for diverse applications such as drug delivery, tissue engineering cell scaffolds, and single- and multiple-cell therapeutics. Similarly, gradients of crosslinking density-determined properties can be created within single particles through the selection of optical exposure intensity and duration.Type: ApplicationFiled: October 17, 2018Publication date: August 13, 2020Applicant: University of WyomingInventors: John OAKEY, Katie Dongmei LI-OAKEY, Daniel DEBROY, Carl FRICK, Rajib SHAHA
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Publication number: 20190351406Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: ApplicationFiled: July 30, 2019Publication date: November 21, 2019Applicant: Abbott Point of Care Inc.Inventors: Kevin John DOYLE, Paul WILKINS, Mick WITHERS, Adrian COOPER, John Oakey NOELL
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Patent number: 10406523Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: GrantFiled: January 30, 2017Date of Patent: September 10, 2019Assignee: Abbott Point of Care Inc.Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell
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Publication number: 20170145169Abstract: Provided herein are methods utilizing microfluidics for the oxygen-controlled generation of microparticles and hydrogels having controlled microparticle sizes and size distributions and products from provided methods. The included methods provide the generation of microparticles by polymerizing an aqueous solution dispersed in a non-aqueous continuous phase in an oxygen-controlled environment. The process allows for control of size of the size of the aqueous droplets and, thus, control of the size of the generated microparticles which may be used in biological applications.Type: ApplicationFiled: October 26, 2016Publication date: May 25, 2017Inventors: John OAKEY, Kaspars KRUTKRAMELIS, Bingzhao XIA
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Publication number: 20170136455Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: ApplicationFiled: January 30, 2017Publication date: May 18, 2017Inventors: Kevin John DOYLE, Paul WILKINS, Mick WITHERS, Adrian COOPER, John Oakey NOELL
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Patent number: 9592507Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing including a top portion having a first substantially rigid zone and a substantially flexible zone, a bottom portion separate from the top portion including a second substantially rigid zone, and at least one sensor recess containing a sensor. The top portion and the bottom portion are bonded to form the cartridge having a conduit over at least a portion of the sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: GrantFiled: June 22, 2012Date of Patent: March 14, 2017Assignee: ABBOTT POINT OF CARE INC.Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell
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Patent number: 9415389Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing comprising a first substantially rigid zone, a second substantially flexible zone, a hinge region, and at least one sensor recess containing a sensor. The housing is foldable about said hinge region to form a cartridge having a conduit over at least a portion of said sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: GrantFiled: June 9, 2014Date of Patent: August 16, 2016Assignee: Abbott Point of Care Inc.Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell
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Publication number: 20160153999Abstract: Disclosed herein are photodegradable hydrogels and associated kits for selectively capturing and releasing cells. The hydrogels result from cross linking in the presence of a photoinitiator (1) a macromer having a polymeric backbone structure, a photo labile moiety, and a first linking moiety, and (2) a cell-binding moiety having a second linking moiety. These two components are cross-linked by a polymerization reaction of the linking moieties to form a photodegradable hydrogel incorporating the cell-binding moiety within the hydrogel. Also disclosed are methods of making the hydrogels, and methods of using the hydrogels for selectively capturing and releasing cells and for detecting cells in a fluid. Such methods can be used to detect the presence and quantity of certain rare cell types in a biological fluid.Type: ApplicationFiled: July 18, 2014Publication date: June 2, 2016Inventors: Mark W. Tibbitt, Kristi S. Anseth, April M. Kloxin, Mehmet Toner, John Oakey, Ajay Shah
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Publication number: 20140301915Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing comprising a first substantially rigid zone, a second substantially flexible zone, a hinge region, and at least one sensor recess containing a sensor. The housing is foldable about said hinge region to form a cartridge having a conduit over at least a portion of said sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: ApplicationFiled: June 9, 2014Publication date: October 9, 2014Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell
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Patent number: 8747774Abstract: The invention relates to a cartridge housing for forming a cartridge capable of measuring an analyte or property of a liquid sample. The housing comprising a first substantially rigid zone, a second substantially flexible zone, a hinge region, and at least one sensor recess containing a sensor. The housing is foldable about said hinge region to form a cartridge having a conduit over at least a portion of said sensor. The invention also relates to methods for forming such cartridges and to various features of such cartridges.Type: GrantFiled: December 17, 2010Date of Patent: June 10, 2014Assignee: Abbott Point of Care Inc.Inventors: Kevin John Doyle, Paul Wilkins, Mick Withers, Adrian Cooper, John Oakey Noell