Abstract: Methods and a double vacuum processing system are presented. A method of performing a double vacuum debulk and cure on a composite laminate is presented. A composite laminate is heated to begin a double vacuum debulk of the composite laminate while vacuum is pulled in a first vacuum zone containing the composite laminate and a second vacuum zone containing the first vacuum zone. Pressure within the second vacuum zone is increased to above atmospheric pressure. Heating the composite laminate is continued to complete a cure cycle for the composite laminate while the second vacuum zone is above atmospheric pressure.

Abstract: An apparatus, system, device, and the like, for a liquid nitrogen dispensing head that may be used in a system for cooling aggregate, such as may be found in a batch plant involved in producing concrete. The dispensing head is positioned over a conveyor belt that conveys aggregate to a mixing apparatus to produce concrete. The head dispenses liquid nitrogen onto the aggregate to cool the aggregate and may include interior chambers separated by one or more baffles. The chambers may reduce the pressure of the liquid nitrogen onto the aggregate in relation to the flow pressure of the liquid nitrogen entering the dispensing head. In another example, an assembly may include sidewalls that extend to the aggregate on either side of the head to form a cooling tunnel through which the aggregate is conveyed and is exposed to the liquid nitrogen being dispensed from the head.

Abstract: An air hose includes a corrugated flexible hose. The air hose also includes a first hose end section mechanically coupled to the corrugated flexible hose. The first hose end section includes a pressure sensor communicatively coupled to a warming unit. The first hose end section is configured to releasably couple to a pneumatic convective device. The air hose also includes a second hose end section mechanically coupled to the corrugated flexible hose. The second hose end section is configured to couple to the warming unit.

Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

Abstract: Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

Abstract: A disposable package makes a beverage when liquid is supplied thereto by a beverage forming apparatus. The package comprises an impermeable capsule including a bottom wall and a tubular side wall. The side wall includes an annular shelf extending downwardly and outwardly from an upper end thereof. The side wall includes vertical indentations formed therein wherein upper ends thereof terminate at the annular shelf. An annular upper lip, which surrounds an opening to the interior space, is configured to locate the package in a receptacle of the apparatus. A lid seals the opening. A liquid supply component supplies liquid to the interior space of the capsule which combines with a flavor medium to form a beverage. Pressure build up in the interior space of the capsule deforms the capsule such that the annular shelf tears away from at least one indentation to form one or more through openings. The beverage dispenses through the openings.

Abstract: A handheld electronic device protective case includes a shell with a retention structure attached to the shell. The retention structure is capable or retaining an e-vaping article, where the e-vaping article is a blister pack or an e-vaping item. The retention structure can be a storage portion with a holding member that slideably receives and retains a blister pack, and a locking member that impedes the blister pack from sliding from the storage portion. The retention structure can also include a cavity or a compartment that receives and retains a cylindrically-shaped e-vaping item.

Abstract: Provided is a novel approach for generating oligonucleotide probes and the use of these probes in gene expression profiling, by hybridization to test oligonucleotides on arrays or beads. This approach involves labeling of the complement oligonucleotide probes using a mixture of dye or hapten labeled-ddNTPs in solution. The labeled oligonucleotide probes are then used to hybridize to the test oligonucleotides on the solid support. Success in hybridization is monitored by associated signal on the solid support. This approach greatly reduces hybridization time, due to the simplification of the probe content. It is especially useful when analyzing a small number of genes, such as a signature set of genes for a disease or condition.

Abstract: This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support.

Abstract: Improved processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed. The product of this amplification is used for analysis by restriction enzyme digestion or DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the method are also described. Also described are further uses of this amplified DNA in sequencing, genotyping and haplotyping, and other molecular biology applications.

Abstract: Error-testing of merchant messages to be used with point-of-sale (POS) devices is performed by an automated system that receives a formatted message to be tested from a merchant or an agent of the merchant, performs a computer-implemented evaluation of the message according to predefined message-validation rules to detect one or more errors, and notifies the merchant or the agent of evaluation results. The evaluation results include a listing of each detected error, if any.

