Abstract: An instrument advancement device includes an access connector configured to be connected to a vascular access device, a primary lumen in fluid communication with the access connector, an instrument received within the primary lumen and having a retracted position and an extended position, an advancement member configured to move the instrument between the retracted position and the extended position, and a lumen seal received within the primary lumen and connected to the instrument. The lumen seal defines a space between the lumen seal and the primary lumen when the instrument is in the retracted portion. The lumen seal engages the primary lumen when the instrument is in the advanced position.

Abstract: Methods are provided for labeling and for detecting DNA-interacting proteins with an assayable label, comprising combining the DNA-interacting protein with a mfpA pentapeptide repeat family protein that has been purified and that binds to the protein, wherein the mfpA pentapeptide repeat family protein is bound to an assayable label.

Abstract: The present invention generally relates to methods for treating tuberculosis in a subject comprising administering to the subject an antibiotic in conjunction with clavulanic acid or salt thereof. The antibiotic can be carbapenem (e.g., meropenem or imipenem) or cefuroxime. The present invention also relates to related pharmaceutical compositions and methods for manufacturing said pharmaceutical compositions.

Abstract: The present invention is directed to a method of identifying an acetyltransferase substrate in a sample that includes the steps of: (a) contacting the sample with (i) a reagent comprising a thiol-containing compound, a halo-acetyl-CoA or a halo-acetyl-pantetheine, and (ii) an acetyltransferase, under conditions suitable for acetyltransferase enzyme activity, and (b) identifying a substrate that has formed a base-stable covalent bond to the reagent, wherein the reagent is labeled with a label and/or the acetyltransferase is labeled with an affinity tag, and the substrate is the acetyltransferase substrate.

Abstract: Provided are recombinant pentapeptide repeat family proteins comprising at least one mutation of an i?1, i+1, and/or i+2 amino acid residue. Also provided are vectors comprising a nucleic acid sequence encoding the above-described pentapeptide repeat family proteins, protein libraries comprising the above pentapeptide repeat family proteins, and vector libraries comprising the above-described vectors. Additionally provided are methods of identifying a pentapeptide repeat family protein with an assayable phenotype, methods of labeling a nucleic acid-interacting macromolecule, and methods of detecting a nucleic acid-interacting macro-molecule.

Abstract: The present invention provides a method of selecting a compound which inhibits the enzymatic activity of enoyl reductase which comprises: (A) contacting enoyl reductase with the compound linked to an acyl carrier protein; (B) measuring the enzymatic activity of the enoyl reductase of step (A) compared with the enzymatic activity of enoyl reductase in the absence of the compound and selecting the compound which inhibits the enzymatic activity of enoyl reductase.

Abstract: The present invention provides for a method of inhibiting activity of an enoyl reductase enzyme in a cell which comprises contacting the cell with a compound having the structure: 1

Abstract: The present invention provides a method of selecting a compound which inhibits the enzymatic activity of enoyl reductase which comprises: (A) contacting enoyl reductase with the compound linked to an acyl carrier protein; (B) measuring the enzymatic activity of the entoy reductase of step (A) compared with the enzymatic activity of enoyl reductase in the absence of the compound and selecting the compound which inhibits the enzymatic activity of enoyl reductase.

Abstract: The present invention provides a purified and isolated nucleic acid encoding Rhodococcus L-phenylalanine dehydrogenase. The present invention also provides a vector comprising nucleic acid encoding Rhodoccus L-phenylalanine dehydrogenase, a host cell transformed with the vector, and a method for producing recombinant L-phenylalanine dehydrogenase.