Patents by Inventor John Richard Nelson
John Richard Nelson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11834670Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.Type: GrantFiled: April 19, 2017Date of Patent: December 5, 2023Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLCInventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith
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Patent number: 11726078Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.Type: GrantFiled: November 6, 2020Date of Patent: August 15, 2023Assignees: General Electric Company, Tokitae LLCInventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, David Roger Moore, Paul Michael Smigelski, Jr., John Thomas Connelly, Benjamin David Grant, Bernhard Hans Weigl
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Patent number: 11714084Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.Type: GrantFiled: November 5, 2020Date of Patent: August 1, 2023Assignee: General Electric CompanyInventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, Brian Christopher Bales, David Roger Moore, Paul Michael Smigelski, Jr.
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Patent number: 11555215Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.Type: GrantFiled: January 29, 2019Date of Patent: January 17, 2023Assignee: GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTDInventors: Bing Li, David Roger Moore, William Christopher Alberts, John Richard Nelson
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Publication number: 20220389473Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.Type: ApplicationFiled: July 19, 2022Publication date: December 8, 2022Inventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
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Patent number: 11421259Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.Type: GrantFiled: July 20, 2018Date of Patent: August 23, 2022Assignee: Global Life Sciences Solutions Operations UK LtdInventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
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Publication number: 20220243243Abstract: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.Type: ApplicationFiled: June 9, 2020Publication date: August 4, 2022Inventors: Brian Michael Davis, Erik Leeming Kvam, John Richard Nelson, Lisa A. Lowery, Wei Gao
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Patent number: 11268116Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.Type: GrantFiled: March 30, 2018Date of Patent: March 8, 2022Assignee: GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTDInventors: Robert Scott Duthie, John Richard Nelson, Anuradha Sekher
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Publication number: 20210338805Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.Type: ApplicationFiled: April 29, 2021Publication date: November 4, 2021Inventors: Christopher Michael Puleo, Victoria Eugenia Cotero, John Richard Nelson
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Patent number: 11142758Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.Type: GrantFiled: March 20, 2017Date of Patent: October 12, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Erik Leeming Kvam, John Richard Nelson, Gregory Andrew Grossmann, Ryan Charles Heller, Erin Jean Finehout, Christopher Michael Puleo, William Patrick Waters
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Patent number: 11033859Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: GrantFiled: June 19, 2018Date of Patent: June 15, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nicole Lea Wood, Li Zhu, Craig Patrick Galligan
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Patent number: 11001868Abstract: Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/?L to about 35 ng/?L. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.Type: GrantFiled: August 11, 2017Date of Patent: May 11, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Erik Leeming Kvam, John Richard Nelson, Wei Gao
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Publication number: 20210055297Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.Type: ApplicationFiled: November 5, 2020Publication date: February 25, 2021Inventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, Brian Christopher Bales, David Roger Moore, Paul Michael Smigelski
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Publication number: 20210055282Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.Type: ApplicationFiled: November 6, 2020Publication date: February 25, 2021Inventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, David Roger Moore, Paul Michael Smigelski, John Thomas Connelly, Benjamin David Grant, Bernhard Hans Weigl
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Patent number: 10870845Abstract: A method is provided herein, wherein the method of capturing a target nucleic acid, comprises applying a nucleic acid capture probe to a capture zone of a needs definition, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate. The method further comprises applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate; wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization to the capture zone.Type: GrantFiled: December 11, 2014Date of Patent: December 22, 2020Assignee: Global Life Sciences Solutions Operations UK LtdInventors: John Richard Nelson, Bing Li
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Publication number: 20200392554Abstract: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.Type: ApplicationFiled: June 13, 2019Publication date: December 17, 2020Inventors: Brian Michael Davis, Erik Leeming Kvam, John Richard Nelson, Lisa Anne Lowery, Wei Gao
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Patent number: 10837962Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.Type: GrantFiled: December 20, 2017Date of Patent: November 17, 2020Assignee: GENERAL ELECTRIC COMPANYInventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, Brian Christopher Bales, David Roger Moore, Paul Michael Smigelski, Jr.
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Patent number: 10830760Abstract: A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.Type: GrantFiled: December 20, 2017Date of Patent: November 10, 2020Assignee: GENERAL ELECTRIC COMPANYInventors: Matthew Jeremiah Misner, Gregory Andrew Grossmann, Cathryn Ellen Olsen, John Richard Nelson, David Roger Moore, Paul Michael Smigelski, Jr., John Thomas Connelly, Benjamin David Grant, Bernhard Hans Weigl
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Patent number: 10787695Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.Type: GrantFiled: June 1, 2017Date of Patent: September 29, 2020Assignee: General Electric CompanyInventors: Christopher Michael Puleo, Christine Lynne Surrette, Erik Leeming Kvam, Steven Yuehin Go, Feng Chen, John Richard Nelson, Craig Patrick Galligan, Radislav Alexandrovich Potyrailo, Gregory Andrew Grossmann
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Patent number: 10655167Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.Type: GrantFiled: April 9, 2018Date of Patent: May 19, 2020Assignee: General Electric CompanyInventors: Ryan Charles Heller, Erik Leeming Kvam, John Richard Nelson