Abstract: This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support.

Abstract: The present invention describes terminal phosphate blocked nucleoside polyphosphates that are stable at high temperature and their use in nucleic acid amplification and analysis. Current invention further describes charge modified terminal phosphate blocked nucleoside polyphosphates for improved incorporation and direct loading of nucleic acid sequencing reactions onto separating media.

Abstract: An article of padding which attaches to a ladder to facilitate carrying the ladder by a person. This invention is a channel member which attaches to the rail of the ladder at a point where the ladder would rest on the shoulder. The channel member is of suitable material to snap on to the rail of the ladder and remain in place indefinitely. The channel member may have foam rubber or similar padding material affixed to its surfaces. The channel member may be notched to accommodate exiting hardware on some ladders.

Abstract: The universal dictation input apparatus and method disclosed employs multiple wireless dictation devices (“WDD”) and base stations each of which include a 900 MHz radio for communicating with one another over selected radio frequency (“RF”) channel pairs to conduct dictation sessions on centralized dictation systems (“CDSs”). Each base station is uniquely assigned a single channel pair. The WDDs are programmed with code representing the exclusive channels of a select number of base stations. When a user takes a WDD off-hook, a WDD sequentially and cyclically scans for carrier transmitted by authorized base stations until an idle base station is identified and linked to over the authorized channel. The user of a WDD must be authorized to conduct dictation sessions on one or more CDSs. To this end the WDDs includes a dialing address keypad to address authorized CDSs. The CDSs are third party systems addressed from a WDD by DTMF dialing digits.

Abstract: The invention generally concerns a submersible well pumping system comprising an axially elongated housing having a diameter less than the bore hole of the well, a multi-chamber hydraulically driven diaphragm pump, suspended in the well using coiled tubing, in which the coiled tubing contains one or more electrical cables to provide power to the pump from the surface. The pump is driven by a self-contained, closed loop hydraulic system, activated by an electric or hydraulic motor. The flow of working fluid into and out of the pumping chambers is controlled by a two state snap-acting valve, in turn controlled by a sensor which senses the proximity of the working diaphragm and generates an electrical signal to change the state of the valve, typically when either diaphragm reaches the bottom of the pumping stroke.

Abstract: To prevent entry of contaminants into a craft unit, each of a telephone signal connector (RJ11 jack) and DIN-type digital signalling link connector is replaced with a sealed, multi-pin plug/receptacle type connector which integrates the routing of both telephone communication signals and digital communication signals between the craft unit and an external device. When stored in a storage and battery-charging station, the craft unit is maintained in a communication-ready state, by providing each of the battery-charging holder and the portable craft unit with an additional multi-pin interconnect arrangement, that contains a charging path for the craft unit's internal battery, and a multi-pin signalling path. This multi-pin interconnect arrangement contains both a set of communication signalling pins and a set of terminal pins through which the internal battery of the portable craft unit is charged.

Abstract: A contact-sensitive display screen panel of a craft unit is protected by a pivotable lid. The lid is rotatably supported by lid pivot attachments that enable the lid to be pivoted between closed and open positions, and to be stably locked as a support member in its fully extended attitude. Lid pin elements extend from opposite ends of a lid pin support element and engage left side and right side hinge assemblies. Each lid pin element has a tab element translatable along the underside of the lid. Each lid pin element is biased by a compression spring into engagement with a capturing slot in an interior bore of a post of a hinge arm element of a respective one of hinge assemblies. To rotate the lid from its open position, to its fully extended position, the craftsperson translate the tab elements against the biasing of compression springs, so that the lid pin element becomes unlocked from a slot in the interior bore of the hinge arm element of a respective hinge assembly.

Abstract: An electronic ballast circuit for two fluorescent lamps connected in parallel. A single switching inverter connected through a common coupling circuit provides high frequency alternating current for operation of both lamps independently of each other insuring operation of one lamp should the other cease operation. A dual toroidal transformer in the inverter insures both lamps receive current of the same frequency.